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Highly bioactive extracellular matrix material and preparation method and application thereof

A biologically active, extracellular matrix technology, applied in the field of highly biologically active extracellular matrix materials and their preparation, can solve the problems of cumbersome operation in the oscillation process, and achieve the effects of mechanical strength retention, clean biological load removal, and convenient production

Active Publication Date: 2018-03-23
EXCELLENCE MEDICAL TECH SUZHOU CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The operation of the oscillation process is cumbersome

Method used

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  • Highly bioactive extracellular matrix material and preparation method and application thereof
  • Highly bioactive extracellular matrix material and preparation method and application thereof
  • Highly bioactive extracellular matrix material and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Raw material acquisition: The jejunum was taken out half an hour after the death of large pigs with plant-sourced feed closed-feeding, weighing more than 150 kg. After intracavitary flushing, place in the Changzheng-1 organ preservation solution at 2°C, and keep the temperature below 4°C during transportation.

[0049] Pre-sterilization: the porcine jejunum is connected to the perfusion reaction rod, and the cavity is perfused with 0.1% peracetic acid / 4% ethanol mixed disinfectant solution at a rate of 100ml / min / m, while the cavity is soaked in 0.1% peracetic acid / 4% Ethanol mixed with disinfectant, the treatment time is 5 minutes. It has been confirmed that porcine capsular virus can be completely inactivated after treatment for 5 minutes, the bioburden is reduced to no more than 2CFU / g, the amount of endotoxin is reduced to no more than 20EU / g, and the amount of indicated virus is reduced to 100PFU / g. No more than 10% loss in one step.

[0050] Separation of porcine...

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Abstract

The invention relates to a highly bioactive extracellular matrix material and a preparation method and an application thereof. The material is prepared from raw materials with combined use of ultra-high pressure and cavity internal and external perfusion. The preparation method mainly includes the steps: raw material obtaining and preparation, and pretreatment; extracellular matrix mechanical separation; ultra-high pressure crushing and decellularizing; cell content elution and antigen elimination; and ultimately disinfection, sterilization and preservation. Compared with an extracellular matrix obtained by decellularizing through a mechanical oscillation method and a chemical reagent or biological reagent method, the extracellular matrix obtained by the preparation method has the retention amount of adhesion proteins, growth factors, proteoglycans, glycoproteins, glycosaminoglycans and other bioactive components significantly improved; at the same time, the mechanical strength is wellretained, the decellularizing is thorough, and viruses and other biological supporters are removed cleanly and industrialized production is convenient.

Description

technical field [0001] The invention belongs to the field of biological scaffold materials, in particular to a high biological activity extracellular matrix material and its preparation method and application. Background technique [0002] The extracellular matrix (ECM) component biological patch prepared by decellularization technology is an important development direction of soft tissue repair materials. Related products are widely used in various wounds in foreign countries, such as replacing meninges, pleura, blood vessels, periosteum, pericardium, joint capsule, etc., filling hemostasis when liver and spleen and other solid organs are damaged, strengthening anastomosis, bladder suspension, and pelvic floor reconstruction And the treatment of various complex hernias and abdominal wall defects, such as abdominal wall defects with contamination, infection after implantation of synthetic mesh, secondary surgical treatment of intestinal fistula, prevention of parastomal hern...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/58
CPCA61L27/3633A61L27/3687A61L27/3691A61L27/58A61L2430/40
Inventor 张剑
Owner EXCELLENCE MEDICAL TECH SUZHOU CO LTD
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