Hemsleya amabilis tissue culture and later propagation method

Abstract

The invention belongs to the field of agricultural high technologies and specifically relates to a hemsleya amabilis tissue culture and lateral propagation method. The method comprises the following steps of initial medium preparation, explants sterilization and treatment, inoculation and culture, subculture medium preparation and inoculation, rooting culture, seedling hardening and transplantation. The method disclosed by the invention overcomes loss of effective ingredients cultured under a specific environment of a laboratory in the prior art and achieves artificial cultivation of wild varieties. Detection shows that dihydrocucurbitacin, cucurbitacin and hemsleya amabilis saponins all can be detected from the hemsleya amabilis propagated by the method disclosed by the invention, and contents of the dihydrocucurbitacin, the cucurbitacin and the hemsleya amabilis saponins are equivalent to that of the dihydrocucurbitacin, the cucurbitacin and the hemsleya amabilis saponins in wild hemsleya amabilis. The method disclosed by the invention improves a qualified seedling percentage of hemsleya amabilis seedlings, shortens a cultivation period, keeps effectiveness of the wild hemsleya amabilis and has an application prospect in the aspects of preserving, protecting, developing and utilizing wild hemsleya amabilis resources and the like.

Description

technical field [0001] The invention relates to a method for tissue culture, in particular to a method for tissue culture and later breeding of snow gall, belonging to the field of agricultural high-tech. Background technique [0002] Snow gall is a plant of the genus Snow gall of Cucurbitaceae. According to current knowledge, there are more than 20 species of this genus, mainly produced in Southwest China. It is bitter and cold in nature, less used in traditional medicine, and rich in wild resources. However, with the development of medicine, studies have found that snow gallbladder has the effects of clearing heat and detoxifying, reducing inflammation and relieving pain. Commonly used for gastric pain, ulcer disease, upper respiratory tract infection, bronchitis, pneumonia, bacillary dysentery, enteritis, urinary tract infection, sepsis and other infections. In recent years, Xuedansu Tablets, Xuedan Weichang Pills, Xuedansu Capsules, Xuedanjiedu Pills and other crude ex...

AI Technical Summary

Problems solved by technology

The tissue culture of Snow Gallbladder adopts artificially prepared culture medium. Under suitable temperature and environmental conditions, fast propagation induction is carried out to Snow Gallbladder, which can shorten the cultivation time, but the existing technology is easy to cause the snow gall seedlings to Asexual variation occurs, and the rapid propagation medium in the prior art has problems such as low tissue induction rate, poor quality of induced seedlings, many weak seedlings and low seedling proliferation efficiency, and low seedling survival rate after transplanting