Method for high-density fermentation of selenium-enriched lactobacillus reuteri
A selenium-enriched Lactobacillus reuteri, Lactobacillus reuteri technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria and other directions, can solve the problem of high sodium selenite, slow growth of lactic acid bacteria, affecting bacterial cells Product use and other issues, to achieve the effect of high viable bacterial count, increased bacterial concentration, and increased ability to digest and absorb inorganic selenium
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Embodiment 1
[0037] (1) Inoculate the above-mentioned cultured seeds into a high-density fermentation medium at an inoculation amount of 10% (v / v), and culture them anaerobically for 24 hours. In the present embodiment, the formula of per-density fermentation medium includes the following raw materials: 150g sucrose, 150g yeast extract, 7g ammonium citrate, 9g sodium acetate, 6g dipotassium hydrogen phosphate, 0.6g manganese sulfate, 0.8g magnesium sulfate and 3g Tween 80, adjust the pH to 6.2 with phosphoric acid during preparation to obtain a fermentation medium.
[0038] (2) When the fermentation in step (1) was carried out to 4 hours, 3.3 ml of sodium selenite solution with a concentration of 1 g / L was added to every liter of fermentation medium (i.e. fermentation liquid); 3.3ml of sodium selenite solution with a concentration of 1g / L was added to the base (ie fermentation broth). After 20-24 hours of fermentation, the fermentation is completed to obtain a terminal fermentation liquid...
Embodiment 2
[0042] (1) Inoculate the above-mentioned cultivated seeds into a high-density fermentation medium according to the inoculum amount of 3% (v / v), and culture anaerobically for 20 h. In the present embodiment, the formulation of the fermented medium for each raised density includes the following raw materials: 80g sucrose, 80g yeast extract, 3g ammonium citrate, 5g sodium acetate, 3g dipotassium hydrogen phosphate, 0.4g manganese sulfate, 0.6g magnesium sulfate, 2g Tween 80 and phosphoric acid were used to adjust the pH to 5.5 to obtain a fermentation medium.
[0043] (2) Fermentation in step (1) is carried out to 4h, every liter of fermentation medium (ie fermentation liquid)
[0044] 1.1ml of sodium selenite solution with a concentration of 1g / L was added to the fermentation medium; after 16 hours of fermentation, 1.97ml of sodium selenite solution with a concentration of 1g / L was added per liter of fermentation medium (ie, fermentation broth). After the fermentation is carrie...
Embodiment 3
[0048] (1) Inoculate the above-mentioned cultivated seeds into a high-density fermentation medium according to the inoculum amount of 5% (v / v), and culture them anaerobically for 16 hours. In the present embodiment, the formula of per-density fermentation medium includes the following raw materials: 100g sucrose, 100g yeast extract, 5g ammonium citrate, 7g sodium acetate, 4g dipotassium hydrogen phosphate, 0.4g manganese sulfate, 0.7g magnesium sulfate and 2g Tween 80, adjust the pH to 6.2 with phosphoric acid during preparation to obtain a fermentation medium.
[0049] (2) carrying out to 4h in step (1) fermentation, adding concentration is the sodium selenite solution 3.3ml of 1g / L in every liter of fermentation medium (being fermented liquid); Fermentation is carried out to 16h, every liter of fermentation medium ( That is, the fermented liquid) adding concentration is 2.6ml of sodium selenite solution of 1g / L. After the fermentation is carried out to 20-24h, the fermentat...
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