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Method of separating adipose mesenchymal stem cells from fat

A technology for stem cells and preadipocytes, which is applied in the field of separation of adipose-derived mesenchymal stem cells, can solve the problems of complex separation process, lack of unified operating specifications, and high transaction, and achieves the effects of good stability, excellent formula and good state.

Inactive Publication Date: 2018-03-23
北京焕生汇生物技术研究院有限公司
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  • Application Information

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Problems solved by technology

The separation process is complicated and the turnover is high
And there is currently no uniform operating standard

Method used

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  • Method of separating adipose mesenchymal stem cells from fat

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Embodiment Construction

[0017] A method for isolating adipose-derived mesenchymal stem cells from fat, comprising the following steps:

[0018] (1) Reagent configuration:

[0019] Cleaning solution: Take PBS495mL and add 5mL double antibody to make 500mL cleaning solution;

[0020] Digestive enzyme: take 100ug collagenase I, dissolve it in 100mL PBSs, filter it and set aside, the enzyme needs to be used one hour after preparation, if not used, store it at -20°C as soon as possible;

[0021] Medium: Take 450mL of DMEM-F12 medium and add 50mL of FBS to make 500mL medium;

[0022] (2) Obtaining adipose tissue: extract 100mL of adipose waste under aseptic conditions in the beauty salon, store at 4°C for 3 hours, and transport to the laboratory;

[0023] (3) Fat acquisition: naturally precipitate the obtained adipose tissue to remove the lower non-fat layer; then centrifuge the upper adipose tissue at 2000rpm for 10 minutes to remove the upper fat layer, and use the cleaning solution to clean the lower ...

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Abstract

A method of separating adipose mesenchymal stem cells from fat is characterized by including the steps of: acquiring fat tissue, acquiring fat, acquiring total preadipocyte, and acquiring adipose mesenchymal stem cells. Compared with the prior art, the method of separating adipose mesenchymal stem cells from fat has following advantages: 1, the produced adipose mesenchymal stem cells have excellent status and stability and have multi-directional differentiation capability; 2, the method is easy to carry out and has good repeatability; and 3, formulas of a culture medium and a digestive enzymeare excellent.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for separating fat mesenchymal stem cells from fat. Background technique [0002] Adipose-derived stem cells (ADSCs) and ADSC pluripotent cells are a kind of stem cells with multipotential differentiation potential isolated from adipose tissue in recent years. It mainly restores the repair function of tissue cells, promotes cell regeneration, restores a youthful appearance, and at the same time fully improves body functions, effectively improves sub-health, premature aging and other diseases, and truly effectively resists aging from the inside out. [0003] At present, the in vitro culture of adipose-derived mesenchymal stem cells is mainly through mechanical treatment of tissues, and then the adipose-derived stem cells are collected by means of enzyme digestion and lysis of red blood cells. The separation process is complicated and the turnover is high. And th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N2509/00
Inventor 姚玲玲杨苗孟得龙宋丹刘得娟
Owner 北京焕生汇生物技术研究院有限公司
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