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Probiotics microecological preparation and preparation method and application thereof

A probiotic micro-ecological and micro-ecological technology, applied in the field of micro-ecological preparations, can solve the problems of tediousness, mutual interference of strains, and difficulty in meeting treatment needs.

Active Publication Date: 2018-02-16
山东宝来利来生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many similar products of probiotics on the market, but there are still the following major problems: (1) the synergistic effect between the strains in the product is not good, and there is mutual interference or mutual antagonism between the strains, which is One of the important factors affecting the quality of probiotics; (2) the content of live bacteria is low, and studies have shown that if the concentration of a kind of bacterial agent in the cecum content is lower than 10 7 1 / g, the enzymes and metabolites produced by the bacterial agent are not enough to affect the host, and it is difficult to meet the needs of treatment; (3) There are many types of microbial strains in the product, but there is no shortage of suffocating, and the functions of the strains are not efficient, the metabolites are not rich enough, and the probiotic effect is not enough. Strong; (4) The preparation process is complex and cumbersome, it is difficult to ensure that all strains grow and reproduce well, and the product consistency is poor
Due to these quality problems of probiotics, it affects its application effect on animals and limits the wide application of probiotics

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0067] The preparation of embodiment 1 bacterial strain preparation

[0068] 1. Lactococcus lactis CAMT22361

[0069] (1) Take Lactococcus lactis CAMT22361, activate and cultivate it at 37°C for 48h, then expand it statically at 37°C for 60h, and stir the culture medium for 10min every 12h during this period to obtain a liquid preparation of Lactococcus lactis with a viable count of 6.2×10 11 CFU / mL;

[0070] Among them, the medium components of activation culture are: 1% peptone, 1% beef extract, 0.5% yeast extract, 2% glucose, 0.2% dipotassium hydrogen phosphate, 0.5% sodium acetate, 0.2% triammonium citrate, 0.058% Magnesium sulfate heptahydrate, 0.025% manganese sulfate monohydrate, 0.1% Tween 80, pH 6.2-6.4;

[0071] The medium components of expanded culture are: 2% soybean peptone, 1% beef extract, 0.5% yeast extract, 2% glucose, 0.5% sodium chloride, 0.1% dipotassium hydrogen phosphate, 0.03% magnesium sulfate, 0.01% manganese sulfate , the pH value is natural;

[0...

Embodiment 2

[0083] The preparation of embodiment 2 probiotic micro-ecological liquid preparation A

[0084] A kind of probiotic microecological liquid preparation A, its preparation method is as follows:

[0085] (1) Prepare Lactococcus lactis liquid preparation, Bacillus amyloliquefaciens liquid preparation, Rhodotorula gum liquid preparation respectively according to the method for embodiment 1;

[0086] (2) In parts by weight, mix 40 parts of the above-mentioned Lactococcus lactis liquid preparation, 30 parts of the Bacillus amyloliquefaciens liquid preparation, and 30 parts of the Rhodotorula gum liquid preparation to obtain the probiotic microecological liquid preparation A, and the total number of live bacteria is about 2.6×10 11 CFU / mL.

Embodiment 3

[0087] The preparation of embodiment 3 probiotic micro-ecological liquid preparation B

[0088] A kind of probiotic microecological liquid preparation B, its preparation method is as follows:

[0089] (1) Take Lactococcus lactis CAMT22361, activate and cultivate it at 36°C for 36 hours, then expand it at 33°C for 48 hours, and stir the medium for 10 minutes every 12 hours during this period to obtain a liquid preparation of Lactococcus lactis with a viable count of 1.4×10 11 CFU / mL;

[0090] (2) Take Bacillus amyloliquefaciens ZJHD3-06, activate and cultivate it at 36°C for 24h, then expand and cultivate it at 34°C and 130r / min for 48h, to obtain the liquid preparation of Bacillus amyloliquefaciens, the number of viable bacteria is 2.6×10 9 CFU / mL;

[0091] (3) Take Rhodotorula japonicum ZTHY2, activate and cultivate at 28°C for 48h, and then expand and cultivate at 31°C and 130r / min for 60h to obtain the liquid preparation of Rhodotorula japonicus, the number of viable bact...

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Abstract

The invention discloses a probiotics microecological preparation and a preparation method and an application thereof. The probiotics microecological preparation comprises lactococcus lactis, bacillusamyloliquefaciens and rhodotorula mucilaginosa. The preparation method comprises the following steps: S1, preparing liquid preparations of strains; mixing the liquid preparations of the strains with bran in a weight ratio of 1: (4-11), airing the mixture at 40-60 DEG C, and crushing the mixture to obtain the solid preparations of the strains; and S2, preparation of the probiotics microecological preparation: uniformly mixing the liquid preparations of the strains to obtain probiotics microecological liquid preparations; and uniformly mixing the probiotics microecological liquid preparations toobtain the probiotics microecological preparation. The microecological preparation prepared by the invention is remarkable in synergic effect. The probiotics in the preparation are high in activity and rich in metabolite, the production performance of livestock in different stages can be improved, and the probiotics microecological preparation can be used as a feed additive and a substituent of antibiotics.

Description

technical field [0001] The invention belongs to the technical field of microecological preparations, and more specifically relates to a probiotic microecological preparation and its preparation method and application. Background technique [0002] Microecological preparations are a new type of microbial additives that have risen rapidly at home and abroad in recent years. They have the advantages of regulating the balance of intestinal flora, antagonizing pathogenic bacteria, promoting animal growth, improving feed conversion rate and enhancing immune function. They have been widely used in livestock The poultry farming and feed industries have also become an ideal substitute for antibiotics in feed. [0003] At present, there are many similar products of probiotics on the market, but there are still the following major problems: (1) the synergistic effect between the strains in the product is not good, and there is mutual interference or mutual antagonism between the strain...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16A23K10/18C12R1/07C12R1/01C12R1/645
CPCA23K10/18C12N1/16C12N1/20
Inventor 徐春厚刘颖谢为天邝雪梅
Owner 山东宝来利来生物工程股份有限公司
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