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Application of chrysosplenetin to osteoporosis treatment

A technology of cat's eye grass xanthin and osteoporosis, which is applied in the field of biomedicine to achieve the effect of low liver and kidney metabolic reactions and low dosage

Active Publication Date: 2018-05-29
洪郭驹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no application of cat's eye grass xanthin in the treatment of bone metabolism disorders.

Method used

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  • Application of chrysosplenetin to osteoporosis treatment
  • Application of chrysosplenetin to osteoporosis treatment
  • Application of chrysosplenetin to osteoporosis treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0020] 1. Ophthalmoxanthin inhibits RANKL-induced osteoclastogenesis

[0021] The structure and properties of cat's eye xanthin are as follows: figure 1 As shown in A.

[0022] For osteoclast culture, freshly isolated bone marrow macrophages (BMM) were isolated from the bones of C57BL / 6 mice, and the cells were flushed from the marrow cavity of the femur and tibia in a-MEM containing M-CSF. Convert the original BMM to 10 3 cells / well were inoculated into a 96-well plate with a-MEM and M-CSF (25ng / mL), and kept at 37°C in 5% CO 2 overnight. BMM were stimulated with BSAKL (50 ng / mL) and different concentrations of cat's eye (0.5, 1, 2.5, 5, 10, 20 μmol) every 2 days until osteoclasts formed. Fix with 4% paraformaldehyde in phosphate buffered saline (PBS) for 10 min at room temperature and wash 4 times with PBS. TRAcP activity was detected using the number of TRAcP-positive multinucleated cells. TRAcP-positive multinucleated cells with three or more nuclei were scored as os...

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PUM

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Abstract

The invention discloses an application of chrysosplenetin to osteoporosis treatment. The chrysosplenetin has the functions of simultaneously inhibiting osteoclast formation and promoting two-way regulation of osteoblast in osteoporosis, is low in no observed effect level and good in osteoporosis regulation effect. Besides, the chrysosplenetin has low gastrointestinal reaction and liver-kidney metabolic reaction. The intervention approach of chrysosplenetin-related medicines is clear. The application illuminates that a molecular mechanism in a bone microenvironment and a potential pharmacological intervention approach are of great importance for identifying new treatment compounds.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of cat's eye xanthin in treating osteoporosis. Background technique [0002] Osteoporosis is a dynamic pathological condition caused by an imbalance between osteoblast bone formation and osteoclast bone resorption. Maintaining the link between osteoclasts and osteoblasts is critical for maintaining bone mass. Once osteoclast-induced bone resorption exceeds osteoblast-induced bone formation, it will progress to osteoporosis. Osteoporosis is primarily characterized by decreased bone mineral and structural disturbances in the skeletal system. Osteoclast and osteoblast differentiation and formation are regulated by several signaling pathways. For the osteoclast system, the receptor activator of NF-κB ligand (RANKL) is a member of the tumor necrosis factor family (TNF) mainly expressed by chondrocytes and osteocytes. RANKL is important for osteoclastogenesis and triggers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P19/10A61P19/08
CPCA61K31/352
Inventor 洪郭驹孙平徐家科魏秋实韩晓蕊何伟施旭光
Owner 洪郭驹
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