It is suitable for electrokinetic-microbial synergistic remediation of mixed bacterial agent in petroleum hydrocarbon contaminated soil and its preparation and application
A mixed inoculant and synergistic remediation technology, applied in microorganism-based methods, restoration of contaminated soil, biochemical equipment and methods, etc., can solve problems such as death, loss of microorganisms' reproductive and metabolic functions, and loss of petroleum hydrocarbon metabolic activity, etc. Achieve good adaptability and ensure the effect of metabolic activity
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Embodiment 1
[0030] Example 1 is suitable for the preparation of mixed bacterial agents in electrokinetic-microbial synergistic restoration of petroleum hydrocarbon-contaminated soils
[0031]The bacteria source sample used in this embodiment is collected from the high-salt soil polluted by petroleum hydrocarbons for a long time in the oilfield area. The total salt content in the soil reaches 1.6% (w / w), and 5.0 g of petroleum hydrocarbons are suspended by shaking in 30 ml of inorganic salt medium. Polluted saline soil, shake culture at 160rpm / min for 20min, let it stand for 3min, draw 1ml of the upper layer suspension to the inorganic salt liquid containing three inorganic salts of 100mg / L phenanthrene, 300mg / L n-hexadecane and 100mg / L palmitic acid respectively Enrichment and acclimatization culture in the culture medium for 15 days, after separation and purification by streaking on solid inorganic salt medium, Halomonas xianhei using phenanthrene in PAHs as a metabolizable carbon source,...
Embodiment 2
[0038] Example 2 Application of Mixed Bacteria Agent in Electrokinetic-Microbial Synergistic Repair of Petroleum Hydrocarbon Contaminated Soil
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