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Method for producing small molecular dextran, product and application thereof

A technology for dextran and dextranase, applied in biochemical equipment and methods, medical preparations containing active ingredients, glycosylase, etc. Improvement and other issues, to achieve the effects of slowing down product inhibition, improving yield and purity, and reducing usage

Inactive Publication Date: 2018-05-04
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing methods of enzymatic hydrolysis to produce dextran all use batch enzymatic hydrolysis, and the enzymatic hydrolysis process is uncontrollable. In addition, due to the wide range of pore size distribution of commercial ultrafiltration membranes, the molecular weight range of dextran products is wide, and a large amount of reduction is produced at the same time. Sugar by-products reduce product yield and purity, and bring burden to subsequent separation and purification; in addition, there is product inhibition in batch enzymatic hydrolysis, it is difficult to increase product concentration, and dextranase cannot be reused, which increases production costs

Method used

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  • Method for producing small molecular dextran, product and application thereof
  • Method for producing small molecular dextran, product and application thereof
  • Method for producing small molecular dextran, product and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] A method for producing small molecule dextran, such as figure 1 As shown, the specific steps are as follows:

[0051] (1) Add a dextranase solution with a pH of 5 and a concentration of 0.01 g / L into a polyacrylonitrile roll-type ultrafiltration membrane system with a molecular weight cut-off of 10,000 Da, and perform filtration and concentration treatment at an operating pressure of 1.0 MPa and a temperature of 30° C. Such as figure 2 As shown, the dextranase 2 is completely adsorbed on the surface of the ultrafiltration membrane 3 or in the membrane pores; an enzyme membrane reactor is obtained;

[0052] (2) Add pH=5.5, concentration is 100g / L, the solution of the dextran raw material 1 that weight-average molecular weight is 20000Da in the enzymatic membrane reactor gained in step (1), at operating pressure is 0.6MPa, temperature is to carry out under 50 ℃ After filtration, the small molecule dextran product 4 in the permeate was collected, and its average molecul...

Embodiment 2

[0055] A method for producing small molecule dextran, the steps are as follows:

[0056] (1) Add a dextranase solution with a pH of 7 and a concentration of 0.1g / L into a polyethersulfone tubular ultrafiltration membrane system with a molecular weight cut-off of 30,000Da, and perform filtration and concentration treatment at an operating pressure of 0.4MPa and a temperature of 20°C. The dextranase is completely adsorbed on the surface of the ultrafiltration membrane or in the pores of the membrane to obtain an enzyme membrane reactor;

[0057] (2) Add pH=6, concentration is 50g / L, the dextran solution that weight-average molecular weight is 40000Da in the enzymatic membrane reactor gained in step (1), at operating pressure be 0.4MPa, temperature is to filter under 37 ℃, collect The average molecular weight of the small molecule dextran product in the permeate solution is 5100 Da, and the molecular weight distribution breadth index is 1.8. Under the same conditions, the averag...

Embodiment 3

[0060] A method for producing small molecule dextran, the steps are as follows:

[0061] (1) First, add a dextranase solution with a pH of 9 and a concentration of 1 g / L into a polysulfone flat-panel ultrafiltration membrane system with a molecular weight cut-off of 50,000 Da, and carry out filtration and concentration treatment at an operating pressure of 0.1 MPa and a temperature of 50°C, so that the dextran The enzyme is completely adsorbed on the surface of the ultrafiltration membrane or in the pores of the membrane to obtain an enzyme membrane reactor;

[0062] (2) Add pH=7.5, concentration is 10g / L, the dextran solution that weight-average molecular weight is 70000Da to the enzymatic membrane reactor gained in step (1), at operating pressure be 0.1MPa, temperature is to filter under 20 ℃, collect The average molecular weight of the small molecule dextran product in the permeate solution was 7000 Da, and the molecular weight distribution breadth index was 1.8. Under the...

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Abstract

The invention relates to a method for producing small molecular dextran, a product and application thereof. The method comprises the following step: performing synchronous enzymolysis and separation on a dextran raw material in an integrated enzyme membrane reactor to obtain a small molecular dextran product, wherein the integrated enzyme membrane reactor comprises an ultrafiltration membrane system which adsorbs dextranase. The enzyme is immobilized on the ultrafiltration membrane system. The enzyme is firstly fixed to the ultrafiltration membrane system in advance, and the enzyme not only serves as a reaction catalyst, but also plays a role in regulating the pore size distribution of a membrane, so that the stability and the repeated practicality of the enzyme are improved and the production cost is reduced; in addition, the pore size distribution of the ultrafiltration membrane is reduced through enzyme immobilization, so that the molecular weight of the obtained small molecular dextran product is more uniform; the enzyme immobilization and the membrane pore size distribution regulation and control can be achieved through simple ultrafiltration enrichment. The method has the advantages of simple and safe operation, no use of a chemical additive and easy continuous and automatic production.

Description

technical field [0001] The invention relates to the technical field of dextran degradation, in particular to a method for producing small-molecule dextran and its product and application. Background technique [0002] Dextran is a high-molecular glucose polymer with an average relative molecular mass of several thousand to hundreds of thousands of Daltons. Small molecule dextran usually refers to dextran with a weight average molecular weight below 20,000 Da, which can improve microcirculation, increase plasma colloid osmotic pressure and osmotic diuresis. [0003] Iron dextran injection is currently recognized as a better iron supplement injection for piglets at home and abroad. One-time intramuscular injection of 1 mL of iron dextran injection with an iron content of 150 g / L within 1-4 days after birth can meet the needs of piglets throughout the lactation period. Iron needs, prevent iron deficiency anemia in piglets, promote piglet growth, and reduce piglet mortality. H...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04C12N11/08A61K31/721A61P7/08A61P7/06
CPCA61K31/721C12N9/2454C12N11/08C12P19/04C12P19/14C12Y302/01011
Inventor 罗建泉苏子然万印华
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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