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Method for preparing two-dimensional nano-material by using silk fibroin stripping

A technology of two-dimensional nanomaterials and silk fibroin, which is applied in the field of nanomaterial preparation and biomedical application, can solve the time-consuming problems of two-dimensional nanomaterials, and achieve rapid and simple peeling preparation process, good dispersibility, and good biocompatibility sexual effect

Active Publication Date: 2018-02-09
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The present invention overcomes the time-consuming problems in the preparation of two-dimensional nanomaterials by exfoliating existing biopolymers, successfully and rapidly prepares graphene or black phosphorus two-dimensional nanomaterials with less than ten layers, and has important application value in the field of biomedicine

Method used

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  • Method for preparing two-dimensional nano-material by using silk fibroin stripping

Examples

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Effect test

Embodiment 1

[0016] Fabrication of graphene nanomaterials using silk fibroin exfoliation

[0017] The silk fibroin is degummed, dissolved, dialyzed and filtered to prepare a silk fibroin aqueous solution with a mass concentration of 5%. The silk fibroin aqueous solution is reacted with a diazonium salt to modify the tyrosine residue of the silk fibroin by carboxylation. It is then reacted with chloroacetic acid to carboxylate serine residues. After dialysis, a carboxylated silk fibroin solution with a mass concentration of 2% was prepared. Disperse 45g of graphite powder into 10ml of carboxylated silk fibroin solution, and then ultrasonicate for 2 hours at an ultrasonic power of 300W, then move the aqueous solution into a microtube and wash it by centrifugation to obtain a single layer of graphite dispersed in the aqueous solution ene two-dimensional nanomaterials. At the same time, the graphene two-dimensional nanomaterials were co-incubated with the cells, and after 24 hours of co-cult...

Embodiment 2

[0019] Fabrication of black phosphorus nanomaterials using silk fibroin exfoliation

[0020] The silk fibroin is degummed, dissolved, dialyzed and filtered to prepare a silk fibroin aqueous solution with a mass concentration of 16%. The silk fibroin aqueous solution is reacted with a diazonium salt to modify the tyrosine residue of the silk fibroin by carboxylation. It is then reacted with chloroacetic acid to carboxylate serine residues. After dialysis, a carboxylated silk fibroin solution with a mass concentration of 8% was prepared. Disperse 10g of black phosphorus powder into 200ml of carboxylated silk fibroin solution, and then ultrasonicate for 5 hours at an ultrasonic power of 50W. Black phosphorus two-dimensional nanomaterials. At the same time, the black phosphorus two-dimensional nanomaterials were co-incubated with the cells. After 24 hours of co-cultivation with the cells at a high black phosphorus concentration (500 μg / mL), the survival rate of the cells was sti...

Embodiment 3

[0022] Fabrication of black phosphorus nanomaterials using silk fibroin exfoliation

[0023] The silk fibroin is degummed, dissolved, dialyzed and filtered to prepare a silk fibroin aqueous solution with a mass concentration of 20%. The silk fibroin aqueous solution is reacted with a diazonium salt to carry out carboxylation modification on the tyrosine residue of the silk fibroin. It is then reacted with chloroacetic acid to carboxylate serine residues. After dialysis, a carboxylated silk fibroin solution with a mass concentration of 15% was prepared. Disperse 80g of black phosphorus powder into 800ml of carboxylated silk fibroin solution, then vibrate for 1 hour at a shaking power of 300W, then move the aqueous solution into a microtube, and wash it by centrifugation to obtain a single layer of silk fibroin dispersed in the aqueous solution. Black phosphorus two-dimensional nanomaterials. At the same time, the black phosphorus two-dimensional nanomaterials were co-incubate...

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Abstract

The present invention provides a simple method for preparing a two-dimensional nano-material, which uses natural and abundant-content silk fibroin as a stripping agent. According to the invention, ultrasonic wave (or oscillation) stripping is carried out on layered materials such as graphite or black phosphorus to achieve large-scale preparation of the graphene or black phosphorus two-dimensionalnano-material with a small layer structure, and the nano material has excellent biocompatibility and dispersion performance. The method employs physical stripping, and the prepared two-dimensional nano-material has excellent crystallinity. The preparation method of the invention has the advantages of simple process, low cost, no requirement of complicated equipment, and environmental protection.

Description

technical field [0001] The invention belongs to the field of nanomaterial preparation and biomedical application, and specifically relates to the use of carboxylated silk fibroin solution as a stripping agent to prepare a two-dimensional nanomaterial with a single-layer or few-layer structure. The formed nanomaterial has excellent dispersibility and Biocompatibility has important application value in the field of biomedicine. Background technique [0002] Since graphene was first discovered by mechanical exfoliation in 2004, two-dimensional nanomaterials have attracted extensive attention and research. It is well known that the properties of two-dimensional nanomaterials are closely related to their thickness. Two-dimensional nanomaterials with a single-layer or few-layer structure present unique mechanical, optical, and electrical characteristics, and have become a hot research topic today. So far, in addition to graphene, many graphene-like materials have also received m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B32/19C01B25/00
CPCC01B25/003C01B2204/04C01P2004/04
Inventor 杨黄浩韦晶晶黄晓卫张雪亮白树猛
Owner FUZHOU UNIV
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