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Treatment of bacterial infections in aquaculture

A technology of bacteria and vibrio, applied in antibacterial drugs, anti-infective drugs, medical raw materials derived from viruses/phages, etc., can solve short-term problems

Active Publication Date: 2018-02-27
FIXED PHAGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is well known that bacteriophages can only survive for relatively short periods of time in their natural environment, i.e. in water

Method used

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  • Treatment of bacterial infections in aquaculture
  • Treatment of bacterial infections in aquaculture
  • Treatment of bacterial infections in aquaculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] We tested phage covalently attached to plastic (nylon) and carbohydrate (cellulose) particles to demonstrate the feasibility of applying the compositions of the invention in aquaculture applications.

[0107] First, it was determined whether phage immobilized on cellulose powder survived longer than free phage in seawater and freshwater. figure 1 Survival rates of immobilized and non-immobilized phage (Φlin24) in seawater are shown. Phage immobilized on cellulose powder (Φlin24) survived significantly longer than non-immobilized phage in seawater (P≤0.001).

[0108] process

[0109] Media and methods

[0110] Table 1 - All media were prepared and methods followed appropriate standard operating procedures (SOPs).

[0111] Table 1

[0112]

[0113] "Agar 50" SOP

[0114] ●Weigh the required amount of powder

[0115] ●Add the powder to an empty bottle of sufficient volume

[0116] ●Add distilled water to the required volume

[0117] • Loosen the lid, which is se...

Embodiment 2

[0245] figure 2 The data presented demonstrate that immobilized phage are more resistant to UV irradiation than dissociated phage.

Embodiment 3

[0247] Figure 3 to Figure 6 Stability under various conditions including storage conditions for preparations of covalently linked phage is shown.

[0248] image 3 The storage stability of Pectinobacillus monophage immobilized on cellulose is shown. In this example, preparations were stored in liquid (PBS buffer) in single-use aliquots at 4°C.

[0249] Figure 4 The storage stability of Pectinobacillus monophage immobilized on copolymer beads is shown. with the above image 3 In contrast, the copolymer beads were stored in single-use aliquots at 4°C, but under dry conditions.

[0250] Figure 5 Shown are the relative viability (ie stability) of freed and immobilized phage when exposed to stress conditions. The stress conditions used were as follows:

[0251] 1. Humidity——4℃, 4 weeks

[0252] 2. Drying - 4°C, 4 weeks

[0253] 3. Ultraviolet radiation, 30 seconds

[0254] 4. 85°C, 1 minute

[0255] Image 6 The storage stability of freed and immobilized phage store...

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Abstract

A composition comprises bacteriophage covalently attached to an edible particle and is for use in treating bacterial infection in fish or crustaceans. Infections in fish or crustaceans caused by Vibrio, Aeromonas, Yersinia, Moritella, Rickettsia, Piscirickettsia, Lactococcus, Pseudomonas, Flavobacterium or Photobacterium bacteria species can be treated. Bacteria infected with a lysogenic bacteriophage are used for treating disease of fish or crustaceans caused by similar infections by bacteria carrying lysogenic bacteriophage that express a toxin gene.

Description

technical field [0001] The present invention relates to the treatment of bacterial infections in aquaculture, typically cultured shrimp, prawns and fish. In particular, the present invention relates specifically to compositions and methods for reducing or preventing infections and / or treating existing infections in such aquaculture. technical background [0002] Phages are the most numerous form of life on Earth. Bacteriophages exist wherever there is an environment for bacterial growth. Phages have been detected in ground and surface water, soil, food (eg, pickles, wine), sewage, and sludge. They can also be isolated from humans and animals, eg from feces, urine, saliva, saliva, rumen and serum. Phages are able to penetrate various organs and tissues, including the central nervous system, and are part of the gut microbiota along with their bacterial hosts. 10–80% of total bacterial mortality in aquatic ecosystems can be attributed to phages, and phages are an important ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/76A61K47/54A61K47/64A23K20/195A61P31/04
CPCA23K20/195A61K35/76A23K50/80A61P31/04Y02A40/81A61P31/00A23K20/163A61K47/61A61K47/62A61K47/6921A61K9/0053C12N7/00C12N2795/00032
Inventor 迈克尔·马泰
Owner FIXED PHAGE
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