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Zein ace Inhibiting Peptide and Its Application as Health Food

A zein, inhibitory activity technology, applied in the directions of plant zein, protein-containing food ingredients, applications, etc., can solve the problem of non-absorption, ignoring the influence of selective absorption by small intestinal epithelial cells, ignoring gastrointestinal digestion and absorption And other issues

Active Publication Date: 2021-04-20
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent "Anti-inflammatory peptide isolated from viscera of wrinkled abalone and its application" (201510594885.7), although the preparation of anti-inflammatory peptide by simulating gastrointestinal digestion method, ignores the influence of small intestinal epithelial cells on its selective absorption
In summary, the current methods for preparing functional polypeptides from zein ignore the digestion and absorption of the gastrointestinal tract, and cannot truly simulate the digestion of bioactive peptides in the gastrointestinal tract and the absorption of polypeptides by small intestinal endothelial cells

Method used

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  • Zein ace Inhibiting Peptide and Its Application as Health Food
  • Zein ace Inhibiting Peptide and Its Application as Health Food
  • Zein ace Inhibiting Peptide and Its Application as Health Food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Ultrasonic treatment of zein: quantitatively weigh zein, add distilled water to prepare a 1% (m / v) suspension, and perform single-frequency (40±2kHz) frequency-sweeping ultrasonic treatment. The ultrasonic treatment time is 30min, the intermittent ratio is 10s / 3s, and the temperature is 25°C.

[0052] Preparation of zein enzymatic hydrolysis solution: adjust the pH of the above protein solution to 8, add alkaline protease, the ratio of enzyme to substrate is 1:20 (w / w), mix evenly, the enzymatic hydrolysis temperature is 50°C, and the enzymatic hydrolysis time is 2h. After enzymolysis, adjust the pH of the mixed solution to 7.0, and inactivate the enzyme in a boiling water bath for 10 minutes, centrifuge to obtain the supernatant, desalt, concentrate, and freeze-dry to powder. The degree of hydrolysis of zein, the conversion rate of protein, and the ACE inhibitory activity of its hydrolyzate were measured.

[0053] Simulated gastrointestinal digestion: Prepare artifici...

Embodiment 2

[0056] Ultrasonic treatment of zein: Quantitatively weigh zein, add distilled water to prepare a 2% (m / v) suspension, and perform dual-frequency (22±2 / 40±2kHz) sweeping ultrasonic treatment. The ultrasonic treatment time is 20min, the intermittent ratio is 10s / 3s, and the temperature is 30°C.

[0057]Preparation of zein enzymatic hydrolysis solution: adjust the pH of the above protein solution to 7.5, add neutral protease, the ratio of enzyme to substrate is 1:30 (w / w), mix evenly, the enzymatic hydrolysis temperature is 55°C, and the enzymatic hydrolysis time is 4h. After enzymolysis, adjust the pH of the mixture to 7.0, inactivate the enzyme in a boiling water bath for 10 minutes, centrifuge to obtain the supernatant, desalt, concentrate, and freeze-dry to powder. The degree of hydrolysis of zein, the conversion rate of protein, and the ACE inhibitory activity of its hydrolyzate were measured.

[0058] Simulated gastrointestinal digestion: Prepare artificial gastric juice a...

Embodiment 3

[0061] Ultrasonic treatment of zein: Quantitatively weigh zein, add distilled water to prepare a 5% (m / v) suspension, and perform dual-frequency (40±2 / 68±2kHz) sweeping ultrasonic treatment. The ultrasonic treatment time is 10min, the intermittent ratio is 10s / 3s, and the temperature is 40°C.

[0062] Preparation of zein enzymatic hydrolysis solution: adjust the pH of the above protein solution to 8, add thermophilic protease, the ratio of enzyme to substrate is 1:50 (w / w), mix evenly, enzymolysis temperature is 70°C, and enzymolysis time is 2h. After enzymolysis, the pH of the mixture was adjusted to 7.0, and the enzyme was inactivated in a boiling water bath for 10 minutes. The supernatant was obtained by centrifugation, desalted, concentrated, and lyophilized into powder. The degree of hydrolysis, protein conversion rate, and ACE inhibitory activity were measured.

[0063] Simulated gastrointestinal digestion: Prepare artificial gastric juice according to the United States ...

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Abstract

The invention discloses a zein ACE inhibitory peptide and its application as a health food, and belongs to the technical field of intensive processing of agricultural products and preparation of functional foods. In the present invention, ultrasonic pretreatment of zein is firstly performed, followed by protease enzymatic hydrolysis to prepare zein ACE inhibitory active peptide, and then the ACE inhibitory activity of zein active peptide is tracked by simulating gastrointestinal tract digestion, and then Caco‑2 Following absorption by cells mimicking the intestinal epithelium, pure zein peptides with high ACE inhibitory activity were characterized after gastrointestinal digestion and absorbed by Caco‑2 cells mimicking the lining of the small intestine. The present invention first studies the absorption rate of zein polypeptide digestion products in the Caco-2 simulated small intestinal wall absorption system; for the first time, it identifies two kinds of zein peptides that have been digested by the gastrointestinal tract and absorbed by Caco-2 cells to simulate the small intestinal wall. ACE-inhibiting active zein functional polypeptides.

Description

technical field [0001] The invention provides a zein ACE inhibitory peptide and its application as a health food, which belongs to the technical field of intensive processing of agricultural products and preparation of functional foods, and can be used as a functional additive for functional foods or medicines. Background technique [0002] Hypertension (hypertension) refers to an increase in systemic arterial blood pressure (systolic and / or diastolic) as the main feature (systolic blood pressure ≥ 140 mm Hg, diastolic blood pressure ≥ 90 mm Hg), which may be accompanied by heart, brain, kidney, etc. Clinical syndrome of functional or organic damage to organs. Hypertension is the most common chronic disease and the main risk factor for cardiovascular and cerebrovascular diseases. At present, there are more than 200 million hypertensive patients in China, so the current situation is very serious. Angiotensin converting enzyme (ACE EC 3.4.15.1) is a Zn-containing 2+ The dip...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/425C12P21/06A23L33/18
CPCA23V2002/00A23L33/18C07K14/425C12P21/06A23V2200/326A23V2250/55
Inventor 马海乐梁秋芳任晓锋
Owner JIANGSU UNIV
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