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Extraction process of prinsepia utilis royle pigment

An extraction process, a technology of green thorn fruit, applied in the field of extraction technology of green thorn fruit pigment

Inactive Publication Date: 2018-04-24
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the pigment extraction and anti-oxidation of the green thorn fruit, there is no report

Method used

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  • Extraction process of prinsepia utilis royle pigment
  • Extraction process of prinsepia utilis royle pigment
  • Extraction process of prinsepia utilis royle pigment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A kind of extracting process of thorn fruit pigment, comprises the following steps:

[0024] 1. Pretreatment of green thorn fruit raw materials

[0025] The fresh green thorn fruit is collected from Lufeng County, Chuxiong City, Yunnan Province; the green thorn fruit is washed and drained, the core is removed, and it is ground into a paste in a mortar and then put into a beaker for later use.

[0026] 2. Preliminary qualitative determination of pigments to determine the best extraction solvent and maximum absorption wavelength

[0027] Accurately weigh 9 parts of 1g samples, place them in Erlenmeyer flasks, set up 9 groups, add 10mL prepared 1% hydrochloric acid / methanol (V hydrochloric acid: V methanol = 1.87:98.13), 1% hydrochloric acid / ethanol (V hydrochloric acid: V ethanol = 1.86:98.14), 1% hydrochloric acid aqueous solution (V hydrochloric acid: V water = 3:97), 60% methanol aqueous solution (V methanol: V water = 60:40), methanol, ethanol, acetone, petroleum eth...

Embodiment 2

[0070] The mensuration of the in vitro antioxidant activity of the thorn fruit pigment extracted by the technique described in embodiment 1 may further comprise the steps:

[0071] 1. Preparation of pigment solution

[0072] According to the optimal extraction conditions, weigh 1g of the green thorn fruit for pigment extraction, and then dilute to a 50mL volumetric flask for later use.

[0073] 2. Determination of DPPH free radical scavenging ability

[0074] Aspirate 0.5mL pigment solution and 2mL LDPPH methanol solution, vortex shake, place the mixture in dark place at room temperature for 10min, then measure colorimetrically at 517nm, and calculate the scavenging ability of DPPH free radicals according to the obtained standard curve. Utilize ferulic acid to draw standard curve, standard equation is y=0.9406x+28.698 (R 2 =0.969)

[0075] Free radical scavenging ability (%) = [(A 0 -A 1 ) / A 0 ]×100

[0076] A 0 is the contrast absorbance,

[0077] A 1 is the absorba...

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Abstract

The invention discloses an extraction process of prinsepia utilis royle pigment, and belongs to the technical field of extraction of natural pigment. The prinsepia utilis royle pigment is subjected topreliminarily qualitative treatment to determine an optimal extraction solvent and a maximum absorption wavelength, a secondary universal rotary combination design is adopted to optimize the extraction process of the prinsepia utilis royle pigment on the basis of a single-factor experiment, and the in vitro anti-oxidation activity of the prinsepia utilis royle pigment is researched; optimal processes for extraction of the prinsepia utilis royle pigment, obtained by research, are as follows: an extraction solution is 1% hydrochloric acid / ethanol in a volume ratio of 1.86:98.14, the extractiontime is 30 min, the extraction temperature is 80 DEG C, and the liquid-to-material ratio is 25:1; and the determination of the in vitro anti-oxidation activity of the prinsepia utilis royle pigment shows that the prinsepia utilis royle pigment has higher anti-oxidation activity, thereby providing a new idea for development and utilization of prinsepia utilis royle.

Description

technical field [0001] The invention relates to the technical field of natural pigment extraction, in particular to a process for extracting the pigment of thorn fruit. Background technique [0002] Prinsepia utilis Royle (Prinsepia utilis Royle), the Chinese name is Prinsepia utilis Royle, which belongs to Rosaceae and Li subfamily Prinsepia. Economic plants are still in the wild. In Yunnan, it is widely distributed in Lijiang, Diqing, Nujiang, Baoshan, Dali, Chuxiong, Kunming, Zhaotong, Yuxi, Honghe, Qujing and other prefectures and cities. Studies have shown that the nuts of the green thorn fruit can regulate blood lipids and cholesterol, and have good curative effects on hepatitis, gastritis, high blood pressure and heart disease. At the same time, the green thorn fruit oil is also widely used as high-grade edible oil, skin care, beauty, health care etc. [0003] The safety of edible natural pigments is very high, mainly extracted from animal and plant tissues, and ha...

Claims

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Application Information

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IPC IPC(8): C09B61/00A61P39/06A23B7/154
CPCC09B61/00A23B7/154A23V2002/00A23V2200/02A23V2250/21
Inventor 李永强杨士花李晴张一鸣黄勇桦张建平李淳初雅洁吴跃中钏新玲
Owner YUNNAN AGRICULTURAL UNIVERSITY
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