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A composite nanoparticle for three-dimensional fluorescence imaging of vascular network, its preparation method and application

A composite nanoparticle and network three-dimensional technology, which can be used in preparations, chemical instruments and methods, pharmaceutical formulations and other directions for in vivo experiments, can solve the problems of easy leakage of fluorescent materials, easy blockage of blood vessels, cost, complicated preparation methods, etc. Less interference from bleaching and background fluorescence, simple and easy preparation method, and good water dispersibility

Active Publication Date: 2020-06-12
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at the problems of poor fluorescence stability, easy leakage of fluorescent materials, high price, and complicated preparation methods in the prior art, the inventors conducted research and proposed a new type of composite nanoparticle
The composite nanoparticles designed in the present invention can be uniformly dispersed and fixed in the blood vessels. When used for fluorescence imaging, the brightness is high, the dyeing is uniform, the cost is low, and multi-channel imaging can be realized. It can achieve high-resolution three-dimensional imaging of the fine network structure of blood vessels due to many problems such as uniformity, easy blockage of blood vessels, and high cost.

Method used

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  • A composite nanoparticle for three-dimensional fluorescence imaging of vascular network, its preparation method and application
  • A composite nanoparticle for three-dimensional fluorescence imaging of vascular network, its preparation method and application
  • A composite nanoparticle for three-dimensional fluorescence imaging of vascular network, its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0030] 1. Preparation of polystyrene nanoparticles modified with surface amino groups (taking polystyrene nanoparticles with an average particle size of 150nm as an example)

[0031] (1) 0.025g of sodium lauryl sulfate and 0.2g of sodium bicarbonate are dissolved in 250ml of ultrapure water, heated under vigorous stirring to completely dissolve, add 15g of polystyrene, continue at room temperature With stirring, a saturated solution containing 10 g of potassium persulfate was added to the system. Under the protection of argon atmosphere, react at 70°C for 10 hours. After the reaction, the supernatant was removed by centrifugation, washed with ultrapure water and absolute ethanol three times each, dried and stored at room temperature.

[0032] (2) Weigh 1.0g of the material prepared in the above step (1), make it uniformly dispersed in ultrapure water, and add 25g of concentrated HNO dropwise to the system under stirring 3 / Concentrated H 2 SO 4 The mixed solution (volume ratio is ...

Embodiment 2

[0044] 1. Preparation of polystyrene nanoparticles modified with surface amino groups (taking polystyrene nanoparticles with an average particle size of 150nm as an example)

[0045] (1) 0.025g of sodium lauryl sulfate and 0.2g of sodium bicarbonate are dissolved in 250ml of ultrapure water, heated under vigorous stirring to completely dissolve, add 12.5g of polystyrene, continue at room temperature Under stirring, a saturated solution containing 10 g of potassium persulfate was added to the system. Under the protection of argon atmosphere, react at 70°C for 10 hours. After the reaction, the supernatant was removed by centrifugation, washed with ultrapure water and absolute ethanol three times each, dried and stored at room temperature.

[0046] (2) Weigh 1.0g of the material prepared in the above step (1), make it uniformly dispersed in ultrapure water, and add 25g of concentrated HNO dropwise to the system under stirring 3 / Concentrated H 2 SO 4 The mixed solution (volume ratio ...

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Abstract

The invention discloses a composite nano-particle for three-dimensional fluorescence imaging of a blood vessel network, and a preparation method thereof and an application thereof. The composite nano-particle includes 1-30 wt% of a fluorescent material used as a core and 70-99 wt% of an amino-modified polymer used as a shell layer. The fluorescent material has the advantages of controllable dimensions, good water dispersibility, high light stability, and simple and easy preparation process. The prepared composite nano-particle has the advantages of high fluorescence brightness, uniformity in dyeing, low interference of photobleaching and background fluorescence, low toxicity and low cost when used for the blood vessel imaging, can accurately mark the fine structure of the blood vessel network, and has potential practical values in in-vivo imaging, medical diagnosis, disease therapy and other important fields.

Description

Technical field [0001] The invention relates to the field of biomedicine, in particular to a composite nanoparticle for three-dimensional fluorescent imaging of blood vessel networks, a preparation method and application thereof. Background technique [0002] In recent years, fluorescence imaging technology has received high attention in the field of biomedicine as a kind of efficient visualization research method, and has shown broad application prospects in aspects such as gene expression, biological information transmission, and real-time monitoring of physiological processes in vivo. Compared with traditional imaging technology, fluorescence imaging technology has significant advantages such as high sensitivity, less biological damage, high imaging resolution, and real-time monitoring. [0003] The use of fluorescence technology for blood vessel imaging is extremely important in biological science research and disease diagnosis. Three-dimensional reconstruction of the fine net...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02A61K49/00
CPCA61K49/0017A61K49/0093C09K11/025
Inventor 张浩力曹晶晶龚晓婷张胜祥成晓峰谢文广
Owner LANZHOU UNIVERSITY
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