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Spray drying production process and application of high-activity bifidobacterium powder

A bifidobacterium and bacteria powder technology, applied in the direction of bifidobacteria, application, bacteria, etc., can solve the problems of cumbersome operation steps, limited improvement, and unsuitability for industrial application, so as to improve survival rate, function, and self-resistance reverse effect

Inactive Publication Date: 2018-05-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the optimization of drying protectant formula, the improvement of drying process conditions and the application of microencapsulation technology can improve the drying survival rate of bifidobacteria to a certain extent, the improvement degree of the above methods is limited, and the operation steps are cumbersome, which is not suitable for large-scale production. Industrial application

Method used

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  • Spray drying production process and application of high-activity bifidobacterium powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the spray drying of pseudo small chain bifidobacteria

[0031] (1) Preparation of Bifidobacterium film fermentation medium:

[0032] Preparation of Pseudomonas bifidobacterium film fermentation medium: yeast powder 5g / L, anhydrous sodium acetate 5g / L, beef extract 10g / L, anhydrous glucose 20g / L, fish meal peptone 10g / L, magnesium sulfate heptahydrate 0.5g / L, dipotassium hydrogen phosphate trihydrate 2.6g / L, manganese sulfate monohydrate 0.25g / L, diammonium hydrogen citrate 2g / L, L-cysteine ​​hydrochloride 0.5g / L, Tween -801g / L, the amount of insoluble fiber added is shown in Table 1.

[0033] (2) Fermentation preparation of bifidobacterium bacterium film: Bifidobacterium is activated 3 generations, according to the inoculation ratio of 3-5%, is transferred in the 10L fermenter, continuously feeds nitrogen, adjusts rotating speed, 37 ℃ constant pH ( pH=6) for 24 hours.

[0034] (3) Collection of bifidobacterium pellicles: Centrifuge the bifidobacteria pe...

Embodiment 2

[0041] (1) Preparation of Bifidobacterium film fermentation medium:

[0042] Preparation of Pseudomonas bifidobacterium film fermentation medium: yeast powder 5g / L, anhydrous sodium acetate 5g / L, beef extract 10g / L, anhydrous glucose 20g / L, fish meal peptone 10g / L, magnesium sulfate heptahydrate 0.5g / L, dipotassium hydrogen phosphate trihydrate 2.6g / L, manganese sulfate monohydrate 0.25g / L, diammonium hydrogen citrate 2g / L, L-cysteine ​​hydrochloride 0.5g / L, Tween -801g / L, soybean powder 10g / L.

[0043] (2) Fermentation preparation of bifidobacterium bacterium film: Bifidobacterium is activated 3 generations, according to the inoculation ratio of 3-5%, is transferred in the 10L fermenter, continuously feeds nitrogen, adjusts rotating speed, 37 ℃ constant pH ( pH=6) for 24 hours.

[0044] (3) Collection of bifidobacterium pellicles: Centrifuge the bifidobacteria pellicle fermentation liquid at 4°C to collect the sludge. The centrifugation conditions are: the rotation speed is...

Embodiment 3

[0048] Embodiment 3: the spray drying of Bifidobacterium longum subspecies longum

[0049] (1) Preparation of Bifidobacterium film fermentation medium:

[0050] Yeast powder 6g / L, anhydrous sodium acetate 4g / L, beef extract 12g / L, anhydrous glucose 25g / L, peptone 12g / L, magnesium sulfate heptahydrate 0.5g / L, dipotassium hydrogen phosphate trihydrate 2.6g / L L, manganese sulfate monohydrate 0.25g / L, diammonium hydrogen citrate 2g / L, L-cysteine ​​hydrochloride 0.5g / L, Tween-801g / L, soybean powder 20g / L.

[0051] (2) Fermentation preparation of bifidobacterium bacterium film: Bifidobacterium is activated 3 generations, according to the inoculation ratio of 3-5%, is transferred in the 10L fermenter, continuously feeds nitrogen, adjusts rotating speed, 37 ℃ constant pH ( pH=6) for 24 hours.

[0052] (3) Collection of bifidobacterium pellicles: Centrifuge the bifidobacteria pellicle fermentation liquid at 4°C to collect the sludge. The centrifugation conditions are: the rotation sp...

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Abstract

The invention relates to a spray drying production process and application of high-activity bifidobacterium powder, and belongs to the technical field of foods. According to the invention, a biological mycoderm technology is applied to fermentation production of the bifidobacterium, and an insoluble dietary fiber is taken as a solid interface to form a mycoderm on the solid interface, so that thespray drying survival rate of the bifidobacterium can be obviously improved and can reach 55% or more, and the maximum number of viable bacteria in bacterial powder can reach 5*10<10>cfu / g or more. The invention solves the key technical problem in the development of high-activity bifidobacterium spray-dried bacterial powder. The prepared bifidobacterium powder with high activity and strong stressresistance can be used in solid beverage, leavens and other products.

Description

technical field [0001] The invention relates to a spray-drying production process and application of highly active bifidobacteria powder, belonging to the technical field of food. Background technique [0002] Bifidobacterium is an inherent flora in the intestines of humans and animals, and plays an important role in the stability of intestinal microecology and the regulation of host physiological functions. Commercially available bifidobacterium live bacteria products include: bifidobacterium solid drinks, bifidobacterium fermented milk products, etc., and most of these products are made from bifidobacterium powder. At present, most of the commercially available bifidobacteria powders are produced by freeze-drying. Although freeze-drying can obtain bifidobacteria powders with a high survival rate, there are high requirements for production equipment and energy consumption during the drying process. High, low yield and other shortcomings, these problems have greatly improve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23L33/125A23C9/123A23G1/42A23G3/36A61K35/745C12R1/01
CPCA23C9/1234A23G1/423A23G3/366A23L33/125A23L33/135C12N1/20A23V2002/00A61K2035/115A23V2400/513A23V2400/515A23V2400/517A23V2400/519A23V2400/535A23V2400/533A23V2200/3202A23V2200/3204A23V2250/284A23V2250/5062
Inventor 陈卫陆文伟翟齐啸崔树茂赵建新张灏
Owner JIANGNAN UNIV
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