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Bionic double-layer sustained-release growth factor film as well as preparation method and application thereof

A growth factor, double-layer technology, applied in the field of regenerative medicine, can solve the problems of inactivation, decrease of growth factor activity, maintenance of effective concentration, etc., and achieve good binding and protection effect

Inactive Publication Date: 2018-05-25
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the half-life of exogenous growth factors injected alone is very short, and they are quickly inactivated in the body, making it difficult to maintain effective concentrations in the local area for a long time
Studies have shown that the local slow release of growth factors can be achieved by using biological scaffolds. However, there are still two major defects in the existing slow release growth factor technology: 1) The composition of biological scaffolds is very different from that of normal extracellular matrix in vivo. The binding and protective effects of the growth factor are lower than those in the body, so that the activity of the growth factor decreases and the function is limited; 2) It can only realize the slow release of the growth factor with one kind of charge (positive charge or negative charge), and cannot release the positive and negative charges at the same time. A variety of growth factors can not simulate the synergistic regulation effect of multiple growth factors in vivo

Method used

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  • Bionic double-layer sustained-release growth factor film as well as preparation method and application thereof
  • Bionic double-layer sustained-release growth factor film as well as preparation method and application thereof
  • Bionic double-layer sustained-release growth factor film as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] First prepare the gelatin containing 3% (w / v, g / ml) alkaline gelatin (jelly strength 150Bloom g), 10% (w / v, g / ml) chitosan (molecular weight 100000kDa), 0.1% (w / v , g / ml) hyaluronic acid (molecular weight 3,000,000 kDa), 10% (v / v) glycerol, 0.2% (w / v, g / ml) sodium carboxymethyl cellulose gel-forming aqueous solution of the positive layer. The positive layer colloidal aqueous solution and formaldehyde aqueous solution are mixed evenly, and the final concentration of formaldehyde is 3% (w / v, g / ml). Then spread the mixed solution on the polytetrafluoroethylene template to carry out the cross-linking reaction. The reaction conditions are temperature 22°C, humidity 20%, and reaction time 6 hours to obtain a positive charge layer. Then, prepare 15% (w / v, g / ml) acid gelatin (jelly strength 150Bloom g), 1% (w / v, g / ml) chitosan (molecular weight 300000kDa), 1% (w / v , g / ml) hyaluronic acid (molecular weight 1000000kDa), 6% (v / v) glycerol, 2% (w / v, g / ml) sodium carboxymethyl cell...

Embodiment 2

[0048] First prepare the gelatin containing 15% (w / v, g / ml) alkaline gelatin (jelly strength 220Bloom g), 1% (w / v, g / ml) chitosan (molecular weight 300000kDa), 1% (w / v , g / ml) hyaluronic acid (molecular weight 1000000kDa), 1% (v / v) glycerin, 2% (w / v, g / ml) sodium carboxymethyl cellulose gel-forming aqueous solution of positive charge layer. The positive layer gelling aqueous solution and the glutaraldehyde aqueous solution are mixed evenly, and the final concentration of glutaraldehyde is 0.01% (w / v, g / ml). Then spread the mixed solution on the polytetrafluoroethylene template to carry out cross-linking reaction. The reaction conditions are temperature 20° C., humidity 20%, and reaction time 8 hours to obtain a positive charge layer. Then, prepare 3% (w / v, g / ml) acid gelatin (jelly strength 250Bloom g), 10% (w / v, g / ml) chitosan (molecular weight 40000kDa), 0.1% (w / v , g / ml) hyaluronic acid (molecular weight 2000000kDa), 5% (v / v) glycerol, 0.7% (w / v, g / ml) sodium carboxymethyl...

Embodiment 3

[0050] First prepare the gelatin containing 15% (w / v, g / ml) alkaline gelatin (jelly strength 220Bloom g), 1% (w / v, g / ml) chitosan (molecular weight 300000kDa), 1% (w / v , g / ml) hyaluronic acid (molecular weight 1000000kDa), 1% (v / v) glycerin, 2% (w / v, g / ml) sodium carboxymethyl cellulose gel-forming aqueous solution of positive charge layer. The positive layer gelling aqueous solution and the glutaraldehyde aqueous solution are mixed evenly, and the final concentration of glutaraldehyde is 0.01% (w / v, g / ml). Then spread the mixed solution on the polytetrafluoroethylene template to carry out cross-linking reaction. The reaction conditions are temperature 20° C., humidity 20%, and reaction time 8 hours to obtain a positive charge layer. Then, prepare 15% (w / v, g / ml) acid gelatin (jelly strength 250Bloom g), 1% (w / v, g / ml) chitosan (molecular weight 300000kDa), 1% (w / v , g / ml) hyaluronic acid (molecular weight 1000000kDa), 1% (v / v) glycerin, 2% (w / v, g / ml) sodium carboxymethyl ce...

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Abstract

The invention discloses a bionic double-layer sustained-release growth factor film. The bionic double-layer sustained-release growth factor film is characterized by consisting of extracellular matrixes in similar bodies, comprises a positive charge layer and a negative charge layer, and can realize simultaneous sustained release of growth factors with negative power and positive power. The invention further provides a preparation method for the bionic double-layer sustained-release growth factor film, and the preparation method comprises the following steps: (1) preparing the positive charge layer into a glue solution; (2) preparing the negative charge layer into a glue solution; and (3) neutralizing, cleaning and drying, thereby obtaining the bionic double-layer film. The bionic double-layer sustained-release growth factor film is simple to operate, is low in cost, and the growth factors with negative power and positive power can be adsorbed to the positive charge layer and the negative charge power of the bionic film in an electrostatic mode separately, and can released in a sustained mode along with gradual degradation of the bionic double-layer film. The bionic double-layer sustained-release growth factor film overcomes the defects of the prior art, and plays an important role in regenerative medical fields such as tissue repair.

Description

technical field [0001] The invention relates to the field of regenerative medicine, in particular to a bionic double-layer slow-release growth factor membrane and its preparation and application. Background technique [0002] Growth factors are a class of polypeptide substances that regulate multiple effects such as cell growth and other cell functions in vivo by binding to specific, high-affinity cell membrane receptors. According to their isoelectric points, growth factors can be divided into positively charged growth factors, such as basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF), platelet-derived growth factor ( PDGF), nerve growth factor (NGF), etc., and negatively charged growth factors, such as acidic fibroblast growth factor (aFGF), epidermal growth factor (EGF), bone morphogenetic protein (BMP), stem cell factor (SCF), liver Cell growth factor (HGF), erythropoietin (EPO), interleukin-1α (IL-1α), et...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/20A61L27/54A61L27/58
CPCA61L27/20A61L27/222A61L27/54A61L27/58A61L2300/414A61L2300/602A61L2430/40C08L89/06C08L5/08C08L1/286
Inventor 孙广炜刘洋张英赵姗
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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