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Culture method of oudemansiella raphanipes

A cultivation method and black-skinned chicken technology are applied in the field of cultivation of Gallus fruticosa to achieve the effect of reducing influence, simplifying the production process and meeting market demand

Inactive Publication Date: 2018-04-13
四川山水美地农业投资有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern medicine has discovered that in addition to having special effects on hemorrhoids, chicken fir can also prevent colon cancer, lower blood pressure, and enhance human immunity. It is difficult to meet the huge market demand simply by picking wild chicken fir.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Preparation of mother species:

[0023] Tissue collection: under aseptic conditions, separate the fruiting bodies of Gallinarum nigricans, and take the tissue with stable performance and active cells between the cap and the fungus as the fruiting body mycelium;

[0024] Cultivation: transfer the mycelium of the fruiting body to a test tube filled with sterilized liquid culture solution and cultivate it at a temperature of 23°C until a large number of small mycelium balls are suspended in the liquid to obtain the mother species;

[0025] Wherein the composition of said liquid culture solution is: 150ml of potato juice, 20g of agar, 20g of glucose, vitamin B 1 5mg, magnesium sulfate 5mg.

[0026] 2. Preparation of original species:

[0027] Insert the cultivated mother species into a 750ml jar with the original seed medium, and cultivate it at a temperature of 25°C for 25 days to mature to obtain the original seed;

[0028] The composition and mass parts of the ori...

Embodiment 2

[0038] 1. Preparation of mother species:

[0039] Tissue collection: under aseptic conditions, separate the fruiting bodies of Gallinarum nigricans, and take the tissue with stable performance and active cells between the cap and the fungus as the fruiting body mycelium;

[0040] Cultivation: transfer the mycelium of the fruiting body to a test tube filled with sterilized liquid culture solution and cultivate it at a temperature of 25°C until a large number of small mycelium balls are suspended in the liquid to obtain the mother species;

[0041] Wherein the composition of said liquid culture solution is: 200ml of potato juice, 20g of agar, 20g of glucose, vitamin B 1 5mg, magnesium sulfate 5mg.

[0042] 2. Preparation of original species:

[0043] Insert the cultured mother species into a 750ml jar containing the original seed medium, and cultivate it at a temperature of 25°C for 23 days to mature to obtain the original seed;

[0044] The composition and mass parts of the...

Embodiment 3

[0054] 1. Preparation of mother species:

[0055] Tissue collection: under aseptic conditions, separate the fruiting bodies of Gallinarum nigricans, and take the tissue with stable performance and active cells between the cap and the fungus as the fruiting body mycelium;

[0056] Cultivation: Transfer the mycelium of the fruiting body to a test tube filled with sterilized liquid culture solution and cultivate it at a temperature of 24°C until a large number of small mycelium balls are suspended in the liquid to obtain the mother species;

[0057] Wherein the composition of said liquid culture solution is: 250ml of potato juice, 20g of agar, 20g of glucose, vitamin B 1 5mg, magnesium sulfate 5mg.

[0058] 2. Preparation of original species:

[0059] Insert the cultivated mother species into a 750ml jar with the original seed medium, and cultivate it at a temperature of 25°C for 24 days to mature to obtain the original seed;

[0060] The composition and mass parts of the origi...

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PUM

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Abstract

The invention relates to a culture method of oudemansiella raphanipes. The method comprises the following steps of preparation of a mother strain, preparation of an original strain, preparation of a cultivation strain and artificial cultivation. The mother strain is prepared in a mother strain culture medium and inoculated into an original strain culture medium to prepare the original strain, theripe original strain is inoculated into a cultivation strain culture medium to obtain the cultivation strain, and finally oudemansiella raphanipes is prepared through artificial cultivation. Large-scale artificial cultivation of oudemansiella raphanipes is achieved, the large market requirement of people for oudemansiella raphanipes can be met, the production process is simple and easy to implement, and the production cost is low.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a method for cultivating Gallus firtrii fungus. Background technique [0002] Gallopia black skin belongs to the order Agaricaceae, and it is a kind of wild edible fungus that grows in summer and autumn in temperate and subtropical regions. Because its growth and development are closely related to the activities of soil termites, and it is highly dependent on the place of growth, and artificial cultivation requires high difficulty. Therefore, the success rate of artificial cultivation of P. nigricans is low and the cost is high. [0003] However, the meat of the chicken fir mushroom is tender, the smell is strong, and the taste is delicious. It is one of the famous wild edible mushrooms, and the taste of the chicken fir mushroom is the best. Traditional Chinese medicine believes that the fungus is flat and sweet, has the effects of invigorating the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20A01G18/30A01G18/68C05G1/00
CPCC05D3/02C05F1/007C05F5/002C05F11/00
Inventor 吴玲周善龙
Owner 四川山水美地农业投资有限公司
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