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A system for determining the individual effectiveness of tripterygium glycosides in the treatment of rheumatoid arthritis by the expression of multiple miRNAs

A rheumatoid and expression technology, which is applied in the field of biomedicine, can solve the problems that the effective dose is equivalent to the toxic dose, affects promotion and rational application, and has large individual differences in clinical efficacy.

Active Publication Date: 2020-04-10
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the large individual differences in clinical efficacy and the equivalent effective dose and toxic dose, it has seriously affected the clinical promotion and rational application.

Method used

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  • A system for determining the individual effectiveness of tripterygium glycosides in the treatment of rheumatoid arthritis by the expression of multiple miRNAs
  • A system for determining the individual effectiveness of tripterygium glycosides in the treatment of rheumatoid arthritis by the expression of multiple miRNAs
  • A system for determining the individual effectiveness of tripterygium glycosides in the treatment of rheumatoid arthritis by the expression of multiple miRNAs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This example is used to illustrate the discovery of biomarkers and the establishment of prediction models of the present disclosure.

[0037] Source of cases and sample size: 40 RA patients who met the inclusion criteria in the rheumatology department and outpatient department of Guang'anmen Hospital, China Academy of Chinese Medical Sciences. At the same time, 10 healthy volunteers from the Health Examination Center were selected, and the age and gender matched the disease group.

[0038] Inclusion criteria: in line with the American College of Rheumatology (American College of Rheumatology, ACR) classification criteria for RA in 1987 and the classification criteria for rheumatoid arthritis in 2010 (ACR / EULAR), never received tripterygium glycosides tablet treatment or in the last 4 days Subjects who have not received the treatment of tripterygium glycosides tablets within a week.

[0039] The classification criteria for rheumatoid arthritis revised by the American Co...

Embodiment 2

[0094] This example serves to illustrate the validation of the disclosed biomarkers and predictive models.

[0095] Reselect 60 cases of RA patients as an independent test set, and the inclusion and exclusion criteria are the same as in Example 1; taking Tripterygium wilfordii polyglycoside tablets for 12 weeks, during which regular follow-ups were performed to detect the indicators of standard treatment, and these RA patients were divided into three groups according to the clinical survey scale: Two groups: standard treatment group and non-standard treatment group; real-time quantitative PCR (qRT-PCR) was used to detect hsa-miR-550b-2-5p, hsa-miR-4797-5p, hsa-miR- The expression levels of 6509-5p and hsa-miR-378g further verify the performance of the curative effect prediction model of Tripterygium wilfordii polyglycoside tablets for individualized treatment of RA obtained in Example 1, and the evaluation indicators include prediction accuracy (accuracy, ACC) receiver operatio...

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Abstract

The invention provides a system for determining the individual effectiveness of treating rheumatoid arthritis by tripterygium glycosides tablets through the expression amount of various miRNAs (microRibonucleic Acid). The various miRNAs comprise hsa-miR-550b-2-5p, hsa-miR-4797-5p, hsa-miR-6509-5p and hsa-miR-378g. The invention further provides a molecular marker for determining the individual effectiveness of treating the rheumatoid arthritis by the tripterygium glycosides tablets, application of the molecular marker to preparation of a kit for determining the individual effectiveness of treating the rheumatoid arthritis by the tripterygium glycosides tablets, and application of a reagent for detecting the expression amount of the molecular marker to the preparation of the kit for determining the individual effectiveness of treating the rheumatoid arthritis by the tripterygium glycosides tablets. The invention provides a high-specificity and high-sensitivity molecular marker and a curative effect predication model for individual diagnosis and treatment of the rheumatoid arthritis.

Description

technical field [0001] The present disclosure relates to the field of biomedicine, in particular, to a system for determining the individual effectiveness of tripterygium glycosides tablets in treating rheumatoid arthritis through the expression levels of multiple miRNAs, and a system for determining the effectiveness of tripterygium glycosides tablets in treating rheumatoid arthritis. Molecular markers for the individual effectiveness of rheumatoid arthritis, the use of the molecular markers in the preparation of kits for determining the individual effectiveness of tripterygium glycosides in the treatment of rheumatoid arthritis, and the method for detecting the expression level of the molecular markers The use of the reagent in the preparation of a kit for determining the individual effectiveness of tripterygium glycosides in treating rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis (Rheumatoid arthritis, RA) is a refractory autoimmune disease in th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/106C12Q2600/158C12Q2600/178
Inventor 张彦琼林娜王海隆
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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