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Diluent used for boar semen and preparation method thereof

A technology of diluent and pig semen, applied in the field of animal husbandry and reproduction, can solve the problems of strengthening or bactericidal effect without the effect of Rehmannia glutinosa and Scrophulariaceae extract, and without certainty of antiviral effect, so as to avoid or reduce sperm. Loss, finished product stability, good stability effect

Inactive Publication Date: 2018-04-17
GUANGDONG ZHONGNONGLIAN BIOLOGICAL PHARMCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The various combinations of its extracts do not have the effect of rehmannia glutinosa and Scrophularia scrophulariae extracts on their strengthening or bactericidal effects. Possess certainty of antiviral effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Weigh 28g of one molecule of crystalline water glucose, one molecule of crystalline water citric acid 4.4g, and two molecules of crystalline water sodium citrate 12.6g, add 800ml water for injection in turn, stir well, add 2g activated carbon for needle, stir well After heating and boiling for 15 minutes, filter to remove charcoal to obtain a clear filtrate. Add 0.24g of povidone iodine to the filtrate, stir to dissolve, and then add water for injection to make up to 1000ml, stir well, use 0.42μm pore size The microporous filter membrane is filtered, filled, sealed, sterilized by hot pressing at 108°C for 30 minutes, then taken out and left to cool.

Embodiment 2

[0021] Weigh 31g of one molecule of crystalline water glucose, one molecule of crystalline water citric acid 4.9g, and two molecules of crystalline water sodium citrate 13.9g, sequentially add 800ml water for injection, stir well, add 2g activated carbon for needles, stir well After heating and boiling for 15 minutes, filter and remove charcoal to obtain a clear filtrate. Add 0.26g of povidone iodine to the filtrate, stir to dissolve, and then add water for injection to make it to 1000ml, stir well, and use a 0.42μm pore size The microporous membrane is filtered, filled, sealed, sterilized at 105°C for 30 minutes by hot pressing, and then taken out and left to cool.

Embodiment 3

[0023] Weigh 30g of one molecule of crystalline water glucose, one molecule of crystalline water citric acid 4.7g, and two molecules of crystalline water sodium citrate 13.3g, sequentially add 800ml water for injection, fully stir, add 2.5g needle activated carbon, stir After heating and boiling for 15 minutes, filter and remove charcoal to obtain a clear filtrate. Add povidone iodine 0.25g to the filtrate, stir to dissolve, and then add water for injection to make it to 1000ml, stir evenly, with a pore size of 0.42μm The microporous filter membrane is filtered, filled, sealed, sterilized by hot pressing at 106°C for 30 minutes, and then taken out and left to cool.

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Abstract

The invention belongs to the technical field of animal husbandry propagation, and particularly discloses a diluent used for boar semen and a preparation method thereof. The diluent used for boar semenis prepared from 2.85-3.15% of one-molecule crystal water glucose, 0.4465-0.4935% of one-molecule crystal water citric acid, 1.2635-1.3965% of two-molecule crystal water sodium citrate, 0.02375-0.02625% of povidone-iodine, and the balance of water for injection to 100%. The preparation method comprises the following steps: taking glucose, citric acid and sodium citrate of formula amount and sequentially dissolving in the water for injection, adding 0.25% of activated carbon for needle of preparation amount, boiling for 15min, filtering to remove carbon, and then adding povidone-iodine of formula amount for dissolving, adding the water for injection to 100%, uniformly stirring, and after pH and content are tested to be qualified, filtering, potting, and sterilizing at 105-108 DEG C for 30min by hot pressing, thus obtaining the diluent used for boar semen. The invention provides a boar semen diluent product which has a killing effect for bacteria, virus, fungus, protozoa and spore for swine artificial insemination, can effective avoid the risk of infection caused by artificial insemination, and is remarkable in practical effect and economical benefit.

Description

Technical field [0001] The invention belongs to the technical field of animal husbandry breeding, and relates to a diluent for pig semen and a preparation method of the diluent. Background technique [0002] With the development and progress of animal husbandry at any time, scientific breeding and breeding technologies are also in the ascendant. In order to optimize and improve the breeds and strains of pigs, it is difficult to rely on traditional male and female natural mating. For this reason, the majority of animal husbandry workers continue to explore, carry out meaningful technical research, and develop the input of boar semen into estrus. The reproductive technology in the reproductive tract of the sow to achieve the conception of the sow is also called artificial insemination. The popularization and application of this technology has promoted the intensive development of the pig breeding industry. For example, in countries such as the United States, Denmark, Britain, Swed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0215A01N1/0221A01N1/0226
Inventor 刘志超刘剑锋叶更青张奎昌
Owner GUANGDONG ZHONGNONGLIAN BIOLOGICAL PHARMCO
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