Primer probe combination and kit for combined inspection of 15 kinds of respiratory tract infection pathogens

Abstract

The invention provides a pathogen inspection reagent, and concretely discloses a primer probe combination and a kit for combined inspection of 15 kinds of respiratory tract infection pathogens. By aiming at specific target sequences of the gene sequence conserved region of klebsiella pneumoniae, haemophilus influenzae, streptococcus pyogenes, staphylococcus aureus, escherichia coli, chlamydia pneumoniae, mycobacterium tuberculosis, stenotrophomonas maltophilia, baumanii, mycoplasma pneumoniae, enterococcus faecalis, ligionella pneumohpillia, streptococcus pneumoniae, bacillus pyocyaneus and mycobacterium abscessus, primers and probes which do not have mutual crossed reaction are designed, so that the problem that detection probes of different pathogens can easily generate mutual influenceor interference is solved; the combination and matching of different primers and probes are ensured; the goal of simultaneously performing efficient specific inspection at the same temperature can also be achieved.

Description

Technical field [0001] The invention relates to a reagent for detecting pathogens, in particular to a primer-probe combination and a kit for joint detection of 15 respiratory tract infection pathogens. Background technique [0002] Among adults and children, fever with respiratory syndrome is the most common acute infectious disease, which is mainly caused by local respiratory tract infections or lesions of tissues and organs outside the respiratory tract caused by a variety of pathogens, including bacteria, viruses, mycoplasma, and chlamydia. [0003] Since the clinical symptoms are all manifested as fever (body temperature ≥38℃), accompanied by fatigue, headache, cough, sore throat, body aches and other upper respiratory symptoms, the diagnosis of various pathogens requires a large number of experimental screenings, and the operation steps are cumbersome, It takes a long time and the work efficiency is low. [0004] At present, common pathogens of community respiratory infections ...

AI Technical Summary

Problems solved by technology

[0006] However, there is currently no effective method that can simultaneously detect the above 15 pathogenic bacteria by PCR and ensure the accuracy and specificity of the detection.

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