Detection method and application of cardiomyocytes based on photonic crystal hydrogel fibers
A technology of photonic crystal fibers and photonic crystals, applied in the field of biomedical material research, to achieve the effects of easy large-scale production, simple detection methods, and low cost
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Embodiment 1
[0039] Example 1 Detection and application of cardiomyocytes based on gelatin methacrylate (GelMA) photonic crystal hydrogel fibers
[0040] 1. Preparation of GelMA photonic crystal hydrogel fiber:
[0041] Photonic crystal hydrogel fibers were prepared by chemical etching method using photonic crystal fiber template and biocompatible hydrogel:
[0042] 1) first disperse the purified silicon dioxide particles monodispersed at 300nm in an ethanol solution to obtain a silicon dioxide ethanol solution with a concentration of silicon dioxide particles of 20wt%;
[0043] 2) Then inject the silica ethanol solution into the capillary (ID 300 μm), and naturally deposit to form a photonic crystal fiber template. Finally, the obtained photonic crystal fiber template is calcined at a high temperature (800°C) to obtain a photonic crystal fiber with good mechanical strength template;
[0044] 3) Soak the photonic crystal fiber template with better mechanical strength in the GelMA hydroge...
example 2
[0054] Example 2 A kind of cardiomyocyte detection based on bovine serum albumin (BSA) photonic crystal hydrogel fiber and its application
[0055] 1. Preparation of BSA photonic crystal hydrogel fiber:
[0056] Photonic crystal hydrogel fibers were prepared by physical dissolution method using photonic crystal fiber template and biocompatible hydrogel:
[0057] 1) Firstly, the purified polystyrene particles dispersed in 100 nm are dispersed in an aqueous solution to obtain a polystyrene aqueous solution with a concentration of 10 wt %;
[0058] 2) Then inject the polystyrene aqueous solution into the capillary (ID 200 μm), and naturally deposit to form the photonic crystal fiber template.
[0059] 3) Soak the photonic crystal fiber template with better mechanical strength in BSA hydrogel prepolymer solution (0.2g / ml) for 2h, and cross-link with glutaraldehyde (2wt%) to obtain the photonic crystal hydrogel hybrid system;
[0060] 4) Finally, absolute ethanol was used to diss...
example 3
[0069] Example 3 Detection and application of cardiomyocytes based on gelatin photonic crystal hydrogel fibers
[0070] 1. Preparation of gelatin photonic crystal hydrogel fibers:
[0071] Photonic crystal hydrogel fibers were prepared by chemical etching method using photonic crystal fiber template and biocompatible hydrogel:
[0072] 1) first disperse the purified silicon dioxide particles monodispersed at 200nm in an ethanol solution to obtain a silicon dioxide ethanol solution with a concentration of silicon dioxide particles of 2 wt %;
[0073] 2) Then inject the silica ethanol solution into the capillary (ID 200μm), and naturally deposit to form a photonic crystal fiber template, and finally perform high-temperature calcination (100°C) on the obtained photonic crystal fiber template to obtain a photonic crystal fiber with better mechanical strength template;
[0074] 3) Soak the photonic crystal fiber template with better mechanical strength in the gelatin hydrogel pre...
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