Preparation method of chlorella concentrate phycocyanin functional beverage

A technology of phycocyanin and chlorella, which is applied in the directions of protein food ingredients, protein composition of unicellular algae, functions of food ingredients, etc., to achieve the effect of small loss of biochemical activity, simple operation, and maintenance of biochemical activity

Inactive Publication Date: 2018-03-06
JIANGXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although there are many researches on the health care function of phycocyanin and chlorella essence, there is no functional drink of phycocyanin and chlorella essence on

Method used

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  • Preparation method of chlorella concentrate phycocyanin functional beverage

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Experimental program
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Effect test

Embodiment 1

[0029] (1) Weigh 40 grams of chlorella powder, add it into a 2L Erlenmeyer flask containing 1200 milliliters of deionized water, stir evenly, and leaching in an autoclave at 121° C. for 40 minutes.

[0030] (2) Centrifuge the leachate with a refrigerated centrifuge at 4°C and a centrifugal force of 8000g for 15 minutes, and the supernatant is the chlorella essence solution.

[0031] (3) The chlorella essence solution is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 8000Da to obtain the chlorella essence solution.

[0032] (4) Distill the chlorella essence solution to 1 / 4 volume under reduced pressure, freeze the concentrated solution in a refrigerator at -20°C, and then freeze-dry it in a freeze dryer to obtain the chlorella essence freeze-dried powder.

[0033] (5) Weigh 100 grams of spirulina powder, add it into a 2-liter beaker containing 1500 milliliters of deionized water, stir evenly, and then let stand at room temperature for 24 hours...

Embodiment 2

[0040] (1) Weigh 30 grams of chlorella powder, add it into a 2L Erlenmeyer flask containing 1200 milliliters of deionized water, stir well, and then leaching in an autoclave at 115° C. for 50 minutes.

[0041] (2) The leaching solution was centrifuged for 20 minutes at 4°C and a centrifugal force of 10,000 g in a refrigerated centrifuge, and the supernatant was the chlorella essence solution.

[0042] (3) The chlorella essence solution is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 10 000 Da to obtain the chlorella essence solution.

[0043] (4) The chlorella essence solution is distilled under reduced pressure to about 1 / 5 volume, the concentrated solution is placed in a refrigerator to freeze at -20° C., and then freeze-dried in a freeze dryer to obtain a chlorella essence freeze-dried powder.

[0044] (5) Weigh 80 grams of spirulina powder, add it into a 2-liter beaker containing 1200 milliliters of deionized water, stir evenly, and the...

Embodiment 3

[0051] (1) Weigh 30 grams of chlorella powder, add it into a 2L Erlenmeyer flask containing 1200 ml of deionized water, stir evenly, and then leaching in an autoclave at 121° C. for 60 minutes.

[0052](2) The leachate was centrifuged with a refrigerated centrifuge at 4°C and a centrifugal force of 10,000g for 20 minutes, and the supernatant was the chlorella essence solution.

[0053] (3) The chlorella essence solution is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 10 000 Da to obtain the chlorella essence solution.

[0054] (4) Distill the chlorella essence solution to about 1 / 5 volume, put the concentrated solution in the refrigerator to freeze at -20°C, and then freeze-dry it in a freeze dryer to obtain the chlorella essence freeze-dried powder.

[0055] (5) Weigh 80 grams of spirulina powder, add it into a 2-liter beaker containing 1200 milliliters of deionized water, stir evenly, and then let stand at room temperature for 24 hours to...

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Abstract

The invention provides a preparation method of chlorella concentrate phycocyanin functional beverage and belongs to the field of biotechnology. The preparation method comprises the following steps: uniformly mixing chlorella and deionized water in a ratio of 1:(20-50), heating mixed liquor for 30-60 minutes at the temperature of 110-121 DEG C, centrifuging for 15-30 minutes, then abandoning precipitates, carrying out ultrafiltration on supernatant, and carrying out freeze drying on ultrafiltrate, so that chlorella concentrate is obtained; mixing spirulina powder and water in a ratio of 1:(10-20), swelling for 24 hours, then stirring and leaching for 2-4 hours at the temperature of 25-40 DEG C and the stirring rotating speed of 100-400rpm, centrifuging a leachate for 15-30 minutes, carryingout ultrafiltration on the supernatant for removing small molecular impurities, so that phycocyanin solution with color value of higher than 0.8 is obtained, carrying out ultrafiltration on the phycocyanin solution, and carrying out free drying on ultrafiltrate, so that phycocyanin powder is obtained; mixing the obtained phycocyanin, the chlorella concentrate, white granulated sugar, food additives such as sodium benzoate and tea polyphenol as well as water, preparing into functional beverage with health function, filtering and removing bacteria with a 0.22 Mum filter membrane, and then packaging. The preparation method provided by the invention is simple and reliable in operation, and industrial production can be carried out.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing a chlorella fine phycocyanin functional drink. Background technique [0002] Spirulina contains a variety of vitamins, proteins, fats, carbohydrates, minerals and trace elements in a scientific and reasonable proportion. It has a wide range of food, health and medicinal values. It can promote human metabolism, improve body immunity, resist radiation, and assist cancer It is an ideal pure natural health food and is recommended as "the most ideal food in the 21st century" by the Food and Agriculture Organization of the United Nations and the World Food Association of the United Nations. It is extracted from spirulina. The active substance is also considered harmless. [0003] Spirulina is rich in phycocyanin (6-15% of the weight of algal powder), which is a very rare natural blue pigment and nutrient, and has unique medicinal efficacy and fluorescent properties....

Claims

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Application Information

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IPC IPC(8): A23L2/38A23L2/52A23L2/66A23L33/17A23L33/10A23J1/00
CPCA23J1/009A23L2/38A23L2/52A23L2/66A23L33/10A23L33/17A23V2002/00A23V2200/30
Inventor 梁长利段敏静任嗣利郑祖凤许宝泉刘娟黄海威张卿洋张臻宇
Owner JIANGXI UNIV OF SCI & TECH
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