Targeting cell-penetrating peptide vector based on histidine and application

Abstract

The invention discloses a targeting cell-penetrating peptide vector based on histidine and an application. The amino acid sequence of the targeting cell-penetrating peptide vector based on histidine is REDV-YGRKKRRQRRR-PKKKRKV-Hm, wherein m ranges from 4 to 12. The targeting cell-penetrating peptide vector based on histidine has better biocompatibility, has a cell-penetrating function, a nuclear localization function, an endothelial cell targeting function and a certain pH buffer effect and can more easily escape from an endosome, so that the gene delivery effect is improved, the purposes of promoting cell migration and proliferation and promoting forming of blood vessels are achieved, and the current problems of non-virus vectors are solved.

Description

technical field [0001] The invention belongs to the technical field of gene carrier materials, and in particular relates to a histidine-based targeted penetrating peptide carrier. Background technique [0002] The promotion of rapid endothelialization on the surface of vascular materials by gene therapy is the process of delivering the target gene to endothelial cells through gene delivery vectors, and promoting the division and proliferation of endothelial cells through the effective expression of therapeutic genes in endothelial cells, thereby rapidly forming the endothelial layer. In the past few decades, non-viral gene vectors represented by cationic polymers, liposomes and dendrimers have been developed to some extent. People have prepared a variety of vectors with high gene transfer performance, but due to the common shortcomings of such vectors such as low biocompatibility and lack of biological activity, their application in gene therapy in vivo is also limited to a ...

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Problems solved by technology

However, its endosome escape ability is poor, which affects the effect of gene delivery

[0007] There are many gene carriers reported in the literature, but these carriers have ideal transfection effects on tumor cells and smooth muscle cells, but there is no effective gene carrier for vascular endothelial cells. It is urgent to develop a high-efficiency gene carrier for vascular endothelial cells. Developing ways to promote blood vessel formation

[0008] Currently, there are no reports of histidine-based targeted penetrating peptide carriers with low toxicity, targeting, nuclear localization, and endosomal escape functions.

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