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Novel method for constructing cerebral ischemia animal model with aggregation of magnetic beads

An animal model and construction method technology, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of inconsistent thrombus simulation, large individual variation, and high mortality, so as to improve the success rate of modeling and avoid direct losses. , the effect of low damage

Inactive Publication Date: 2018-04-13
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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AI Technical Summary

Problems solved by technology

[0003] In clinical experiments, the effects of cerebral ischemia on the body are studied by establishing animal models of cerebral ischemia. The simulation of thrombus formation is not consistent with the actual situation. At the same time, it may cause direct damage to blood vessels, the mortality rate is high, the operation is complicated, the individual variation is large, and the operation is not reversible. Due to the possibility of multiple recurrences of transient cerebral ischemia, it cannot Establishment of a Sustainable Animal Model of Cerebral Ischemia

Method used

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Embodiment Construction

[0022] The present invention will be further described in detail below in conjunction with examples.

[0023] 1. Experimental materials:

[0024] 1.1 Animal material

[0025] SD rats were selected, male, weaned rats (day 21 after birth), purchased from the Animal Center of Fudan University School of Medicine. After the animals were repurchased, they were randomly divided into groups and kept in separate cages. Maintain a quiet and comfortable environment during feeding, allowing them to drink water and eat freely. Two days before the start of the experiment, the experimenters touched and grasped the rats once a day in the morning and afternoon, and let the rats get used to the experimental equipment and equipment, in order to eliminate the stress stimulation caused by the experimental environment to the rats as much as possible and ensure the experimental results. accuracy.

[0026] Rats with a body weight of 230±30 g were selected when the rats were raised to an age of 12...

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Abstract

The invention discloses a novel method for constructing a cerebral ischemia animal model with aggregation of magnetic beads. According to the method, a living animal is injected with buffer solutionsprepared from magnetic nanoparticles, soft filiform magnetic lines are wound around an internal carotid artery by surgical means, so that thrombus is formed in the position where the soft filiform magnetic lines are wound of the living animal for two hours, the constructed cerebral ischemia animal model is highly coincided with a symptom of cerebral ischemia, the damage to the animal model is smaller, and the mortality of the constructed animal model is lower.

Description

technical field [0001] The invention relates to the technical field of animal pharmacological experiments, and more specifically relates to a method for constructing a novel magnetic particle aggregation cerebral ischemia animal model. Background technique [0002] Transient ischemic attack (TTA) is a transient insufficiency of blood supply in the carotid artery or vertebral-basilar artery system, causing focal cerebral ischemia and sudden, transient, reversible neurological dysfunction. The attack lasts for several minutes, and usually recovers completely within 30 minutes. After more than 2 hours, mild neurological deficits are often left, or CT and MRI show signs of brain tissue ischemia. TIA is more common in 34-65 years old, 25.3% of them are over 65 years old, more men than women. The onset is sudden, mostly in the case of body position change, hyperactivity, sudden rotation or flexion and extension of the neck. The onset is without aura, and there are transient neur...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61D7/00
CPCA61D1/00A61D7/00
Inventor 耿武军唐红丽
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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