Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Creation method of paddy rice common nuclear sterile line and application

A common nuclear sterile line and nuclear sterile technology, applied in the field of rice breeding, can solve the problems of heavy workload and long time consumption

Active Publication Date: 2018-02-09
HUNAN HYBRID RICE RES CENT
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problem encountered in the application is: the common GMS resources required for the construction of the "third-generation hybrid rice" system require the common GMS genes that have been genetically cloned (that is, genes with relatively thorough basic research) at the genome level Changing the expression of genes at a fixed point, thereby changing the target traits. The materials derived from these mutants are usually not the materials that breeders are most looking forward to using directly (materials for basic research are not the best and latest materials in production), so these materials should be For production, it must be transferred through backcrossing, etc., which is a time-consuming and heavy workload.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Creation method of paddy rice common nuclear sterile line and application
  • Creation method of paddy rice common nuclear sterile line and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Creation of rice common male sterile line cyp703a3-a

[0040] 1. Obtain the male sterility gene sequence from the cloned genetic resources

[0041] The target rice male sterility gene CYP703A3 was obtained from GeneBank, and its gene sequence is shown in SEQ ID No.1; the selected target sequence is shown in SEQ ID NO.2.

[0042] 2. Reducing the expression level of CYP703A3 protein in rice varieties by means of CRISPR-Cas9

[0043] Using the common fertile material (genotype MsMs) indica rice 9311 as the recipient, through the mediation of Agrobacterium EHA105-BGKO5, using the conventional rice transgenic operation method, in the transgenic population, select a single plant with a phenotype of sterility, First-generation sequencing was performed on the target sequence, and a sterile single plant cyp703a3-a (genotype msms) was obtained.

[0044] In order to apply the CYP703A3 protein, the CYP703A3 gene CRISPR-Cas9 vector CYP703A3-BGKO5 (vector from Baige Compa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a creation method of a paddy rice common nuclear sterile line and application. The creation method is characterized a common fertile breeding material is used to create the sterile line, the sterile line is hybridized with an intermediate male parent material, hybrid seeds are sorted according to sorting gene expression characters to obtain a large number of sterile-line seeds which do not contain transgenic components, and the reproduction of the common nuclear sterile line is achieved. The common nuclear sterile line and a restoring line are used to produce the hybridseeds, the breeding procedure is simple, a large amount of backcross transformation time can be saved, the sterile characters of the common nuclear sterile line are fast imported into the fertile material, and the breeding cycle is shortened greatly.

Description

technical field [0001] The invention belongs to the technical field of rice breeding, and in particular relates to a creation method and application of a common nuclear male sterile line of rice. Background technique [0002] Rice is an important food crop in the world. About 50% of the world's population uses rice as a staple food. Rice is also a raw material for processing many foods. The "three-line method" and "two-line method" are the main ways to utilize rice hybrid vigor at present, but the problem with the "three-line method" is that it is limited by the strict restoration relationship, and the parental resources that can be used are limited; the "two-line method" There is a greater risk in the safety of seed production, which limits the potential of these two approaches to fully exploit rice heterosis. [0003] Common GMS has the characteristics of a wide range of sources, no restriction of restoration relationship, complete abortion and no environmental influence ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04C12N9/22C12N15/8213C12N15/8287C12N15/829
Inventor 李莉宋书锋李懿星王天抗李新奇袁定阳邝翡婷袁隆平
Owner HUNAN HYBRID RICE RES CENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products