Mixed lactic acid bacteria preparation for wrapping silage in alpine pastoral regions
A technology of lactic acid bacteria and wrapping, applied in bacteria, application, animal husbandry and other directions, can solve the problems of advancing the phenological period of forage grass and increasing the loss of silage, and achieve the effect of improving fermentation quality, improving digestibility and producing more acid.
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[0026] 1 Materials and methods
[0027] 1.1 Isolation of lactic acid bacteria
[0028] A total of 86 samples of natural silage (old awn wheat and oats) were collected in the alpine pastoral areas of Northwest Sichuan, put into sterile ziplock bags with sterile tweezers, sealed, transported back to the laboratory in a car refrigerator, and stored at -80°C for later use.
[0029] Thaw frozen samples at room temperature on an ultra-clean workbench, take 5g of samples into a 250mL sterile Erlenmeyer flask, add 45mL of 0.9% sterile saline, seal with plastic wrap, shake for 2-3 hours, filter with sterile gauze, and take 1m of the filtrate Carry out serial dilution, take 10 respectively -3 、10 -4 、10 -5 1mL of dilution sample solution was spread in sterile medium. 3-5 flat plates (Φ10cm) were coated for each dilution. The medium was MRS medium, CaCO 3 + MRS compound medium, M17 medium and GYP medium (all mediums are provided by Chengdu Litian Century Technology Co., Ltd., the s...
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