Preparation method of Guangxi camellia nitidissima polysaccharide peptide

A technology of camellia japonica and polysaccharide peptide, which is applied in the field of biological extraction, can solve the problems of large consumption of raw materials, low extraction rate, low purity, and loss of activity, and achieve the effects of improving extraction efficiency, improving purification effect, and improving filtration speed

Active Publication Date: 2018-05-01
广西天昌投资有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing method of extracting the effective components of camellia japonica is high-temperature extraction, and some of the activities of the physiologically active substances with health care functions will be lost, and the extraction rate and purity are low, and the consumption of raw materials per unit product is large
In addition, research on polysaccharide peptides has gradually attracted attention. Guangxi Golden Camellia is a unique tea species in my country. There are very few research reports on it at home and abroad, and there are even fewer studies on the preparation of polysaccharide peptides.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, a kind of preparation method of Guangxi golden camellia polysaccharide peptide

[0023] S1. Degreasing: Wash and pulverize the tea leaves, pass through a 10-mesh sieve to obtain tea powder, take 10 g of the tea powder obtained above, add dichloromethane and ethanol, the volume ratio of dichloromethane and ethanol is 2:1, 60 ° C Reflux for 3 hours to obtain defatted golden camellia;

[0024] S2. Extraction: Add 400 mL of distilled water and 30 mg of cellulase to the defatted golden camellia obtained in step S1, adjust the pH value to 5.5 with a citric acid solution with a mass concentration of 5%, perform enzymatic hydrolysis at 40°C for 1 hour, inactivate the enzyme at high temperature, and treat with a pulsed electric field Afterwards, ultrasonically extract at a temperature of 50°C and a frequency of 25kHz for 0.5h, filter through three layers of 200-mesh gauze, collect the filtrate, add distilled water to the filter residue and extract again in the same...

Embodiment 2

[0030] Embodiment 2, a kind of preparation method of Guangxi golden camellia polysaccharide peptide

[0031] S1. Degreasing: Wash and pulverize the tea leaves, pass through a 20-mesh sieve to obtain tea powder, take 10 g of the tea powder obtained above, add dichloromethane and ethanol, the volume ratio of dichloromethane and ethanol is 3:2, 70°C Reflux for 2 hours to obtain defatted golden camellia;

[0032] S2. Extraction: Add 300 mL of distilled water and 40 mg of cellulase to the defatted golden camellia obtained in step S1, adjust the pH value to 5.5 with a citric acid solution with a mass concentration of 7%, perform enzymatic hydrolysis at 50°C for 1 hour, inactivate the enzyme at high temperature, and treat with a pulsed electric field Afterwards, ultrasonically extract at a temperature of 55°C and a frequency of 40kHz for 1.5 hours, filter through three layers of 200-mesh gauze, collect the filtrate, add distilled water to the filter residue and extract again in the s...

Embodiment 3

[0038] Embodiment 3, a kind of preparation method of Guangxi golden camellia polysaccharide peptide

[0039] S1. Degreasing: Wash and grind the tea leaves, pass through a 20-mesh sieve to obtain tea powder, take 10 g of the tea powder obtained above, add dichloromethane and ethanol, the volume ratio of dichloromethane and ethanol is 5:3, 80 ° C Reflux for 2.5 hours to obtain defatted golden camellia;

[0040] S2. Extraction: Add 500 mL of distilled water and 50 mg of cellulase to the defatted golden camellia obtained in step S1, adjust the pH value to 5.5 with a citric acid solution with a mass concentration of 10%, perform enzymatic hydrolysis at 60° C. for 0.5 h, inactivate the enzyme at high temperature, and pulse electric field After treatment, ultrasonically extract for 2 hours at a temperature of 55°C and a frequency of 55kHz, filter through three layers of 200-mesh gauze, collect the filtrate, add distilled water to the filter residue and extract again in the same way, ...

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Abstract

The invention belongs to the technical field of biological extraction, and in particular relates to a preparation method of Guangxi camellia nitidissima polysaccharide peptide. The preparation methodof the Guangxi camellia nitidissima polysaccharide peptide comprises the steps of performing degreasing pretreatment, extracting, performing alcohol precipitation and purifying. The preparation process of the Guangxi camellia nitidissima polysaccharide peptide is reasonable, the extraction efficiency of the of the Guangxi camellia nitidissima polysaccharide peptide is high and the purity is up to86.3 percent; in addition, the prepared polysaccharide peptide has high anti-oxidation activity, the removal rate of hydroxyl free radical reaches to 73 percent, the removal rate of superoxide anion free radical reaches to 81 percent, and theoretical basis is provided for development of the camellia nitidissima serving as anti-oxidation and anti-ageing health-care food.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a preparation method of Guangxi golden camellia polysaccharide peptide. Background technique [0002] Camellia japonica is golden in color, most of them have waxy luster, crystal and lovely, and the flower shapes are cup-shaped, pot-shaped, bowl-shaped and disc-shaped, etc., with various shapes, beautiful and elegant. Among the camellia group, it is known as the "queen of the tea family" . Camellia Camellia is a medicinal and edible plant with high health care value. The flowers and leaves of Camellia Camellia can be used as medicine or edible. Li Shizhen, a great medical scientist in the Ming Dynasty, recorded in "Compendium of Materia Medica" that "the young leaves of camellia are fried and boiled in water. Amoy, edible, can also be steamed and dried for drinking." Research and analysis found that golden camellia contains more than 400 chemical compo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/36C07K1/30C07K1/16C07K1/14
CPCC07K14/415
Inventor 陈俊丞
Owner 广西天昌投资有限公司
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