Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hydrolysate of water extract of syzygium samarangense, a preparation method and uses thereof

A technology of hydrolyzate and monohydric extract, which is applied in skin care preparations, medical preparations containing active ingredients, drug combinations, etc., and can solve problems such as enzyme inactivation and hindering wound healing

Active Publication Date: 2018-05-01
MHBT CO LTD
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive reactive oxygen species and free radicals will not only inhibit angiogenesis, but also inactivate enzymes involved in the signaling pathway, thereby hindering wound healing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hydrolysate of water extract of syzygium samarangense, a preparation method and uses thereof
  • Hydrolysate of water extract of syzygium samarangense, a preparation method and uses thereof
  • Hydrolysate of water extract of syzygium samarangense, a preparation method and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Example 1. Preparation of bromelain hydrolysate of water extract of Syzygium samarangense

[0100] First, crush the Syzygium samarangense fruit (including peel, pulp and seeds) (3-6g) collected from Fangliao Township, Pingtung County, and then mix the crushed fruit with water at a ratio of 1:1.25 The mixture was mixed and heated at 50° C. for 1 hour, thereby obtaining a lotus mist water extract. Next, the lotus mist water extract was mixed with 1600U / mL bromelain (purchased from Qingwang Biotechnology, product number 1600GD) at a weight ratio of 1:180, and then placed at 45°C The enzymatic hydrolysis reaction was carried out for 4 hours. Afterwards, the obtained reaction mixture was centrifuged and the supernatant liquid was collected, followed by filters with a pore size of 5, 1 and 0.5 μm (purchased from Dexi Industry, the models were PureMax, SCB2000-001 and SP2000- 00A) to filter. Afterwards, the obtained filtrate was freeze-dried to obtain a pineapple enzymatic ...

Embodiment 2

[0102] Example 2. High performance liquid chromatography (HPLC) analysis of pineapple enzyme hydrolyzate of lotus mist water extract

[0103]In order to understand the main component distribution of the pineapple enzymatic hydrolyzate of the lotus mist water extract of the present invention, the pineapple enzymatic hydrolyzate of the lotus mist water extract obtained according to the above embodiment 1 is dissolved in secondary water to be formulated into a product with a concentration of The sample to be tested was 1 mg / mL, and then it was used for high-performance liquid chromatography (HPLC) analysis.

[0104] The HPLC analysis instrument used in this experiment is HP Agilent 1100 series liquid chromatography system (HPAgilent 1100 series liquid chromatography system), and various operating parameters and conditions related to HPLC analysis are shown in Table 1 below.

[0105] Table 1. Operating parameters and conditions for HPLC analysis

[0106]

[0107] figure 1 The...

Embodiment 3

[0108] Example 3. Evaluation of the effect of pineapple enzyme hydrolyzate of lotus mist water extract on antioxidant efficacy (antioxidationefficacy)

[0109] In this example, in order to know whether the pineapple enzymatic hydrolyzate of the lotus mist water extract of the present invention has antioxidant effect, the applicant used it to analyze the ferrous ion chelating ability.

[0110] experimental method:

[0111] First, an appropriate amount of the pineapple enzyme hydrolyzate of the lotus mist water extract obtained in the above Example 1 was added to the secondary water to obtain a solution having a concentration of the pineapple enzyme hydrolyzate of the lotus mist water extract at a concentration of 900 μg / mL. The sample to be tested (that is, the experimental group). Next, take 100 μL of the sample to be tested in the experimental group, and then add 3.7 mL of ethanol and 0.1 mL of 2 mM FeCl 2 4H 2 O solution, followed by mixing for 30 seconds. Afterwards, 0....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a hydrolysate of water extract of syzygium samarangense, and a preparation method thereof. The preparation method comprises: extracting syzygium samarangense fruit by using water to obtain a water extract; and carrying out hydrolysis treatment on the water extract by using bromelain to obtain the hydrolysate of the water extract of syzygium samarangense. According to the present invention, the hydrolysate of the water extract of syzygium samarangense can provide effects of skin whitening, skin moisturizing ability improving, wound healing promoting and anti-oxidation property.

Description

technical field [0001] The invention relates to a hydrolyzate of water extract of Syzygium samarangense and a preparation method thereof. The present invention also relates to using the hydrolyzate of the lotus mist water extract to promote skin whitening, improve skin moisturizing ability, promote wound healing and anti-oxidation. Background technique [0002] The skin is the largest barrier to protect the human individual, it has the function of resisting water loss, pathogenic bacteria and various environmental damages. Exposure to large amounts of ultraviolet rays (ultraviolet, UV), ionizing radiation, drugs or xenobiotics can promote the generation of reactive oxygen species (reactive oxygen species, ROS) and free radicals (free radicals) in the skin. When the amount of accumulated reactive oxygen species and free radicals exceeds the antioxidant capacity of cells or tissues, oxidative stress will be formed. Then, reactive oxygen species and free radicals will react w...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/61A61K8/9789A61Q19/02A61Q19/00A61P17/02A61P17/00
CPCA61K8/97A61K36/61A61K2236/19A61K2236/331A61Q19/00A61Q19/02
Inventor 江春晖
Owner MHBT CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com