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Method for determining pendimethalin content in agricultural products by liquid chromatography

A pendimethalin and liquid chromatography technology, applied in the field of pendimethalin content, can solve the problems of being too simple, affecting the analysis results of pendimethalin residues and the like, and achieve the effect of increasing the rate

Active Publication Date: 2018-04-20
江苏中宜金大分析检测有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no matter which analysis method is used, most of the pretreatment of crops is too simple, which will affect the analysis results of pendimethalin residues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method utilizing liquid chromatography to measure pendimethalin content in agricultural products, mainly comprising the following steps:

[0034] (1) Steam explosion treatment of agricultural products: Accurately weigh 20g of agricultural products, wash them once with pure water to remove surface impurities, and mix the washed agricultural products, pure water, and compound enzymatic hydrolyzate at a mass ratio of 1:1:0.3 Pack into the steam explosion tank, wherein, the composite enzymatic hydrolysis solution comprises: 1.2% alkaline protease, 1.4% proteinase K, 7.5% α-amylase, 4.0% pectinase, 3.5% xylan in terms of mass percentage Enzymes, 0.5% microbial enzymes, 12.3% cellulase, and the balance is phosphate buffer. The enzymes in the compound enzymatic hydrolysis solution are rich in types and are suitable for a variety of crops. After the preliminary treatment of the compound enzymatic hydrolysis solution, the time for steam explosion treatment can be shortened. U...

Embodiment 2

[0049] A method utilizing liquid chromatography to measure pendimethalin content in agricultural products, mainly comprising the following steps:

[0050] (1) Steam explosion treatment of agricultural products: Accurately weigh 25g of agricultural products, wash them twice with pure water to remove surface impurities, and mix the washed agricultural products, pure water, and compound enzymatic hydrolyzate at a mass ratio of 1:1:0.4 Packed into the steam explosion tank, wherein, the composite enzymatic hydrolysis liquid comprises: 1.8% alkaline protease, 2.0% proteinase K, 8.2% α-amylase, 1.3% pectinase, 3.8% xylan in terms of mass percentage Enzymes, 0.85% microbial enzymes, 13.95% cellulase, and the balance is phosphate buffer. The enzymes in the compound enzymatic hydrolysis solution are rich in types and are suitable for a variety of crops. After the preliminary treatment of the compound enzymatic hydrolysis solution, the time for steam explosion treatment can be shortened....

Embodiment 3

[0065] A method utilizing liquid chromatography to measure pendimethalin content in agricultural products, mainly comprising the following steps:

[0066] (1) Steam explosion treatment of agricultural products: Accurately weigh 30g of agricultural products, wash them twice with pure water to remove surface impurities, and mix the washed agricultural products, pure water, and compound enzymatic hydrolyzate at a mass ratio of 1:1:0.5 Pack into the steam explosion tank, wherein, the composite enzymatic hydrolysis liquid comprises: 2.4% alkaline protease, 2.6% proteinase K, 8.9% α-amylase, 4.6% pectinase, 4.1% xylan in terms of mass percentage Enzymes, 1.2% microbial enzymes, 15.6% cellulase, and the balance is phosphate buffer. The enzymes in the compound enzymatic hydrolysis solution are rich in types and are suitable for a variety of crops. After the preliminary treatment of the compound enzymatic hydrolysis solution, the time for steam explosion treatment can be shortened. Ut...

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Abstract

The invention discloses a method for determining pendimethalin content in agricultural products by liquid chromatography, belonging to the technical field of agricultural product detection. The methodmainly comprises the steps of (1) agricultural product steam explosion treatment: firstly using a composite enzymatic hydrolysate to perform primary treatment, then using alkaline water vapor to perform steam explosion treatment, so as to obtain agricultural product slurry and condensed fluid containing pendimethalin; (2) agricultural product slurry treatment: treating the agricultural product slurry subjected to the steam explosion to obtain supernate; (3) constant volume filtration: mixing the supernate and the condensed fluid, then performing adsorption purification treatment by using a purification microballoon; (4) liquid chromatograph detection; (5) result analysis. In a word, the method provided by the invention is high in analysis speed, high in accuracy rate and low in detectionlimit, the added recovery rate is stabilized at 91% or above, the relative standard deviation is low and the work efficiency is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of agricultural product detection, and in particular relates to a method for measuring pendimethalin content in agricultural products by liquid chromatography. Background technique [0002] Pendimethalin, also known as pendimethalin, Chuyatong, Bicaotong, and Shitianbu, is a contact-killing soil sealing treatment agent. The pure product is orange-yellow crystals, with a melting point of 54-58°C and a vapor pressure of 25°C. 40mPa, density 1.19, solubility in water less than 0.5mg / L, stable in the range of 5-130℃, stable to acid and alkali, slow photodecomposition. It is an excellent selective herbicide for upland crops, and can be widely used in corn, soybean, peanut, cotton, direct-directed upland rice, potato, tobacco, vegetables and other crop fields for weeding. At present, pendimethalin is the third largest herbicide in the world, second only to glyphosate and paraquat in sales, and it is also the larg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14B01J20/24B01J20/28B01J20/30
CPCB01J20/04B01J20/10B01J20/12B01J20/20B01J20/24B01J20/28021G01N30/02G01N30/06G01N30/14G01N2030/146
Inventor 任洪强许柯曾超刘敏敏
Owner 江苏中宜金大分析检测有限公司
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