Familial auricular fibrillation gene diagnosis kit

Abstract

The invention discloses a familial auricular fibrillation gene diagnosis kit, and is characterized in that the invention relates to the diagnosis kit for detecting familial auricular fibrillation genes by a large-scale parallel sequencing platform NGS technique. The kit is mainly characterized in that: A, a capture probe is uniquely designed and prepared and aims at all exon fragments of genes ABCC9, GJA5, KCNA5, KCNE2, KCNJ2, KCNQ1, NPPA and SCN5A; B, a connector with a label sequence is uniquely designed; C, a probe sequence is subjected to PCR amplification by universal primers; and D, a uniquely designed target fragment after mixing is subjected to capture operation.

Description

technical field [0001] The invention belongs to the field of molecular biology, relates to medical diagnosis and biotechnology, relates to an in vitro diagnostic kit for gene mutation of familial atrial fibrillation, and is a gene diagnosis of familial atrial fibrillation applied to the NGS technology of a new generation sequencing platform Reagent test kit. Background technique [0002] Atrial fibrillation refers to the rapid and ineffective contraction of the atrium. It is one of the most common and serious cardiac arrhythmias in clinical practice. With the increase of age, the prevalence rate increases year by year. Statistics show that its incidence among adults is nearly 1% under the age of 40, about 2.3% over the age of 40, and as high as 5% over the age of 65. The harm of atrial fibrillation to patients is not only palpitations and other symptoms of arrhythmia, but it is easy to induce serious complications, such as heart failure, tachycardia cardiomyopathy, stroke a...

AI Technical Summary

Problems solved by technology

[0003] As a traditional sequencing technology, Sanger sequencing can only determine one base sequence in one reaction. Although it is accurate, it is inefficient and the cost of single nucleotide sequencing is high.

For the research of complex diseases with genetic heterogeneity, it is usually necessary to sequence the entire coding regions of several or even dozens of genes to find valuable mutation information, and Sanger sequencing cannot meet the requirements of sequencing

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