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Pharmaceutical composition, anti-tumor pharmaceutical containing same

An anti-tumor drug and composition technology, applied in the field of pharmacy, can solve the problems of high toxicity, difficult to further improve the therapeutic efficiency, easy to induce multidrug resistance, etc., and achieve the effect of wide application prospect.

Inactive Publication Date: 2018-06-01
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the free drug of paclitaxel has the disadvantages of high toxicity and easy induction of multidrug resistance, which makes it difficult to further improve its therapeutic efficiency.

Method used

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  • Pharmaceutical composition, anti-tumor pharmaceutical containing same
  • Pharmaceutical composition, anti-tumor pharmaceutical containing same
  • Pharmaceutical composition, anti-tumor pharmaceutical containing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] This test selects tanshinone Ⅱ A and paclitaxel in the compound concentration range of the pharmaceutical composition described in the present invention to NB 4 Cells (acute promyelocytic leukemia cells) were tested for anti-tumor activity, and the attenuating and synergistic effects of tanshinone IIA and paclitaxel at the compound concentration described in the present invention were verified by comparing with the individual concentration of tanshinone IIA and paclitaxel.

[0042] 1. Test material:

[0043] (1) Cultivation of test cell lines:

[0044] will NB 4 Cells (purchased from Jiangsu KGI Biotechnology Co., Ltd.) were inoculated on RPMI 1640 complete medium in 5% CO 2 , 37°C and saturated humidity conditions, replace the medium every 2 to 3 days, and take the NB in ​​the logarithmic growth phase 4 Cells are tested.

[0045] The RPMI 1640 complete medium contains 10% newborn bovine serum, 100 IU / mL penicillin and 100 μg / L streptomycin.

[0046] (2) Preparatio...

Embodiment 2

[0070] In this test, Tanshinone IIA and paclitaxel were selected to detect the anti-tumor activity of Hepg-2 cells (human liver cancer cells) within the compound concentration range of the pharmaceutical composition described in the present invention. Attenuation and synergism of tanshinone Ⅱ A and paclitaxel at the compound concentration of the present invention.

[0071] 1. Test material:

[0072] (1) Cultivation of test cell lines:

[0073] Hepg-2 cells (from Shanghai University of Traditional Chinese Medicine Teaching and Experimental Center Laboratory) were inoculated on RPMI1640 complete medium in 5% CO 2 , 37°C and saturated humidity conditions, the medium was replaced every 2-3 days, and the Hepg-2 cells in the logarithmic growth phase were used for experiments.

[0074] The RPMI 1640 complete medium contains 10% newborn bovine serum, 100 IU / mL penicillin and 100 μg / L streptomycin.

[0075] (2) Preparation of drug mother solution:

[0076] Tanshinone Ⅱ A powder (pu...

Embodiment 3

[0098] The preparation of embodiment 3 pharmaceutical compositions

[0099] Take the tanshinone IIA raw material drug and prepare a tanshinone IIA solution with a final concentration of 1.5 μM with chloroform; take the paclitaxel raw material drug and prepare a paclitaxel solution with a final concentration of 0.3 μM with chloroform; mix the above-mentioned tanshinone IIA solution with the paclitaxel solution to obtain a drug combination (the ratio of paclitaxel and tanshinone IIA is 1:5)

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Abstract

The invention relates to the pharmaceutical field, and provides a pharmaceutical composition. The pharmaceutical composition comprises a component A and a component B. The component A is tanshinone IIA and / or a derivative of the tanshinone IIA. The component B is paclitaxel and / or a derivative of the paclitaxel. Under the dosage of 1-20:1-20 of the molar ratio, the tanshinone IIA (TanIIA) and / or the derivative of the tanshinone IIA are combined with the paclitaxel (Ptx) and / or the derivative of the paclitaxel, the pharmaceutical composition is creatively discovered to have the synergistic effect. Proved by a test, the combination indexes (CI50) of the tanshinone IIA and the paclitaxel to tumor cells are less than 1, namely the tanshinone IIA and the paclitaxel have the synergistic effect through the compounding in the pharmaceutical composition, the higher anti-tumor activity is acquired in the low concentration, and the pharmaceutical composition has the advantages of low toxicity andhigh efficiency.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to a pharmaceutical composition and an antitumor drug comprising the pharmaceutical composition. Background technique [0002] For a long time, medical workers all over the world have made unremitting efforts to find safe and effective antitumor drugs. As an anticancer drug, traditional Chinese medicine has its unique advantages: compared with chemically synthesized drugs, traditional Chinese medicine has the advantages of convenient material acquisition, low price, and less toxic and side effects, and its prevention and treatment of tumors is achieved through multiple targets, multiple channels, and multiple links. . The anti-tumor mechanism of traditional Chinese medicine ingredients mainly includes regulating the immune function of the body, inhibiting the growth of tumor blood vessels, inducing tumor cell apoptosis, inducing tumor cell differentiation, reversing the multi-drug resistanc...

Claims

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Application Information

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IPC IPC(8): A61K31/58A61K31/337A61P35/00
CPCA61K31/337A61K31/58A61K45/06A61K2300/00
Inventor 冯年平黎哲夏晴
Owner SHANGHAI UNIV OF T C M
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