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Sterile cavity tissue separation method

A tissue separation and cavity technology, applied in the fields of botanical equipment and methods, horticulture, plant cultivation, etc., can solve the problems of less mycelium, contamination of miscellaneous bacteria, difficult operation, etc. The effect of avoiding bacterial contamination

Inactive Publication Date: 2018-02-09
翔天科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But for colloidal bacteria, such as black fungus, white fungus, etc., due to the thin ear pieces, the operation is difficult, it is difficult to obtain tissue pieces of sufficient size, and it is easy to be contaminated by bacteria
On the other hand, because there are many colloids and few hyphae in the fruiting body, it is more difficult to obtain pure strains from tissue separation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for isolating aseptic cavity tissue, which comprises the steps of:

[0023] (1) Place the medium in the bottle, 70% of the bottle is compacted medium, the rest of the bottle is empty, the inner wall of the bottle is clean, and the inside of the bottle is a sterile environment;

[0024] (2) The mycelium is in the bottle, and it is normal to germinate. During the process of germinating, sterile air is actively provided to the bottle, and at the same time exhaust gas is discharged from the exhaust gas outlet, and finally the primordia of the convex body is formed;

[0025] (3) Select culture objects that grow robustly and have no pollution as the isolation material, and take the internal tissue of the primordia for tissue isolation.

[0026] The sterile air is purified through an air filter, the primary air filter has a pore size of 0.3 μm, and the secondary air filter has a pore size of 0.01 μm, and the air filter is connected to an air compressor.

[0027] The ...

Embodiment 2

[0035] A method for isolating aseptic cavity tissue, which comprises the steps of:

[0036] (1) Place the culture medium in the bottle, the bottom 65% of the bottle is the compacted culture medium, the rest of the bottle is empty, the inner wall of the bottle is clean, and the inside of the bottle is a sterile environment;

[0037] (2) The mycelium is in the bottle, and it is normal to germinate. During the process of germinating, sterile air is actively provided to the bottle, and at the same time exhaust gas is discharged from the exhaust gas outlet, and finally the primordia of the convex body is formed;

[0038] (3) Select culture objects that grow robustly and have no pollution as the isolation material, and take the internal tissue of the primordia for tissue isolation.

[0039] The sterile air is purified through an air filter, the primary air filter has a pore size of 0.1 μm, the secondary air filter has a pore size of 0.01 μm, and the air filter is connected to an air...

Embodiment 3

[0048] A method for isolating aseptic cavity tissue, which comprises the steps of:

[0049] (1) Place the medium in the bottle, 75% of the bottle is compacted medium, the rest of the bottle is empty, the inner wall of the bottle is clean, and the inside of the bottle is a sterile environment;

[0050] (2) The mycelium is in the bottle, and it is normal to germinate. During the process of germinating, sterile air is actively provided to the bottle, and at the same time exhaust gas is discharged from the exhaust gas outlet, and finally the primordia of the convex body is formed;

[0051] (3) Select culture objects that grow robustly and have no pollution as the isolation material, and take the internal tissue of the primordia for tissue isolation.

[0052] The sterile air is purified through an air filter, the primary air filter has a pore size of 0.5 μm, and the secondary air filter has a pore size of 0.01 μm, and the air filter is connected to an air compressor.

[0053] The ...

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PUM

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Abstract

The invention relates to a sterile cavity tissue separation method which is that tissue separation is performed by taking a colloidal mushroom entity primordium. The sterile cavity tissue separation method comprises the following steps that 1, a culture medium is put in a bottle, 65%-75% of the inside of the bottle is the compacted culture medium, the residual part in the bottle is emptied out, the inner wall of the bottle is clean, and the inside of the bottle is a sterile environment; 2, a mycelium is in the bottle and normally grows and diffuses, sterile air is actively provided for the bottle in the growing and diffusing process, meanwhile waste gas is discharged out, and finally the primordium of a convex body is formed; 3, an uncontaminated culture object growing healthy and strong is selected as a separating material, and the internal organization of the primordium is taken for tissue separation. The separating method is simple, good in separating effect, free of infectious microbes and thorough in separation.

Description

technical field [0001] The invention relates to the technical field of edible fungi, in particular to a method for separating aseptic cavity tissue. Background technique [0002] The tissue isolation method of edible fungi usually refers to the method of obtaining fruiting body tissues under aseptic conditions and culturing them under suitable conditions to obtain pure strains. This is a necessary technique for strain rejuvenation. But for colloidal bacteria, such as black fungus, white fungus, etc., because the ear piece is thin, the operation is difficult, it is difficult to obtain a tissue block of sufficient size, and it is easy to be contaminated by bacteria. On the other hand, because there are many colloids and few hyphae in the fruiting bodies, it is more difficult to obtain pure strains through tissue separation. Contents of the invention [0003] In order to solve the above problems, the present invention provides a method for separating aseptic cavity tissue w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/40
Inventor 张金霞黄晨阳汪建党侯艳平肖俊培韩美玲王晶乔杰隋杰史振霞
Owner 翔天科技股份有限公司
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