A genetic molecular marker and application for fast-growing sea cucumber breeding

A molecular marker, sea cucumber technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. Efficient, targeted results

Active Publication Date: 2018-12-18
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these markers have not undergone further expansion verification and production verification, and the feasibility and accuracy of their application need to be further verified

Method used

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  • A genetic molecular marker and application for fast-growing sea cucumber breeding
  • A genetic molecular marker and application for fast-growing sea cucumber breeding
  • A genetic molecular marker and application for fast-growing sea cucumber breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Acquisition of genetic molecular markers for breeding of sea cucumber growth traits and their verification in the offspring of the family

[0023] A sea cucumber family was constructed, and the 2 parents and 142 offspring of the family were used as the mapping population. With the help of a high-throughput sequencing platform, SLAF markers were developed, and a high-density genetic linkage map of sea cucumber was constructed using HighMap software. After high-throughput sequencing, a total of 264,810 SLAF tags were developed, including 112,322 polymorphic SLAF tags. The average sequencing depth of parents with SLAF tags was 23.67×, and the average sequencing depth of offspring was 5.44×. Through bioinformatics analysis, there are 50,905 tags that can be used for genetic map construction. After quality filtering, 4629 polymorphic tags with high sequencing depth, high integrity and no partial separation were obtained. The screened 4629 SLAF tags were used for ...

Embodiment 2

[0027] Example 2: Verification of Apostichopus japonicus growth-related SNP molecular markers in an expanded population

[0028] According to the flanking sequences on both sides of the SNP160 site obtained by high-throughput sequencing, primers for SNP site amplification detection were designed, and the corresponding primer sequences were SNP160F:5'-TCGCAGGAAACCAACCTTCA-3' and SNP160R:5'-AAGGGAGGGTCAAGGAGGAT-3' . The corresponding sequence was used to type the SNP site in the expanded population by using the HRM small fragment method, and the QTL site verification was carried out in combination with the related trait data of the expanded population. The expanded population used was 10-month-old seedlings from the same batch of large-scale breeding and cultured in the same breeding environment. 106 individuals were randomly selected, and the body weight of the individuals was measured. The genotype detection of individuals is carried out by the HRM method. The amplification d...

Embodiment 3

[0032] Example 3: Application of molecular markers for growth traits-assisted selection breeding in Apostichopus japonicus breeding and production

[0033] During the spring seedling period of A. japonicus, 100 parent references were randomly selected from the cultivated parent references, and the genotypes of 100 reference references at the SNP160 locus were genotyped using HRM technology. The primer sequences used were SNP160F: 5'-TCGCAGGAAACCAACCTTCA-3' and SNP160R: 5'-AAGGGAGGGTCAAGGAGGAT-3', and the amplification detection system was: DNA template 1μL (50ng / μL), 4.5μL 2×ES Taq Master Mix, 0.5 μL (10 μmol / L) each of upstream and downstream primers, 3.5 μL ddH 2 O; PCR reaction program: pre-denaturation at 95°C for 5min; denaturation at 94°C for 30s, annealing at 59.5°C for 30s, extension at 72°C for 30s, a total of 35 cycles; final extension at 72°C for 7min, and storage at 4°C; Melting curve analysis technique (HRM) method was carried out. According to the typing results,...

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Abstract

The invention discloses a genetic molecular mark for fast-growing stichopus japonicus breeding and application thereof, and belongs to the field of aquatic product genetic breeding. The molecular markis an SNP160 mark; the amplification molecular mark is an SNP160 primer pair: SNP160F: 5'-TCGCAGGAAACCAACCTTCA-3' and SNP160R:5'-AAGGGAGGGTCAAGGAGGAT-3'. The mark is used for fast growing the property auxiliary breeding advantage genotype of AA. Meanwhile, the invention also discloses the auxiliary breeding steps of the mark. The effective molecular mark is provided for the economic property of the stichopus japonicus growth in the genetic aspect; when the genetic mark is used for mark auxiliary selection and developing the genetic breeding, the growth speed of seedlings can be effectively accelerated; important significance is realized on improving the culture benefits.

Description

technical field [0001] The invention belongs to the field of aquatic genetics and breeding, and in particular relates to a genetic molecular marker for fast-growing sea cucumber breeding and its application. Background technique [0002] Sea cucumber belongs to Echinodermata phylum Echinodermata and sea cucumber class Holothuroidea, and is one of the important sea treasures in northern my country. In recent years, sea cucumber farming in my country has developed rapidly, and its scale has expanded rapidly. The direct economic output value is about 30 billion yuan. It is the species with the largest single output value in my country's mariculture industry. important way. However, with the continuous expansion of the scale of the sea cucumber industry, a series of bottleneck problems that restrict or potentially restrict the development of the industry, such as germplasm degradation, slow growth, long breeding cycle, poor ability to resist environmental changes, and frequent d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李彬荣小军王印庚廖梅杰刘安然陈贵平范瑞用张正
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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