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Lactobacillus reuteri HI120 of high-expression LAI (linoleic acid isomerase) and application of lactobacillus reuteri HI120

A technology of linoleic acid isomerase and Lactobacillus reuteri, applied in the directions of Lactobacillus, isomerase, cis-trans isomerase, etc., can solve the problems of not being allowed, live bacteria cannot be eaten directly, etc.

Active Publication Date: 2018-05-18
佛山市孛特碧欧微生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, bacteria such as Propionibacterium acnes, Bacillus fibrinolyticus, and Megasphaera escherichia are conditional pathogenic bacteria, which have not been included in the list of edible fungi, and live bacteria cannot be eaten directly
However, the existing regulations do not allow recombinant lactic acid bacteria carrying the LAI gene to be marketed as functional foods

Method used

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  • Lactobacillus reuteri HI120 of high-expression LAI (linoleic acid isomerase) and application of lactobacillus reuteri HI120
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  • Lactobacillus reuteri HI120 of high-expression LAI (linoleic acid isomerase) and application of lactobacillus reuteri HI120

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The isolation and identification of embodiment 1 Lactobacillus reuteri

[0033] 1. Isolation of Lactobacillus reuteri strains

[0034] Take 0.5g of fresh feces from healthy children, put it into 9.5mL MRS medium, mix well, and dilute to 100 times. Take 0.1 mL and spread it on the MRS solid medium, and culture it in an anaerobic incubator at 37°C for 48 hours. Select milky white colonies with bulge in the middle, round and bright periphery, and inoculate them in MRS liquid medium respectively, culture them anaerobically at 37°C for 24 hours, then collect the bacteria for CLA conversion experiment, and screen for high activity of linoleic acid isomerase (LAI) strains and identification of the strains ( figure 1 ).

[0035] 2. Screening of highly active linoleic acid isomerase (LAI) strains

[0036] Inoculate the selected colonies and Lactobacillus reuteri standard strain DSM20016 into the MRS medium containing 1 mg / mL Tween-80, and the bacterial density reaches OD 6...

Embodiment 2

[0046] Embodiment 2 live microbial preparations

[0047] 1. Cultivation of live bacteria solution

[0048] Resuscitate the Lactobacillus reuteri HI120 strain with MRS medium. After the bacteria reach a certain concentration, they are inoculated into the MRS medium at a certain ratio (1:100-500), and the bacteria are collected when the bacterial density reaches OD600 of 0.6-1.0.

[0049] 2. Preparation of live bacterial suspension

[0050] After the bacteria are cultured to a certain density, centrifuge at 5000g-10000g for 5-10 minutes to collect the bacteria, add edible sugar-salt mixed solution or beverage to resuspend, and store in a refrigerator at 4-8°C.

[0051] 3. Preparation of freeze-dried live bacterial preparations

[0052] After the bacteria are cultivated to a certain density, collect the bacteria by centrifugation at 5000g-10000g for 5-10 minutes, add antifreeze protection solution (15% skimmed milk powder, 5% glycerin, 0.9% NaCl) to resuspend and mix in a certa...

Embodiment 3

[0057] Example 3 Application of biological live bacteria preparations in the preparation of food additives, food or medicines with the effect of preventing and / or assisting the treatment of obesity and diabetes

[0058] 1. The application of HI120 live bacteria in the prevention and treatment of DB obesity and diabetes

[0059] Twelve DB mice were randomly divided into two groups: oral administration of DSM20016 strain group (DSM group) and oral administration of HI120 strain group (HI120 group). DSM group and HI120 group were fed with 0.1mL (about 6×109cfu) DSM20016 and HI120 bacteria powder respectively. Once a day, the mice were intervened for a total of 4 weeks. The food intake and body weight of the mice were measured every other day, the fasting blood glucose was measured every week, and the oral glucose tolerance test (OGTT) was tested every two weeks. After 4 weeks, the mice were fasted for 12 hours, their eyeballs were removed to collect peripheral blood, and then t...

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Abstract

The invention discloses lactobacillus reuteri HI120 of high-expression LAI (linoleic acid isomerase). The strain is collected in Guangdong Microbiological Culture Collection Center in November 21st of2016, and the collection number is GDMCC No: 60119. The lactobacillus reuteri HI120 is higher than lactobacillus reuteri standard strains and other probiotics in LAI expression level, and linoleic acid can be converted into conjugated linoleic acid in vivo and in vitro. The invention further discloses a microbiological viable bacterium preparation containing the lactobacillus reuteri HI120, and application of the microbiological viable bacterium preparation in preparation of food additives, food or drugs with the effect of preventing and / or adjunctively treating obese diabetics and colorectalcancer.

Description

technical field [0001] The invention belongs to the technical field of micro-ecological living bacteria preparations, and in particular relates to a strain of Lactobacillus reuteri HI120 highly expressing linoleic acid isomerase LAI and an application thereof. Background technique [0002] Obesity, hyperlipidemia, fatty liver and other diseases caused by abnormal lipid metabolism seriously threaten human health. Long-term abnormal lipid metabolism will inevitably lead to "three high" diseases such as hyperlipidemia, hypertension and hyperglycemia, which are the main factors that further cause serious diseases such as cardiovascular and cerebrovascular accidents, liver cirrhosis, diabetes and malignant tumors, and are harmful to human health great. [0003] The vast majority of abnormal lipid metabolism is caused by poor diet and living habits, and is caused by an imbalance in energy intake and consumption. Among them, excessive fatty acid intake and imbalance of fatty acid...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/90A23L33/135A61K35/747A61P3/10A61P35/00C12R1/225
CPCA23L33/135C12N1/20C12N9/90C12Y502/01005A23V2002/00A61K2035/115C12R2001/225C12N1/205A23V2400/173A23V2200/328A23V2200/308
Inventor 曾位森范宏英孟晓静吴军林
Owner 佛山市孛特碧欧微生物科技有限公司
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