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Preparation method and application of CGA cross-linked sodium alga acid-gelatin blend membrane joint patch

A sodium alginate and blended membrane technology, applied in the field of medicine, can solve the problems of decreased cell activity and survival rate, restricting the application and development of cartilage tissue engineering, etc.

Inactive Publication Date: 2018-03-30
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, studies have found that the cell viability and survival rate are significantly reduced after the graft constructed from stem cells is placed in the hypoxic environment in the joint cavity, which restricts the application and development of cartilage tissue engineering.

Method used

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  • Preparation method and application of CGA cross-linked sodium alga acid-gelatin blend membrane joint patch
  • Preparation method and application of CGA cross-linked sodium alga acid-gelatin blend membrane joint patch
  • Preparation method and application of CGA cross-linked sodium alga acid-gelatin blend membrane joint patch

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Embodiment 1

[0068] 1.1 Research objectives:

[0069] To explore the efficacy of cartilage tissue constructed in vitro by CGA-treated stem cells in repairing cartilage defects, and to explore its possible mechanism, to provide a theoretical basis for the search for effective treatment of joint injuries, and to clarify and fully develop the pharmacological efficacy of CGA and realize the new use of old drugs Provide experimental basis.

[0070] 1.2 Research content:

[0071] On the basis of the previous work, this project uses the cartilage injury model of the lateral femoral condyle surface of chicken embryos and newborn chickens to carry out the following research:

[0072] -Using in vitro culture methods, through morphological staining, electron microscope observation, immunofluorescence, ELISA, PCR and other methods, to observe the influence of CGA on the aggregation, chondrogenic differentiation and functional formation of chicken embryo chondrocyte stem cells, and the effect of CGA o...

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Abstract

The invention discloses a preparation method and application of a CGA cross-linked sodium alga acid-gelatin blend membrane joint patch, and belongs to the field of medical technology. The preparationmethod of the joint patch comprises the following steps: after selection and trypsinization of chick embryo precartilage stem cells, resuspending a sodium alga acid solution and adding CGA in a cell suspension; after uniform blowing and beating, taking and adding a small amount of the cell suspension into a CaCl2 solution; after sodium alga acid is polymerized with the chick embryo precartilage stem cells to form a 3D compound with stable shape, changing a cartilage induction medium; after culture in vitro for 14 days, obtaining the CGA cross-linked sodium alga acid-gelatin blend membrane joint patch. Cartilage injuries on the surfaces of external condyles of thighbones of newly hatched chicken are taken as models, the joint patch is transplanted to the positions of the cartilage injuries,the restorative effect of the cartilage injuries are observed through observational means such as behavior, iconography and morphology, and possible mechanisms are developed by developing a molecularbiological method, so that experimental evidence is provided for comprehensively developing and using CGA and searching effective joint injury therapeutic methods.

Description

technical field [0001] The invention belongs to the technical field of medicine. A joint patch is prepared by using sodium alginate-gelatin blend film cross-linked CGA technology, which is used for repairing joint defects, especially for joint defects related to osteoarthritis (OA, osteoarthritis). It is of great significance to the prognosis of OA. Specifically relates to a preparation method and application of a cross-linked CGA sodium alginate-gelatin blend film joint patch. Background technique [0002] With the improvement of quality of life and the development of sports career, musculoskeletal and joint-related diseases have attracted increasing attention. Articular cartilage injury is a very common clinical disease, protracted and difficult to heal, and it is a difficult problem in the treatment of orthopedics and sports medicine. [0003] Articular cartilage belongs to hyaline cartilage, its cell density, proliferation and migration ability are relatively low, and ...

Claims

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Application Information

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IPC IPC(8): A61L27/54A61L27/38A61L27/22A61L27/20
CPCA61L27/20A61L27/222A61L27/3817A61L27/3852A61L27/54A61L2300/216A61L2300/412A61L2430/06C08L5/04
Inventor 程欣李柯闫语李培芝汪超杰刘梦张静张萍
Owner JINAN UNIVERSITY
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