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Rapid breeding method based on tissue culture for ligularia kaempferi.

A technology of tissue culture and Wufengcao, which is applied in the field of plant breeding, can solve the problems of large differences in breeding environments, achieve the effects of improving the speed of reproduction and the uniformity of seedlings, reducing costs, and obvious leaf characteristics

Inactive Publication Date: 2018-04-03
上海上房园艺有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large differences in the habits and the required breeding environment between New Zealand hemp and S. mosaicus, the above tissue culture method cannot be directly used in the breeding process of S. mosaic

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for rapid breeding of Aurora mosaicus based on tissue culture, comprising the following steps:

[0033] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0034] 1.1. Bud induction medium: MS+BA 3mg / L+NAA 0.3mg / L;

[0035] 1.2. Adventitious bud proliferation medium: MS+6-BA 1mg / L+NAA 0.1mg / L;

[0036] 1.3. Strong seedling medium: MS+6-BA 0.2mg / L+NAA 0.02mg / L;

[0037] 1.4. Rooting medium: MS+NAA 0.1mg / L;

[0038] The pH of the above-mentioned various media is 5.8, add 30g / L sucrose, 6g / L agar, culture temperature is 25°C, and light is 1200Lx;

[0039] (2) Tissue culture of Aurora mosaicus:

[0040] 2.1. Obtaining sterile materials

[0041] Flower leaves Wufengcao dig out the small buds of the aboveground part in March-April in spring, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercu...

Embodiment 2

[0051] A method for rapid breeding of Aurora mosaicus based on tissue culture, comprising the following steps:

[0052] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0053] 1.1. Bud induction medium: MS+BA 1mg / L+NAA 0.1mg / L;

[0054] 1.2. Adventitious bud proliferation medium: MS+6-BA 0.5mg / L+NAA 0.05mg / L;

[0055] 1.3. Strong seedling medium: MS+6-BA 0.1mg / L+NAA 0.01mg / L;

[0056] 1.4. Rooting medium: MS+NAA 0.05mg / L;

[0057] The pH of the above-mentioned various media is 5.8, add 30g / L sucrose, 6g / L agar, culture temperature is 24°C, and light is 1100Lx;

[0058] (2) Tissue culture of Aurora mosaicus:

[0059] 2.1. Obtaining sterile materials

[0060]Flower leaves Wufengcao dig out the small buds of the aboveground part in March-April in spring, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ me...

Embodiment 3

[0070] A method for rapid breeding of Aurora mosaicus based on tissue culture, comprising the following steps:

[0071] (1) The preparation of the culture medium, including the components and contents of the culture medium at each stage of tissue culture:

[0072] 1.1. Bud induction medium: MS+BA 3mg / L+NAA 0.3mg / L;

[0073] 1.2. Adventitious bud proliferation medium: MS+6-BA 1.5mg / L+NAA 0.15mg / L;

[0074] 1.3. Strong seedling medium: MS+6-BA 0.3mg / L+NAA 0.03mg / L;

[0075] 1.4. Rooting medium: MS+NAA 0.15mg / L;

[0076] The pH of the above-mentioned various media is 5.8, add 30g / L sucrose, 6g / L agar, culture temperature is 26°C, light is 1300Lx;

[0077] (2) Tissue culture of Aurora mosaicus:

[0078] 2.1. Obtaining sterile materials

[0079] Flower leaves Wufengcao dig out the small buds of the aboveground part in March-April in spring, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in 75% ethanol for 30 seconds, soak them in 1‰ mercu...

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Abstract

The invention relates to a rapid breeding method based on tissue culture for ligularia kaempferi. The method comprises the following steps: (1) preparing a bud inducing culture medium, an adventitiousbud proliferation culture medium, a seedling strengthening culture medium, and a rooting culture medium; and (2) performing tissue culture on the ligularia kaempferi. According to the method providedby the invention, through a tissue culture technology the annual propagation coefficient is improved to 1:50000 and the cost is reduced by 0.8-1 yuan / plant compared with those of the prior art; and because the floral leaves of the leaves are a yellow-green chimera, the difficulty of the tissue culture is larger, through adjustment of the ratio of auxin to cytokinin, the characteristics of the leaves are obvious, the propagation speed and the seedling uniformity are greatly improved, and the original female parent characters are better maintained.

Description

technical field [0001] The invention relates to a plant breeding method, in particular to a tissue culture-based method for rapid breeding of Acarina fragrans. Background technique [0002] Dawufengcao mosaic is a plant of the genus Dawufengcao in the family Asteraceae, a perennial evergreen herb with rosette-shaped, kidney-shaped basal leaves and green-yellow chimeric leaves. Flowering in October-November, yellow. Dawufengcao is easy to cultivate, and it is evergreen, and the clusters of green leaves are very beautiful. In the garden, it is often planted in clusters or in flower borders, roadsides, and under trees. The flower-leaved Dawufengcao is tolerant to half-shade, and can be used as a ground cover plant in sparse forests. It is a good flower mirror and garden material. As the species introduced from abroad, the number of introductions is small, the ramets are slow, the market demand is large, and the supply of seedlings is limited. [0003] Chinese patent CN10373...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 陈建华黄建荣金梦怡李霖庞玉华
Owner 上海上房园艺有限公司
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