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Tissue culture propagation method of pleionebulbocodioides

A technique for tissue culture and Orchid uniflorum, which is applied in the field of tissue culture and propagation of Orchid uniflorum, can solve the problems of small number of fruit pods, incomplete development of seed embryos, low pollination rate of pollen and the like.

Inactive Publication Date: 2018-05-11
康美药业(昆明)种质资源有限公司
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Problems solved by technology

[0003] At present, most of the solitary orchids circulating in the market are wild, and a few are artificially cultivated; most of the solitary orchids are propagated by divisions. In the natural environment, the pollen pollination rate is low, and the number of pods grown is also small, and the seed embryos are well developed. Incomplete, can only be seeded in culture medium under sterile conditions for tissue culture

Method used

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  • Tissue culture propagation method of pleionebulbocodioides
  • Tissue culture propagation method of pleionebulbocodioides
  • Tissue culture propagation method of pleionebulbocodioides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-3

[0040] Example 1-3 The primary culture medium of Suanlan

[0041] The raw materials were weighed according to the ratio in Table 1, and the preparation was carried out according to the following steps. The difference was that the ratio of the added raw materials was different, and the first-generation culture medium product of Suanlanlan was prepared. See Table 1 for details:

[0042] Table 1: Raw material ratio table of embodiment 1-3

[0043]

Embodiment 4-6

[0044] Example 4-6 Suanlan subculture medium

[0045] Weigh the raw materials according to the ratio in Table 2 respectively, and prepare according to the following steps, the difference is that the ratio of raw materials added is different, and the subculture culture product of Suanlanlan is prepared, see Table 2 for details:

[0046] Table 2: Raw material ratio table of embodiment 4-6

[0047]

[0048]

Embodiment 7-9

[0049] Example 7-9 Rooting medium of garlic orchid

[0050] The raw materials were weighed according to the ratio in Table 3 respectively, and prepared according to the following steps. The difference is that the ratio of raw materials added is different, and the rooting culture medium product of Suanlan orchid is prepared. See Table 3 for details:

[0051] Table 3: Raw material ratio table of embodiment 7-9

[0052]

[0053]

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Abstract

The invention discloses a tissue culture propagation method of pleionebulbocodioides. The tissue culture propagation method comprises the following steps: selecting an explant, disinfecting the explant, sowing seeds, carrying out subculture, carrying out rooting culture, carrying out seedling exercising, domesticating and transplanting. According to the tissue culture propagation method disclosedby the invention, a plant tissue culture technology is utilized, and the problems that traditional vegetative propagation of the pleionebulbocodioides is low, the development of seed embryos is not complete, the seeds are difficultly developed into complete plants under natural conditions and the like are overcome; meanwhile, the requirements on mass propagation are met, the harvesting of wild resources is reduced and the wild resources are protected.

Description

technical field [0001] The invention relates to a tissue culture seedling raising technique, in particular to a method for tissue culture and multiplication of the orchid albicans. Background technique [0002] Pleione bulbocodioides (Franch.) Rolfe, Orchidaceae only Pleione bulbocodioides, ground or epiphytic herb. It grows in forests at an altitude of 1100-3500 meters or on rocky ground or moss-covered rocks at the edge of forests, and is also common on grassy slopes with slightly shaded gravel. Mainly distributed in the southwest of Sichuan, China (Yuexi, Yanyuan, Shimian, Xichang, Muli), the west to the north of Guizhou (Kaili, Yinjiang, Leishan, Guiyang, Fanjingshan, Panxian, Anlong, Wangmo, Nayong), northwestern to southeastern Yunnan (Gongshan, Weixi, Lijiang, Dali, Yangbi, Yongning, Mengzi, Wenshan), southeastern Tibet (Chawarong), and northern Myanmar. The rhizome is used as medicine, which has the effects of clearing heat and detoxification, reducing swelling and...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 许冬瑾严新顾晓波
Owner 康美药业(昆明)种质资源有限公司
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