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A method for efficiently extracting tetrodotoxin

A tetrodotoxin and high-efficiency technology, applied in organic chemistry and other directions, can solve the problems of expensive equipment, low efficiency, and low product purity, and achieve the effects of large-scale production, reduced risk, and high extraction efficiency.

Active Publication Date: 2020-03-17
MARINE FISHERIES RES INST OF ZHEJIANG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Although there are many methods for extracting and purifying tetrodotoxin in order to solve the above-mentioned, these methods more or less have certain defects, such as the high-efficiency liquid phase method with high output purity also has the problem of expensive equipment (high pressure liquid phase instrument) , Another example is the impregnation fractionation method, which has problems such as low efficiency and low product purity. A method for large-scale extraction and preparation of tetrodotoxin is provided. The method is simple and efficient, with high yield and good quality. -99%, directly meet the requirements of biological research and clinical application

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  • A method for efficiently extracting tetrodotoxin
  • A method for efficiently extracting tetrodotoxin
  • A method for efficiently extracting tetrodotoxin

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1: the extraction of tetrodotoxin

[0038] 1) Mash and homogenize 50kg puffer fish viscera, stir and soak in alcohol-water mixture containing acetic acid for 1 hour, centrifuge at 6200r / m, stir in a water bath, filter the precipitate, and remove the precipitate Repeat the method for more than 3 times, combine and filter the obtained filtrate, and concentrate; remove the fat contained in the filtrate with a degreasing liquid to obtain the yellowish venom; wherein the acetic acid content of the mixed solution is 1%, the alcohol content is 12%, and the balance It is water; the degreasing liquid is methanol solution of chloroform, and chloroform is 10%;

[0039] 2) Under low temperature conditions, the Weihuangchu venom is decolorized and purified with wooden activated carbon, and then the activated carbon is filtered out, and then the D201 macroporous adsorbent is further adsorbed and purified, and the D201 macroporous adsorbent is filtered out to obtain the pri...

Embodiment 2

[0044] Embodiment 2: the extraction of tetrodotoxin

[0045] 1) Mash and homogenize 50kg puffer fish viscera, stir and soak with alcohol-water mixture containing acetic acid for 2 hours, and centrifuge at a speed of 5800r / m, stir in a water bath, filter the precipitate, and remove the precipitate Repeat the method more than 2 times, combine and filter the obtained filtrate, and concentrate; remove the fat contained in the filtrate with a fat-removing liquid to obtain the yellowish venom; wherein the acetic acid content of the mixed solution is 0.8%, the alcohol content is 10%, and the balance It is water; the degreasing liquid is methanol solution of chloroform, and chloroform is 20%;

[0046] 2) Under low temperature conditions, the Weihuangchu venom is decolorized and purified with wooden activated carbon, and then the activated carbon is filtered out, and then further adsorbed and purified with D101 macroporous adsorbent, and the D101 macroporous adsorbent is filtered out t...

Embodiment 3

[0051] Embodiment 3: the extraction of tetrodotoxin

[0052] 1) Mash and homogenize 50kg puffer fish viscera, stir and soak with alcohol-water mixture containing acetic acid for 2 hours, centrifuge at 6200r / m, stir in a water bath, filter the precipitate, and remove the precipitate Repeat the method for more than 3 times, combine and filter the obtained filtrate, and concentrate; remove the fat contained in the filtrate with a fat-removing liquid to obtain the yellowish venom; wherein the acetic acid content of the mixed solution is 1%, the alcohol content is 10%, and the balance It is water; the degreasing liquid is methanol solution of chloroform, and chloroform is 15%;

[0053] 2) Under low temperature conditions, the Weihuangchu venom is decolorized and purified with wooden activated carbon, and then the activated carbon is filtered out, and then the D201 macroporous adsorbent is further adsorbed and purified, and the D201 macroporous adsorbent is filtered out to obtain th...

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Abstract

The invention relates to a method for massively and extremely efficiently extracting high-purity tetrodotoxin from viscera of globefishes, and aims at overcoming defects, in existing various methods for extracting and purifying tetrodotoxin, that the efficient liquid phase method with high product purity is high in cost of equipment (a high-pressure liquid phase instrument), and the dipping fractionation method is low in efficiency and low in product purity. The tetrodotoxin is applicable to drug rehabilitation, analgesia and pressure reduction drug preparations. The method is performed for massively extracting and preparing tetrodotoxin. The method is simple in processes, high in efficiency, high in yield, and high in quality; the product purity is up to 97-99% and directly meets the requirements of biological study and clinical application. Additionally, the invention aims at providing various preparations containing the tetrodotoxin prepared by the method.

Description

technical field [0001] The invention relates to the field of extraction of chemical substances, in particular to a large-scale and efficient method for extracting and producing tetrodotoxin from puffer fish viscera as raw materials. Its products can be used in drug detoxification, analgesic and antihypertensive pharmaceutical preparations. Background technique [0002] Tetrodotoxin (TTX) is a low molecular weight alkaloid extracted from the viscera of puffer fish. Due to its unique chemical structure, it is a specific blocker of sodium ion channels in cells. Therefore, it has an irreplaceable position as a biological reagent in the field of biological research. The pharmacological effects of tetrodotoxin in clinical medicine have been reported as early as the 1930s for the clinical intervention of alkaloid addiction. There are also continuous reports on analgesic treatment. Therefore, it plays a very important role both in the field of biological research and in the clin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D491/22
Inventor 张小军陈思曾军杰卢义博张勋
Owner MARINE FISHERIES RES INST OF ZHEJIANG
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