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Rapid nucleic acid amplification system

A nucleic acid and rapid technology, applied in the fields of molecular biology and molecular diagnosis, can solve difficult and time-consuming problems, and achieve the effects of good repeatability, accurate positioning and fast heat balance

Pending Publication Date: 2018-02-27
XIAN TIANLONG SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a rapid nucleic acid amplification system, so that the nucleic acid amplification reaction solution in the reaction consumables can be transferred between different temperature ranges to achieve rapid nucleic acid amplification, so as to overcome the time-consuming or difficult to achieve in the existing nucleic acid amplification system. technical problem

Method used

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Examples

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Embodiment 1

[0037] The following will combine figure 1 , figure 2 , image 3 , to illustrate a specific embodiment of the present invention, the rapid nucleic acid amplification system shown in this example can be loaded with a disc-shaped reaction consumable, and adopts the mode of partial air bath to realize the rapid amplification of multiple nucleic acid samples at the same time .

[0038] Such as figure 1 As shown, the disc-shaped reaction consumables 21 in this embodiment include a rotating shaft card slot 22 and six PCR units 23, each PCR unit 23 includes a sample injection port 24, and a PCR premix chamber prepackaged with dry powder reagents 25 and three PCR reaction chambers 26, the three PCR reaction chambers 26 constitute a PCR reaction area 27; the sample loading port 24 communicates with the PCR premix chamber 25 through the sample flow channel 28; the PCR premix chamber 25 passes through the liquid The flow channel 29 communicates with three PCR reaction chambers 26 . ...

Embodiment 2

[0042] The following will combine Figure 4 , Figure 5 , Figure 6 , to illustrate a specific embodiment of the present invention, the rapid nucleic acid amplification system shown in this example can be loaded with six fan-shaped reaction consumables, and uses a local air bath to simultaneously achieve rapid amplification of multiple nucleic acid samples.

[0043] Such as Figure 4 As shown, the fan-shaped reaction consumables 31 in this embodiment include two rotating shaft card slots 32 and a PCR unit 33, and the PCR unit 33 includes a sample injection port 34, a PCR premix chamber 35 pre-packaged with dry powder reagents, and three PCR reaction chamber 36, three PCR reaction chambers 36 have constituted a PCR reaction area 37; The sample loading port 34 is connected with the PCR premix chamber 35 through the sample flow channel 38, and the PCR premix chamber 35 is connected with the PCR premix chamber 39 through the liquid separation flow channel 39. The PCR reaction c...

Embodiment 3

[0047] The following will combine Figure 7 , Figure 8 , to illustrate a specific embodiment of the rotary amplification device of the present invention. The rapid nucleic acid amplification system shown in this example adopts a temperature control module to realize rapid amplification of single or multiple nucleic acid samples.

[0048] Such as Figure 7-8 As shown, the rotary amplification device in this embodiment includes a turntable (not shown), a rotating motor 411 and two PCR temperature zone control units: the first PCR temperature zone control unit includes an upper temperature control module 12a 1 And lower temperature control module 12a 2 , the second PCR temperature zone control unit includes an upper temperature control module 12b 1 and lower temperature control module 12b 2 , Two sets of upper and lower temperature control modules are relatively installed on the upper and lower parts of the reaction consumables to control the same temperature. The two group...

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Abstract

The invention provides a rapid nucleic acid amplification system. Transfer of a nucleic acid amplification reaction solution in a reaction consumable among different temperature zones is performed bya rotating mode and rapid amplification of nucleic acid is realized; the technical problem that an existing nucleic acid amplification system is long in consumed time or the reaction consumable is difficult to realize is solved. The system provided by the invention comprises a disk-shaped or sector reaction consumable, a temperature control module and a rotary supporting assembly. A PCR (Polymerase Chain Reaction) reaction area close to the edge is arranged on the reaction consumable; the temperature control module comprises two or more groups of circumferentially-distributed PCR temperature zone control units and is used for controlling the temperature of the PCR reaction area on the reaction consumable. The reaction consumable is arranged on a supporting component of the rotary supporting assembly; the PCR reaction area is controlled by a rotary motor to transfer between different temperature zone control units and the rapid amplification of the nucleic acid is realized.

Description

technical field [0001] The invention relates to the fields of molecular biology and molecular diagnosis, in particular to a system for rapidly realizing nucleic acid amplification. Background technique [0002] Nucleic acid detection is a basic experimental method in molecular biology. Molecular diagnosis is a technology that uses molecular biology methods to detect exogenous or self-genetic material "nucleic acid" and its expression level in patients' bodies or body fluids to make a diagnosis. It is one of the important frontier areas of contemporary medical development. The basis of molecular diagnosis is the detection and analysis of target nucleic acids. Molecular diagnostic technologies mainly include real-time fluorescence quantitative PCR, fluorescence in situ hybridization (FISH), gene sequencing and gene chips, etc. Molecular diagnostic techniques basically include three steps: nucleic acid extraction and purification, nucleic acid amplification and detection. Nuc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/34
CPCB01L3/5027B01L7/5255B01L2300/18B01L2300/1805B01L2200/10
Inventor 彭年才李明苗保刚李政田春李红东孙瑶
Owner XIAN TIANLONG SCI & TECH
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