Cell culture reactor

Abstract

The invention discloses a cell culture reactor, which includes a tank, an air inlet pipe and a sampling pipe. The tank is provided with a reaction chamber and an air inlet, an air outlet, a sample adding port and a sampling port that communicate with the reaction chamber. The size of the reaction chamber is gradually reduced from the bottom to top, and the undersurface of the reaction chamber is provided with a tapered projection. One end of the air inlet pipe is located outside the tank and communicates with a bioreactor, and the other end of the air inlet pipe is extended to the bottom of the tapered projection via the air inlet. One end of the sampling pipe is located outside the tank and communicates with the bioreactor, and the other end of the sampling pipe is extended to the bottomof the tapered projection via the sampling port. The air outlet and the sample adding port communicate with the bioreactor, respectively. The cell culture reactor is good in oxygen supply performance,uniform in oxygen distribution, reduced in damaged cells, improved in cell culture efficiency, and low in production cost.

Description

technical field [0001] The invention relates to a cell culture reactor. Background technique [0002] Cell culture reactors for animal cell culture are mainly divided into two categories, namely stirred cell culture reactors and non-stirred cell culture reactors. The difference lies in whether stirring paddles are installed inside the cell culture reactors. The biggest disadvantage of stirring reactors for animal cell culture is that the shear force is large and the cells are easily damaged. Although various improvements have been made, this problem still exists. In contrast, the non-stirred cell culture reactor produces less shear force, which has outstanding advantages in animal cell culture. Non-stirred cell culture reactors generally move the cell culture medium in the reactor by shaking, rolling, rotating, swinging, oscillating, etc., so as to maintain the suspended state of cells and achieve efficient gas exchange. The shape of the current mainstream cell culture rea...

AI Technical Summary

Problems solved by technology

However, after oxygen is sprayed to the bottom of the culture tank, it will be continuously consumed during the ascent process, resulting in a decrease in oxygen concentration from bottom to top. In order to ensure the oxygen supply in the upper layer of culture solution, a slightly excessive amount of air or oxygen is often used. Spraying, however, excessive air sparging often produces foam and bursts bubbles, as does excessive oxygen sparging, which is also toxic to cells

On the other hand, due to structural problems, traditional non-stirred cell culture reactors are difficult to generate a high medium surface area during exercise, which also affects the oxygen absorption efficiency

Due to the deficiencies in oxygen supply of such reactors, more complex control towers and related dissolved oxygen probes are often used to monitor the injection of air or pure oxygen, which not only complicates the operation, but also increases the cost of cell culture and optimization

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