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A strain of bacteria capable of degrading the insecticides buprofezin and bifenthrin and its production agent

A bifenthrin and insecticide technology, applied in the field of biological high-tech, can solve the problems of unfavorable industrial production, unstable degradation properties, easy loss of degradation genes, etc., achieve wide application potential and value, good removal effect, and protect people health effects

Active Publication Date: 2020-06-05
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The structures of buprofezin and bifenthrin are similar, and only Rhodococcus sp.YL-1 can degrade these two pesticides at the same time, but its degradation gene is easily lost, and its degradation properties are unstable, which is not conducive to large-scale industrial production , which restricts the use of this bacteria to repair buprofezin and bifenthrin residues in the environment

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  • A strain of bacteria capable of degrading the insecticides buprofezin and bifenthrin and its production agent
  • A strain of bacteria capable of degrading the insecticides buprofezin and bifenthrin and its production agent
  • A strain of bacteria capable of degrading the insecticides buprofezin and bifenthrin and its production agent

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Embodiment 1

[0025] The isolation and identification of embodiment 1 bacterial strain

[0026] The invention provides a stable and highly efficient bacterial strain for degrading buprofezin and bifenthrin and the bacterial agent D-6 produced therefrom, which is isolated from a soil polluted by buprofezin for a long time in Jiangsu Province. The screening method of bacterial strains is as follows: take 5g soil sample and add to 100mL containing 50mg·L -1 In the shaking flask of the basal salt medium of buprofezin (hereinafter referred to as BMM), place 30 ℃, 150rpm in the shaker culture, after 5 days, transfer to the shaking flask containing fresh BMM with 5% inoculation amount, carry out continuously Three enrichment cultures. Dilute the enrichment solution from the last passage and spread it on the BMM solid medium. After culturing at 30°C for 5 days, pick a single colony that formed a transparent circle and culture it in a 4 mL LB test tube. After the bacterial cells grow thick, a part...

Embodiment 2

[0028] Embodiment 2 laboratory degradation experiment

[0029] 2.1 Preparation of seed solution

[0030] Pick a single colony that has been activated on the plate, inoculate it in LB medium, and culture it in a shaker at 30°C and 150rpm until OD 600≈1.0. Collect the cells by centrifugation (4°C, 6000rpm for 5min), wash twice with basal salt medium and resuspend the cells to OD 600 ≈2.0, this is the seed liquid.

[0031] 2.2 Degradation of buprofezin by strain D-6

[0032] The seed liquid of strain D-6 was inoculated into BMM with an inoculum amount of 2%, and placed in a shaker at 30° C. at 150 rpm for 48 hours. Take 3 mL of culture solution every 8 hours, detect and calculate the concentration and degradation rate of remaining buprofezin in the culture medium by HPLC, and at the same time, dilute the regularly sampled culture solution and apply LB solid medium, calculate the growth of bacteria, and draw the strain D -6 to the growth degradation curve of buprofezin (as ...

Embodiment 3

[0041] Embodiment 3 degradation stability experiment

[0042] Since the reported strain Rhodococcus qingshengii YL-1 (CCTCC AB 2017132) has unstable degradation properties of buprofezin, it is necessary to evaluate the stability of the degradation properties of strain D-6. Pick freshly activated single colonies of strain D-6 and strain YL-1 on the BMM plate and form a transparent circle, inoculate them into test tubes containing 4 mL of LB medium, and culture them in a shaker at 30°C and 150 rpm until OD 600 ≈1.0. Transfer this bacterial solution to another test tube containing 4mL LB medium with 1% inoculum, and cultivate to OD under the same culture conditions. 600 ≈1.0, this is the first passage. Dilute the bacterial solution of the first passage to an appropriate multiple (10 -4 ~10 -6 ) after coating the BMM flat plate, and after the colony grows out, count the percentage of the colony number that cannot form a transparent circle to the total number of colonies. Per...

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Abstract

The invention belongs to the field of bioremediation of environmental pollution, and discloses a bacterium capable of stably degrading the insecticides buprofezin and bifenthrin and the bacterial agent produced by it, which was preserved in the China Typical Culture Collection Center on September 25, 2017 , the strain preservation number is CCTCC NO.M 2017537. The strain was Gram-positive strain D‑6, which was identified as Rhodococcus sp. The degrading strain D-6 described in the present invention can be used for the degradation of buprofezin and bifenthrin pesticides, and the use of this bacterial agent can reduce the residual amount of buprofezin in the soil by 95% in a short time % or more, reduce the residual amount of bifenthrin in the soil by more than 70% within a certain period of time, and its degradation properties are more stable than the reported degradation strain Rhodococcus sp. Azizone and bifenthrin residue contamination.

Description

technical field [0001] The invention discloses a strain of bacteria capable of stably degrading the insecticides buprofezin and bifenthrin and the bacteria agent produced in the biological high-tech field of the present invention. Background technique [0002] Chemical pesticides play an important role in the comprehensive management of crop diseases, insects, and weeds, which greatly promotes the development of modern agriculture, but at the same time it also brings serious environmental problems. First, the quality of agricultural products is reduced due to excessive pesticide residues, and exports are restricted. Second, pesticide residues can be enriched through the food chain, threatening human life and health. In addition, pesticide residues harm non-target organisms, indirectly changing the structure of the ecosystem and destroying its functions. [0003] Buprofezin is a high-efficiency and broad-spectrum triazine insecticide, which has a remarkable control effect on ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCA62D3/02A62D2101/04A62D2101/28C12N1/20C12N1/205C12R2001/01
Inventor 闫新陈雪婷纪俊宾洪青何健蒋建东
Owner NANJING AGRICULTURAL UNIVERSITY
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