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Bacillus siamensis SW-2 as well as preparation and application of microbial agent containing bacillus siamensis SW-2

A technology of bacillus and microbial preparations, which is applied in the field of microorganisms, can solve the problems of bacillus siamese that have not yet been discovered, and achieve the effects of reducing water content, increasing organic matter content, reducing C/N and ammonia nitrogen content

Active Publication Date: 2018-05-11
广州融新科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After literature and patent retrieval, no report on the application of Bacillus siamese as an inoculant to high-temperature composting has been found in the prior art

Method used

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  • Bacillus siamensis SW-2 as well as preparation and application of microbial agent containing bacillus siamensis SW-2
  • Bacillus siamensis SW-2 as well as preparation and application of microbial agent containing bacillus siamensis SW-2
  • Bacillus siamensis SW-2 as well as preparation and application of microbial agent containing bacillus siamensis SW-2

Examples

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Embodiment 1

[0015] The screening of embodiment 1 bacterial strain

[0016] Weigh 10g of compost sample, add 90mL of sterile water, shake on a shaker at 150rpm / min for 15min, and after standing for 30min, take 1mL of supernatant and dilute to 10 -5 -10 -6 , spread 0.1 mL of the diluted culture solution evenly on the LB medium, and incubate at a constant temperature of 30° C. for 3 days. Pick a single colony and streak 3-5 times for isolation and purification, and inoculate it on TSA slant medium for preservation after purification.

[0017] The composition of the LB medium is (g / L): tryptone 10.0; yeast powder 5.0; sodium chloride 10.0; pH 7.2.

[0018] The preserved strain is Bacillus siamensis SW-2, which was preserved in the Guangdong Microbial Culture Collection Center on February 2017, with a preservation number of GDMCC No.60287.

[0019] The identifying characteristics of the Bacillus siamensis SW-2 are:

[0020] Gram-positive, facultatively anaerobic, with periosteum flagella, ...

Embodiment 2

[0021] The preparation of embodiment 2 microbial bacterial agents

[0022] (1) Shake flask culture: Pick a ring of bacterial lawn from the slant of B.siamensis SW-2 and put it into a conical flask filled with 100mL LB liquid medium, and culture it on a shaking table at 37°C and 200rpm / min for 18h. The formula of the LB liquid medium is: tryptone 10.0; yeast powder 5.0; sodium chloride 10.0; pH value 7.2.

[0023] (2) Fermentation in seed tank: put the cultured B. siamensis SW-2 fermentation broth into a 10L fermenter, and ferment and cultivate at 37°C and 200rpm / min for 18 hours; the formula of the fermentation medium is the above-mentioned LB liquid medium.

[0024] (3) Fermentation in a fermenter: put the cultured B. siamensis SW-2 fermentation broth into a fermenter, and ferment at 37°C and 200 rpm / min for 24 hours; the formula of the fermentation medium is the above-mentioned LB liquid medium.

[0025] (4) Bacterial agent preparation: add the fermented liquid fermented in...

Embodiment 3

[0026] The application of embodiment 3 microbial bacterial agents

[0027] The following uses municipal sludge composting as an example to illustrate the application of the microbial agent of the present invention in high-temperature composting.

[0028] The high-temperature aerobic composting material is urban sludge, and the fluffing agent is sawdust and straw. After the sludge, sawdust, and straw are mixed at a ratio of 1:0.8:0.2, the moisture is controlled at about 50%, and the inoculation is carried out at a ratio of 5%. Example 2 The microbial bacterial agent is fully mixed and then stacked and fermented. Simultaneously, no addition of the microbial inoculant of the present invention was used as a control. The composting time is 30 days, the temperature of the compost is detected every day, and the compost is turned once every 5 days. When the compost temperature reaches 65°C, the compost is turned over; every 5 days, the multi-point quartering method is used to take sa...

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Abstract

The invention discloses bacillus siamensis SW-2 with the collection number of GDMCC No.60287 as well as a method for preparing a microbial agent from the strain. The method comprises the steps as follows: after bacillus siamensis SW-2 is subjected to activation culture with an LB liquid medium, a fermentation tank is inoculated with the cultured bacillus siamensis SW-2 for enlargement culture withthe LB liquid medium; a fermentation tank is inoculated with a fermentation liquid obtained after enlargement culture for fermentation culture; a fermentation liquid obtained after fermentation culture is added to an auxiliary solid material, and drying, crushing and packaging are performed. The microbial agent can remarkably promote the temperature rise speed in the early period of composting, increase the fermentation temperature and prolong the high-temperature duration. After composting, the content of organic matter is increased, the content of C / N and ammonia nitrogen is reduced, the moisture content is reduced, the composting degree is high, the effects are significant, and mass application can be realized.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Bacillus siamese and its application in high-temperature aerobic composting of organic waste. Background technique [0002] High-temperature aerobic composting refers to the process of converting waste organic matter into fertilizer through the fermentation of microorganisms under certain moisture, C / N ratio and ventilation conditions under artificial control. Process for stabilizing humus. Traditional high-temperature aerobic composting generally utilizes indigenous microorganisms in compost materials. The number of microorganisms is small and the reproduction cycle is long, which leads to problems such as long fermentation cycle and low fermentation efficiency of organic waste. In order to speed up the fermentation speed, the method of inoculating microbial preparations at the initial stage of composting can increase the population of microorganisms at the initial sta...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C05F17/00C12R1/07
CPCC05F11/08C12N1/20C05F17/20C12N1/205C12R2001/07Y02W30/40
Inventor 林超余震叶万良王跃强
Owner 广州融新科技发展有限公司
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