Method for synergistically immobilizing L-arabinose isomerase and ionic liquid

Abstract

The invention provides a method for synergistically immobilizing L-arabinose isomerase and ionic liquid. Acrylic fibers are used as a carrier to synergistically immobilize ion liquid and L-arabinose isomerase. The method comprises the following steps: adding acrylic fibers, a compound I and 30mL of deionized water into a three-opening bottle, refluxing, taking out a fiber test sample, suction filtering, washing by using water, air drying, vacuum drying, and obtaining PANF-I, wherein a molecular formula of the compound I is H2N(CH2)nCH2NH2; stirring the dry PANF-I in an HY acid solution, takingout the formed fiber immobilized ion liquid, suction filtering, sprinkling, air drying, and drying; and adding PANF-II and 5mL of freeze enzyme solution into a cross-linking agent, oscillating cross-linking, pouring out the solution, washing the immobilized enzyme particles separately by using 0.9 percent sodium chloride and deionized water, suction filtering, and drying, thus obtaining the immobilized enzyme, and storing at a low temperature. According to the method, the acrylic fibers are adopted as the carrier to synergistically immobilize the ethylamine nitrate ion liquid and L-arabinoseisomerase. The method is mild in condition, high in yield, simple in method, and easy in mass preparation.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for synergistically immobilizing L-arabinose isomerase and ionic liquid. Background technique [0002] L-arabinose isomerase is an intracellular enzyme that can catalyze the synthesis of rare functional sugars such as D-tagatose and L-ribulose, and has important applications in the fields of food and medicine. Among them, L-ribulose can be converted into L-ribose through one-step biocatalysis. L-ribose has huge potential biological activity in food and medicine, and L-ribulose is the most effective biosynthetic pathway for preparing L-ribose. Among them, L-arabinose isomerase is one of the most effective enzymes for biological production of L-ribulose. In recent years, domestic and foreign studies have reported some microorganisms that can be used to ferment and produce L-arabinose isomerase, but the enzyme production levels of these microorganisms are all low. If L-ribulos...

AI Technical Summary

Problems solved by technology

In recent years, research at home and abroad has reported some microorganisms that can be used to ferment and produce L-arabinose isomerase, but the enzyme production levels of these microorganisms are all low. If L-ribulose is produced in the form of free enzyme, there will be a large amount And it is not conducive to the shortcomings such as separation and purification of products, resulting in high production costs, which is not conducive to the realization of industrialized production

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