Familial auricular fibrillation gene diagnosis kit
A technology for atrial fibrillation and genetic diagnosis, applied in the field of molecular biology, can solve the problems of inability to meet the requirements of sequencing, low efficiency, and high cost of single nucleotide sequencing, so as to increase the clinical detection rate and accuracy, reduce costs, The effect of improving detection accuracy and stability
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[0033] The following examples are only described as examples of the practical application of the present invention, and the practical application of the present invention is not limited to the following examples:
[0034] 1. The present invention is described in further detail and completeness below by means of the examples, but the present invention is not limited to the scope of the examples.
[0035] 2. Use TIANamp Blood DNA Midi Kit (DP332) to extract the genome of the sample to be tested. The volume of elution buffer (TE) is not less than 200 μL, and the OD260 / 280 value reaches 1.8-2.0. Take 2 μL of 25ng / μL DNA as a starting point. start template.
[0036] 3. Quantify the sample with Qubit, and use ddH after quantification 2 O Dilute the g DNA sample to 25 ng / μL.
[0037] 4. Building a database
[0038] 5. DNA fragmentation of genomic samples and addition of adapters and tags
[0039] 6. Add 1.5ml of Ethanol to 4.5ml of TargetCap Stop Solution, and mix thoroughly to m...
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