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Zooming optical tweezers dual-photon microscopic imaging device and method

A microscopic imaging and two-photon technology, applied in microscopes, measuring devices, fluorescence/phosphorescence, etc., can solve the problems of high cost, difficult separation of focal planes, slow modulation speed, etc., to reduce observation costs, improve zoom and axial The effect of chromatography speed and simple installation and adjustment

Active Publication Date: 2018-05-18
HARBIN INST OF TECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] In conventional objective-type optical tweezers microscopes, the optical tweezers device and the imaging device are usually located on both sides of the sample, which is convenient for separate focusing. However, in some microscopic observation applications that require the introduction of other environmental variables (such as adding radiation The device is used to study the response mechanism of living cells to radiation), and the optical tweezers and the imaging device are required to be located on the same side of the sample. scanning imaging
Adding a zoom lens or DMD to the optical tweezers system can change the focus position of the optical tweezers to solve the above problems, but the modulation speed is slow or expensive

Method used

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  • Zooming optical tweezers dual-photon microscopic imaging device and method
  • Zooming optical tweezers dual-photon microscopic imaging device and method

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Embodiment 1

[0030] Embodiment 1: as attached figure 1 The present embodiment shown provides a zoom optical tweezers two-photon microscopic imaging device, which is used to quickly switch the axial position to realize three-dimensional tomographic scanning.

[0031] A zoom optical tweezers two-photon microscopic imaging device, including a two-photon illumination module, a two-photon scanning module, a two-photon detection module, an optical tweezers focusing module and an optical tweezers axial focusing module:

[0032] The two-photon lighting module is sequentially as follows according to the direction of light propagation: laser device 1, beam expander 2;

[0033] The two-photon scanning module is as follows according to the direction of light propagation: scanning galvanometer 3, scanning lens 4, tube mirror 1 5, dichroic mirror 1 6, dichroic mirror 2 7, objective lens 1 8;

[0034] The two-photon detection module is as follows according to the direction of light propagation: objectiv...

Embodiment 2

[0045] Embodiment 2: as attached figure 1 and figure 2 The present embodiment shown provides a zoom optical tweezers two-photon microscopic imaging method, which is used to quickly switch the axial position to realize three-dimensional tomographic scanning.

[0046] A zoom optical tweezers two-photon microscopic imaging method, the method is realized based on the zoom optical tweezers two-photon microscopic imaging device described in embodiment 1, specific steps:

[0047] Step a, laser device 1 emits pulsed femtosecond laser light, forms wide-beam parallel light after passing through beam expander 2, and wide-beam parallel light passes through scanning galvanometer 3, scanning lens 4, tube mirror 5, dichroic mirror 1 6, and two After the chromatic mirror two 7 and the objective lens one 8, a focused spot is formed on the tested sample 9, and the focused spot excites the tested sample 9 to emit fluorescence;

[0048] Step b, the second laser 21 emits laser light, passes thr...

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Abstract

The invention provides a zooming optical tweezers dual-photon microscopic imaging device and a method and belongs to the technical field of optical microscopic imaging and optical manipulation. According to the technical characteristics, the device comprises a dual-photon illumination module, a dual-photon scanning module, a dual-photon detection module, an optical tweezers focusing module and anoptical tweezers axial focusing module. According to the zooming optical tweezers dual-photon microscopic imaging device, an axial focusing device composed of a polarizing spectroscope, a quarter waveplate, a low aperture objective lens, a tube mirror and a plane reflecting mirror is additionally arranged in a conventional optical tweezers microscopic system, and axial movement of an optical tweezers focal plane in a common optical path optical tweezers dual-photon microscope is realized, so that a suspension sample is grasped to realize axial movement and dual-photon three-dimensional tomographic scanning imaging is realized. The zooming optical tweezers dual-photon microscopic imaging device has the advantages of simplicity in installation and adjustment, rapid zooming and axial tomography speed and low observation cost.

Description

technical field [0001] The invention relates to a microscopic imaging device and method, in particular to a zoom optical tweezers two-photon microscopic imaging device and method, which can realize the separation and adjustment of the common optical path optical tweezers microsystem, and complete three-dimensional two-photon scanning imaging, which belongs to optical Microscopic imaging and optical manipulation technology. Background technique [0002] In conventional objective-type optical tweezers microscopes, the optical tweezers device and the imaging device are usually located on both sides of the sample, which is convenient for separate focusing. However, in some microscopic observation applications that require the introduction of other environmental variables (such as adding radiation The device is used to study the response mechanism of living cells to radiation), and the optical tweezers and the imaging device are required to be located on the same side of the samp...

Claims

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Application Information

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IPC IPC(8): G01N21/64G02B21/32
CPCG01N21/6456G01N2021/6463G02B21/32
Inventor 刘辰光刘俭陈刚谭久彬
Owner HARBIN INST OF TECH
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