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Mixed microbial agent suitable for electric-microbiology collaborative remediation of soil contaminated by petroleum hydrocarbon as well as preparation and application of mixed microbial agent

A mixed bacterial agent and synergistic repair technology, applied in the restoration of contaminated soil, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as death, loss of microbial reproduction and metabolism, and loss of metabolic activity of petroleum hydrocarbons. Achieve good adaptability and ensure metabolic activity

Active Publication Date: 2018-03-23
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the electrokinetic-microorganism synergistic restoration technology still has a trend of extreme soil properties. Among them, the high-salinity feature caused by the "ion wall" phenomenon at the electrode end is one of the remarkable characteristics of the soil property change caused by the application of this technology.
High-salt characteristics produce salt stress, causing many microorganisms to dehydrate and lose their reproductive and metabolic functions, and even die in large numbers, losing their petroleum hydrocarbon metabolic activity

Method used

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  • Mixed microbial agent suitable for electric-microbiology collaborative remediation of soil contaminated by petroleum hydrocarbon as well as preparation and application of mixed microbial agent
  • Mixed microbial agent suitable for electric-microbiology collaborative remediation of soil contaminated by petroleum hydrocarbon as well as preparation and application of mixed microbial agent
  • Mixed microbial agent suitable for electric-microbiology collaborative remediation of soil contaminated by petroleum hydrocarbon as well as preparation and application of mixed microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 is suitable for the preparation of mixed bacterial agents in electrokinetic-microbial synergistic restoration of petroleum hydrocarbon-contaminated soils

[0031]The bacteria source sample used in this embodiment is collected from the high-salt soil polluted by petroleum hydrocarbons for a long time in the oilfield area. The total salt content in the soil reaches 1.6% (w / w), and 5.0 g of petroleum hydrocarbons are suspended by shaking in 30 ml of inorganic salt medium. Polluted saline soil, shake culture at 160rpm / min for 20min, let it stand for 3min, draw 1ml of the upper layer suspension to the inorganic salt liquid containing three inorganic salts of 100mg / L phenanthrene, 300mg / L n-hexadecane and 100mg / L palmitic acid respectively Enrichment and acclimatization culture in the culture medium for 15 days, after separation and purification by streaking on solid inorganic salt medium, Halomonas xianhei using phenanthrene in PAHs as a metabolizable carbon source,...

Embodiment 2

[0038] Example 2 Application of Mixed Bacteria Agent in Electrokinetic-Microbial Synergistic Repair of Petroleum Hydrocarbon Contaminated Soil

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Abstract

The invention belongs to the technical field of electric-microbiology collaborative remediation of soil contaminated by petroleum hydrocarbon, and particularly relates to a mixed microbial agent suitable for electric-microbiology collaborative remediation of high-salinity soil contaminated by the petroleum hydrocarbon as well as preparation and application of the mixed microbial agent. The microbial agent is obtained by respectively fermenting and mixing halomonas xianhensis, bacillus licheniformis and bacillus cereus. The combined microbial agent disclosed by the invention is applied to an electric-microbiology collaborative remediation technology; the remediation bottleneck of the high-salinity state of the soil, formed by the remediation process, is broken, functional microbial agent resources are reasonably utilized, degradation of the petroleum hydrocarbon in petroleum oil is effectively realized, and the toxicity of petroleum hydrocarbon organic pollutants in the soil is reduced.

Description

technical field [0001] The invention belongs to the technical field of electrokinetic-microbial synergistic restoration of petroleum hydrocarbon-contaminated soil, and in particular relates to a mixed bacterial agent suitable for electrokinetic-microbial synergistic restoration of high-salinity petroleum hydrocarbon-contaminated soil and its preparation and application. Background technique [0002] The continuous development and growth of the petroleum economy has increased the demand for petroleum hydrocarbons year by year, and the amount of petroleum hydrocarbons entering the soil due to mining landing, transportation leakage and other reasons has also continued to increase, endangering the natural environment and ecological security. In this regard, the treatment and restoration of oil-contaminated soil has been carried out rapidly and has received more and more attention. At present, petroleum hydrocarbon-contaminated soil remediation technologies based on biological an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/10C12R1/085C12R1/01
CPCB09C1/10C12N1/20
Inventor 郭书海王卅王慧王加宁李凤梅吴波
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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