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Method for establishing radix stellariae tissue culture systems

An establishment method and technology of Yin Bupleurum, applied in the establishment field of Yin Bupleurum tissue culture system, can solve the problems of reducing survival rate and seedling rate, low income and high cost, and achieve the improvement of reproduction speed and scale, high seed reproduction coefficient, Realize the effect of factory production

Inactive Publication Date: 2018-02-16
李正美
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When using seeds for propagation, the seeds have the problems of difficult germination and low germination rate, which greatly reduces the survival rate and seedling rate, and the cost is high and the income is low; Slow, not easy to survive, and also caused the problem of the seedling rate of Yin Bupleurum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Disinfection of explants: Rinse the collected stems of Bupleurum chinensis with clear water for 5 minutes and gently brush off the impurities on them with a brush. Disinfect them with 75% ethanol solution in an ultra-clean workbench for 5 seconds, and then use non-toxic Bacterial water was washed 6 times, and then rinsed with 5% Antifoam solution for 7 minutes, rinsed with sterile water 6 times, and then used sterile filter paper to absorb the water before use.

[0017] (2) Induction culture: Cut the stems of Silver Bupleurum sterilized in step (1) into about 2.0 cm long stems according to the paired buds as a unit, cut off the browned part of the bottom of the stems during the treatment, and inoculate into Bud induction culture was carried out in induction medium. After inoculation, they were first cultured in total darkness at 26°C for 5 days, and then placed in light for 10 hours a day with a light intensity of 2100lx, and placed at a culture temperature of 25°C ...

Embodiment 2

[0022] (1) Disinfection of explants: Rinse the collected stems of Bupleurum chinensis with clear water for 8 minutes and gently brush off the impurities on them with a brush. Disinfect them with 75% ethanol solution in an ultra-clean workbench for 16 seconds. Bacteria washed twice, then rinsed with 5% Antifoam solution for 4 minutes, rinsed twice with sterile water, and then blotted with sterile filter paper for later use.

[0023] (2) Induction culture: Cut the stems of Silver Bupleurum sterilized in step (1) into about 1.0 cm long stems according to the paired buds as a unit, cut off the browned part of the bottom of the stems during the treatment, and inoculate into Bud induction culture was carried out in induction medium. After inoculation, they were first cultured in total darkness at 26°C for 2 days, and then placed in light for 9 hours a day with a light intensity of 2400lx, and cultured at a temperature of 26°C until adventitious buds formed, and the induction rate wa...

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Abstract

The invention discloses a method for establishing radix stellariae tissue culture systems. Radix stellariae is a caryophyllaceae stem plant, the roots of the radix stellariae need to be dug out from August to October when the stems and leaves of the radix stellariae wither, rhizomes and fibrous roots and sediment on the roots need to be removed, and the radix stellariae needs to be dried in the sun. The medicinal properties of the radix stellariae include that the cylindrical roots of the radix stellariae occasionally have lateral roots, the surfaces of the roots are light yellow or yellowishwhite, the root tips have a plurality of tuberculate stem residues, the roots are light and fragile and are easy to break, breaking faces are uneven and loose and have crack, and radial texture is formed by xylem bundles and rays in a yellow-white alternative manner. The radix stellariae has light smell and slightly sweet taste. The radix stellariae has uniform root lengths and light yellow cortex. The radix stellariae mainly contains saponin and cyclitols such as di-pinitol. Effects of clearing deficient heat can be realized by the radix stellariae. The radix stellariae can be used for feverdue to yin deficiency, fever due to infantile malnutrition and the like. The method has the advantages that stems with buds are used as explants, radix stellariae tissue culture regenerated plants canbe successfully obtained by the aid of steps of induction, proliferation, rooting, seedling exercising, transplanting and the like, and the systematic radix stellariae tissue culture plant regeneration systems can be established by the aid of the method; the radix stellariae tissue culture systems are high in reproduction coefficient, seedling rate and earning.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for establishing a silver bupleurum tissue culture system. Background technique [0002] Yinbupleurum is a stem of Dianthus, mainly produced in Ningxia, Shaanxi, Inner Mongolia and other places. In addition, it is also produced in Northeast China. When the stems and leaves are withered from August to October, dig out the roots, remove the rhizomes and fibrous roots, silt, and dry them in the sun. Medicinal properties: the root is cylindrical, with occasional branches, 15-45cm long and 1-3cm in diameter. The surface is light yellow or yellowish white, with obvious longitudinal wrinkles, often twisting to the left, and sometimes root scars can be seen, in the shape of sunken brown dots; there are many verrucous protruding stem residues on the root head. Light and brittle, easy to break, the broken surface is uneven, loose, with cracks, xy...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 李正美
Owner 李正美
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