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Method for extracting riboflavin from riboflavin fermentation liquor

A technology of riboflavin and fermented liquid, applied in the direction of organic chemistry, can solve problems such as loss, achieve the effects of reducing impurities, increasing extraction yield and product content, and saving filtration time

Active Publication Date: 2018-03-16
INNER MONGOLIA KINGDOMWAY PHARMA LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alkali dissolution method makes riboflavin prone to irreversible denaturation reaction, resulting in loss

Method used

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  • Method for extracting riboflavin from riboflavin fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] After mixing 3 parts of diatomite and 97 parts of chitosan evenly, put them into pure water and stir to dissolve, the stirring speed is controlled at 15r / min, and stir for 3h. The agent is ready for use.

[0051] Take 10L of riboflavin fermentation broth (titer 23542mg / l) and heat it to 78°C, keep it warm for 30min, after the heating is completed, cool down to 30°C in a water bath. After cooling down, add 300ml of the above-mentioned flocculant into the fermentation broth while stirring, and the stirring speed is controlled at 5r / min. Then, the flocculated liquid was filtered using a Buchner funnel, and the total filtration time was 30 minutes to obtain 1420 g of filter cake with a yield of 99.3%.

[0052] Add the filtered filter cake into a beaker, add 2500ml of ethanol to the filter cake, stir for 30min, and filter after the stirring is completed. The total filtration time is 10min, and 1200g of filter cake is obtained by filtration with a yield of 99.8%.

[0053] I...

Embodiment 2

[0057] After mixing 5 parts of a mixture of diatomite and activated carbon (wherein the weight of diatomite and activated carbon is 50%) and 95 parts of polyacrylamide evenly, put it into pure water and stir to dissolve, and the stirring speed is controlled at 20r / min. After stirring for 2.5 hours, after the stirring is completed, a flocculant with a mass concentration of polyacrylamide of 0.1% is prepared for use.

[0058] Take 10L riboflavin fermentation liquid (titer 23542mg / l) and heat it to 75°C, keep it warm for 60min, after the heating is completed, cool down to 20°C in a water bath. After cooling down, add 500ml of the above-mentioned flocculant into the fermentation broth while stirring, and the stirring speed is controlled at 10r / min. Then, the flocculated flocculant was filtered using a Buchner funnel, and the total filtration time was 25 minutes to obtain 1500 g of filter cake with a yield of 99.5%.

[0059] Add the filtered filter cake into a beaker, add 3000ml o...

Embodiment 3

[0064] The mixture of 4 parts of perlite and activated carbon (wherein perlite accounts for 40% by weight, and activated carbon accounts for 60% by weight) is mixed with 96 parts of polyalumina evenly, and then put into pure water and stirred to dissolve, the stirring speed is controlled at 20r / min, stirred for 2 hours, and after the stirring was completed, a flocculant with a mass concentration of polymerized alumina of 0.3% was prepared for use.

[0065] Take 10L of riboflavin fermentation broth (titer 23542mg / l) and heat it to 95°C, keep it warm for 30min, after the heating is completed, cool down to 35°C in a water bath. After cooling down, add 700ml of the above-mentioned flocculant into the fermentation broth while stirring, and the stirring speed is controlled at 5r / min. Then, the flocculated flocculant was filtered using a Buchner funnel, and the total filtration time was 32 minutes to obtain 1385 g of filter cake with a yield of 99.3%.

[0066] Add the filtered filt...

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Abstract

The invention discloses a method for extracting riboflavin from riboflavin fermentation liquor. The method comprises the following steps that flocculation and filtration of the fermentation liquor areperformed, wherein the inactivated fermentation liquor is cooled, a flocculant solution is added and stirred, filtration is performed after flocculation is completed, and a filter cake is obtained; the filter cake is dehydrated, wherein a dehydration agent is added into the filter cake and is stirred and homogenized, and then filtration is performed to obtain the dehydrated filter cake; acid solution is performed, wherein stirring is performed for dissolution after a hydrochloric acid solution is added into the dehydrated filter cake, and an acid solution is obtained; membrane filtration is performed, wherein the acid solution enters a membrane filtration system, and microfiltration is performed to obtain filtrate; crystallization is performed, wherein air is introduced for stirring afterthe filtrate is heated, water is added for attenuation, stirring crystallization is performed, and filtration and drying are performed to obtain a riboflavin crystallization product. The yield and product content of the riboflavin extracted by adopting the method are high.

Description

technical field [0001] The invention relates to the field of biological extraction, in particular to a method for extracting riboflavin from riboflavin fermentation broth. Background technique [0002] Riboflavin, also known as vitamin B2, vitamin G or lactoflavin, is a water-soluble B vitamin with the molecular formula C 17 h 20 o 6 N 4 , systematically named 7,8-dimethyl-10-(1', D-ribitolyl)-isoalloxazine. [0003] Riboflavin is one of the indispensable vitamins in biological life activities. It is closely related to the metabolism of carbohydrates, fats and amino acids in the human body, and plays an important role in the growth and development of organisms. Mainly used in clinical angular cheilitis, glossitis, stomatitis, anus, conjunctivitis and seborrheic dermatitis and other diseases caused by lack of riboflavin. Another application field of riboflavin is as a feed additive and cosmetics. [0004] Riboflavin has long been commercially produced. At present, ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D475/14
CPCC07D475/14
Inventor 廖炜程张斌庞毅王炳荣王丽
Owner INNER MONGOLIA KINGDOMWAY PHARMA LTD
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