Preparation method of Guangxi camellia nitidissima polysaccharide peptide
A technology of camellia japonica and polysaccharide peptide, which is applied in the field of biological extraction, can solve the problems of large consumption of raw materials, low extraction rate, low purity, and loss of activity, and achieve the effects of improving extraction efficiency, improving purification effect, and improving filtration speed
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Embodiment 1
[0022] Embodiment 1, a kind of preparation method of Guangxi golden camellia polysaccharide peptide
[0023] S1. Degreasing: Wash and pulverize the tea leaves, pass through a 10-mesh sieve to obtain tea powder, take 10 g of the tea powder obtained above, add dichloromethane and ethanol, the volume ratio of dichloromethane and ethanol is 2:1, 60 ° C Reflux for 3 hours to obtain defatted golden camellia;
[0024] S2. Extraction: Add 400 mL of distilled water and 30 mg of cellulase to the defatted golden camellia obtained in step S1, adjust the pH value to 5.5 with a citric acid solution with a mass concentration of 5%, perform enzymatic hydrolysis at 40°C for 1 hour, inactivate the enzyme at high temperature, and treat with a pulsed electric field Afterwards, ultrasonically extract at a temperature of 50°C and a frequency of 25kHz for 0.5h, filter through three layers of 200-mesh gauze, collect the filtrate, add distilled water to the filter residue and extract again in the same...
Embodiment 2
[0030] Embodiment 2, a kind of preparation method of Guangxi golden camellia polysaccharide peptide
[0031] S1. Degreasing: Wash and pulverize the tea leaves, pass through a 20-mesh sieve to obtain tea powder, take 10 g of the tea powder obtained above, add dichloromethane and ethanol, the volume ratio of dichloromethane and ethanol is 3:2, 70°C Reflux for 2 hours to obtain defatted golden camellia;
[0032] S2. Extraction: Add 300 mL of distilled water and 40 mg of cellulase to the defatted golden camellia obtained in step S1, adjust the pH value to 5.5 with a citric acid solution with a mass concentration of 7%, perform enzymatic hydrolysis at 50°C for 1 hour, inactivate the enzyme at high temperature, and treat with a pulsed electric field Afterwards, ultrasonically extract at a temperature of 55°C and a frequency of 40kHz for 1.5 hours, filter through three layers of 200-mesh gauze, collect the filtrate, add distilled water to the filter residue and extract again in the s...
Embodiment 3
[0038] Embodiment 3, a kind of preparation method of Guangxi golden camellia polysaccharide peptide
[0039] S1. Degreasing: Wash and grind the tea leaves, pass through a 20-mesh sieve to obtain tea powder, take 10 g of the tea powder obtained above, add dichloromethane and ethanol, the volume ratio of dichloromethane and ethanol is 5:3, 80 ° C Reflux for 2.5 hours to obtain defatted golden camellia;
[0040] S2. Extraction: Add 500 mL of distilled water and 50 mg of cellulase to the defatted golden camellia obtained in step S1, adjust the pH value to 5.5 with a citric acid solution with a mass concentration of 10%, perform enzymatic hydrolysis at 60° C. for 0.5 h, inactivate the enzyme at high temperature, and pulse electric field After treatment, ultrasonically extract for 2 hours at a temperature of 55°C and a frequency of 55kHz, filter through three layers of 200-mesh gauze, collect the filtrate, add distilled water to the filter residue and extract again in the same way, ...
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