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A liquid biopsy detection method for circulating tumor cells

A tumor cell and liquid biopsy technology, applied in measuring devices, individual particle analysis, instruments, etc., can solve problems such as mutual interference, inability of immune magnetic beads to completely remove white blood cells, difficulty in culturing circulating tumor cells, etc., and achieve the effect of improving specificity

Active Publication Date: 2018-03-02
厦门骁科码生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The purpose of the present invention is to solve the above problems, that is, immunomagnetic beads cannot completely remove white blood cells; the two methods of antibody immunofluorescence staining and chromosome fluorescence in situ hybridization interfere with each other; the detection of trace cancer cells in a large number of blood cells is not sensitive, and after enrichment However, it provides a liquid biopsy monitoring method for circulating tumor cells

Method used

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  • A liquid biopsy detection method for circulating tumor cells
  • A liquid biopsy detection method for circulating tumor cells
  • A liquid biopsy detection method for circulating tumor cells

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Intelligent Quantitative FISH Hybrid Diagnosis Platform for Circulating Tumor Cells

[0040] Circulating tumor cells were enriched and counted. Tracking dynamic observation and studying the relationship between changes in the number of circulating tumor cells and clinical manifestations, clinical diagnosis, and clinical treatment is one of the most valuable projects in the field of liquid biopsy, but it is difficult to successfully obtain data. A series of dynamically changing CTC counts in a particular patient can be used to assess the tumor burden of that case, which is a perfect complement to the CTC assessment of tumor size at this point. It can also evaluate the patient's response to treatment, and it is also one of the best or most sensitive indicators for judging recurrence.

[0041] Based on the above situation, this embodiment uses the "Yiyi Enrichment Method" of the present invention, combined with the intelligent liquid biopsy system of the present invention...

Embodiment 2

[0081] Example 2 Circulating tumor cell solution FISH hybridization (FISH-IS) double staining diagnostic platform

[0082] Collect blood sample · Patient: 7.5mL——Remove red blood cells by non-hypotonic lysis method—Immune magnetic bead binding, remove white blood cells——FISH-IS (FISH in suspension) specific hybridization——Immunity method based on antigen-antibody binding Marked to form a double stain. Manual acquisition by microscopic observation, or automatic image acquisition with fully automated scanning fluorescence microscopy – bulk image analysis.

[0083] This embodiment introduces a new concept of completing FlSH in solution (ie FISH-IS), which is beneficial to obtain the best results without mutual interference between FlSH and immunohistochemical staining.

Embodiment 3

[0085] Solution FISH hybridization (FISH-IS)-based, modified BD FACS Melody circulating tumor cell detection and capture platform Collect blood samples Patient: 7.5mL——Non-hypotonic lysis method to remove red blood cells—Immunomagnetic bead binding, white blood cell removal— - FISH-IS (FISH in suspension) specific hybridization - flow cytometry analysis (flowcytometry). Flow cytometry analysis technology is through the rapid determination of the characteristics of individual cells combined with advanced computer systems, so that a large number of cells can be qualitatively and quantitatively determined in a short period of time, and even specific target CTCs can be captured.

[0086] Flow cytometry analysis (flowcytometry). Flow cytometry analysis technology is through the rapid determination of the characteristics of a single cell combined with advanced computer systems, so that a large number of cells can be qualitatively and quantitatively determined in a short period of ti...

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Abstract

The invention relates to a liquid biopsy detection method, particularly a liquid biopsy detection method for circulating tumor cells. The method includes enriching circulating tumor cells in a sample;separating and identifying the circulating tumor cells; and quantitatively counting the circulating tumor cells in unit volume. The intelligent liquid biopsy method combines the newest computer science and biological techniques, and innovatively provides a circulating tumor cell quantitative detection method, a circulating tumor cell separating method and a circulating tumor cell in-vitro culturemethod. The liquid biopsy detection method can achieve dynamic monitoring of tumor traits, tumor treatment and prognosis evaluation, tumor recurrence monitoring, and the like, and therefore the liquid biopsy detection method will greatly promote developments of individualized precise treatment in China.

Description

technical field [0001] The invention belongs to the field of molecular cell biology, and specifically relates to a detection method for enriching, identifying and quantifying circulating tumor cells per unit volume from human or animal biological fluid. Background technique [0002] The detection methods of circulating tumor cells and circulating tumor DNA (ctDNA) in blood are collectively referred to as liquid biopsy. Liquid biopsy has the advantages of quickness, simplicity, non-invasiveness, sufficient sample size, and continuous disease monitoring, so it has become a hot spot in the research of individualized precision therapy. [0003] Circulating tumor cells (CTCs) are tumor cells with special characteristics that fall off from solid tumors and enter the peripheral blood circulation. Evaluation of progress, curative effect and prognosis, and monitoring of tumor recurrence are of extremely important clinical significance. The clinical significance of CTC has been clea...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/531G01N21/64G01N15/14
CPCG01N15/14G01N21/6486G01N33/531G01N33/574
Inventor 贺红刘剑飞洪丰
Owner 厦门骁科码生物科技有限公司
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