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Preparation method of tussah fibroin-collagen composite scaffold

A technology of tussah silk fibroin and collagen, which is applied in the field of preparation of tussah silk fibroin-collagen composite scaffolds, can solve problems such as poor shaping, fast collagen degradation, and insufficient mechanical stress strength, and achieve low production costs , Promote rapid healing, good mechanical stress strength

Inactive Publication Date: 2018-03-20
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen has been widely used as a biomedical material. However, collagen has disadvantages such as rapid degradation, poor shaping, and insufficient mechanical stress strength.

Method used

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  • Preparation method of tussah fibroin-collagen composite scaffold
  • Preparation method of tussah fibroin-collagen composite scaffold
  • Preparation method of tussah fibroin-collagen composite scaffold

Examples

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Embodiment 1

[0031] A preparation method of tussah silk fibroin-collagen composite support, it comprises the following steps:

[0032] Step 1. Preparation of pure collagen solution: extract collagen-rich animal tissue by acid enzyme combination method to obtain crude extract, and then the crude extract is purified by centrifugation, salting out and ultrafiltration in sequence, That is, pure collagen solution is obtained;

[0033] In this embodiment, the collagen-rich animal is derived from one or any combination of two or more of cattle, pigs, sheep, chickens, fish, donkeys, deer, jellyfish or sea cucumbers; the tissues are skin, tendon , bone, cartilage or basement membrane;

[0034] In this embodiment, the enzyme used in the acid enzyme binding method is one or any combination of two or more of pepsin, trypsin, papain, bromelain, chymotrypsin or bovine trypsin;

[0035] In this embodiment, the acid used in the acidase combination method is one or a combination of any two or more of hyd...

Embodiment 2

[0043] A preparation method of tussah silk fibroin-collagen composite support, it comprises the following steps:

[0044] Step 1. Preparation of pure collagen solution: extract collagen-rich animal tissue by acid enzyme combination method to obtain crude extract, and then the crude extract is purified by centrifugation, salting out and ultrafiltration in sequence, That is, pure collagen solution is obtained;

[0045]In this embodiment, the animal rich in collagen is derived from bovine; the tissue is skin;

[0046] In this embodiment, the enzyme used in the acid enzyme binding method is pepsin;

[0047] In the present embodiment, the acid used in the acid enzyme binding method is hydrochloric acid;

[0048] Step 2. Preparation of pure tussah silk fibroin protein solution: put tussah silk into alkaline solution and boil, degumming for 35 minutes, wash with water, and repeat degumming and washing 3 times to obtain degummed tussah silk fiber, and then degummed tussah silk Dry ...

Embodiment 3

[0055] A preparation method of tussah silk fibroin-collagen composite support, it comprises the following steps:

[0056] Step 1. Preparation of pure collagen solution: extract collagen-rich animal tissue by acid enzyme combination method to obtain crude extract, and then the crude extract is purified by centrifugation, salting out and ultrafiltration in sequence, That is, pure collagen solution is obtained;

[0057] In this embodiment, the animal rich in collagen is derived from pig; the tissue is tendon;

[0058] In this embodiment, the enzyme used in the acid enzyme binding method is trypsin;

[0059] In the present embodiment, the acid used in the acid enzyme binding method is sulfuric acid;

[0060] Step 2. Preparation of pure tussah silk fibroin protein solution: put tussah silk into an alkaline solution and boil, degumming for 20 minutes, wash with water, and repeat degumming and washing 2 to obtain degummed tussah silk fibers, and then degummed tussah silk fibers Dr...

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Abstract

The invention relates to the technical field of a macromolecule composite scaffold and particularly relates a preparation method of a tussah fibroin-collagen composite scaffold. The preparation methodcomprises the following steps: step one, preparing a pure collagen solution; step two, preparing a tussah silk fibroin solution; and step three, preparing the tussah fibroin-collagen composite scaffold. The tussah fibroin-collagen composite scaffold prepared by the preparation method has the advantages of slow degradation speed, easy shaping and good mechanical stress intensity, has good moistureretention and gas permeability, keeps the wound surface to be with certain humidity, promotes the rapid healing of a wound, and conforms to the flexibility and tensile property of repairing materials. The prepared composite scaffold has the advantages of high liquid absorption and large liquid absorption capacity, has better water absorption performance compared with the mulberry silk-collagen scaffold in the prior art, and also has the effects of absorbing percolate of the wound surface, and isolating the invasion of outside bacteria so as to prevent wound infection.

Description

technical field [0001] The invention relates to the technical field of polymer composite scaffolds, in particular to a method for preparing tussah silk fibroin-collagen composite scaffolds. Background technique [0002] Tussah is a kind of wild silkworm. It is mainly produced in the northeast of China. China's tussah forest is rich in resources, and the annual output of tussah reaches tens of thousands of tons. Tussah silk is composed of two parallel flat monofilaments, and its main components are silk fibroin and sericin, which are composed of 18 kinds of amino acids. Silk fibroin accounts for about 85% of the total amount of tussah silk. It is a natural polymer fiber protein, rich in Asp and Arg, and also has a certain amount of GRD tripeptide sequence. The good biocompatibility and specific affinity to cells of tussah silk fibroin provide a very important feasibility basis for the development of tussah silk related biomedical materials, and provide a class of high-perfor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/58C08L89/00
CPCA61L27/46A61L27/58C08L89/00C08L2205/025
Inventor 彭新生周艳芳崔碧玲刘雯恩范志强张琛琛梁嘉强梁燕珊温玉莹
Owner GUANGDONG MEDICAL UNIV
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