Creation method of paddy rice common nuclear sterile line and application
A common nuclear sterile line and nuclear sterile technology, applied in the field of rice breeding, can solve the problems of heavy workload and long time consumption
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[0039] Example 1 Creation of rice common male sterile line cyp703a3-a
[0040] 1. Obtain the male sterility gene sequence from the cloned genetic resources
[0041] The target rice male sterility gene CYP703A3 was obtained from GeneBank, and its gene sequence is shown in SEQ ID No.1; the selected target sequence is shown in SEQ ID NO.2.
[0042] 2. Reducing the expression level of CYP703A3 protein in rice varieties by means of CRISPR-Cas9
[0043] Using the common fertile material (genotype MsMs) indica rice 9311 as the recipient, through the mediation of Agrobacterium EHA105-BGKO5, using the conventional rice transgenic operation method, in the transgenic population, select a single plant with a phenotype of sterility, First-generation sequencing was performed on the target sequence, and a sterile single plant cyp703a3-a (genotype msms) was obtained.
[0044] In order to apply the CYP703A3 protein, the CYP703A3 gene CRISPR-Cas9 vector CYP703A3-BGKO5 (vector from Baige Compa...
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