Measuring method for lactic dehydrogenase activity based on titanium dioxide film field-effect tube

Abstract

The invention discloses a measuring method for lactic dehydrogenase activity based on a titanium dioxide film field-effect tube. The measuring method comprises the following steps: (1) preparing a titanium dioxide film through a sol-gel method: cleaning a silicon plate, preparing a solution, rotatably coating and annealing; (2) connecting a coating electrode to three electrodes; (3) performing enzyme immobilization; (4) detecting enzyme activity: using KEITHLEY 4200 to test electrical properties of the field-effect tube for enzyme activity detection, testing an I-V curve of lactic dehydrogenase of D-S under different grid voltages, and performing instant detection after dropwise adding lactic acids of different concentrations, detecting on multiple reaction time points, and observing I-V curve changes of D-S at different reaction times. The measuring method does not need test paper, is short in test period, is quick in response, and can be used for realizing quick detection for enzymeactivity. Needed enzyme is low in concentration, is small in size, and can be immobilized enzyme. The measuring method is simple, and provides a novel method for measuring enzyme activity in blood serum.

Description

technical field [0001] The invention relates to a technique for measuring lactate dehydrogenase activity based on a titanium dioxide thin film field effect transistor. Background technique [0002] The occurrence of many diseases in the human body is closely related to the abnormality of enzymes. For example, the lack of tyrosinase will cause albinism, the lack of phenylalanine hydroxylase will lead to phenylketonuria, and the high content of lactate dehydrogenase may be due to Pleural and ascites caused by leukemia, pulmonary infarction and tumor metastasis. Diseases of many tissues and organs often manifest as abnormal enzyme activity in the blood, and the determination of enzyme activity is helpful for the diagnosis and treatment of many diseases. Under normal circumstances, the enzymes that play a role in the tissue are contained in very small amounts in the serum. Only when the tissue is damaged, a large amount of specific marker enzymes will be released into the blood...

AI Technical Summary

Problems solved by technology

Both of these methods need to use large-scale special instruments for detection and consume a large amount of enzymes (usually need to collect 3-4 ml of venous blood)

At the same time, clinically, before the determination of enzyme activity, it needs to go through multiple processes such as collection, separation and storage of blood samples. Improper handling of any process will make the measured value inaccurate.

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