Method for detecting CNV (copy number variation) marker of KLF8 gene of beef and application thereof

Abstract

The invention discloses a method for detecting a CNV (copy number variation) marker of a KLF8 gene of beef and application thereof. The method comprises the following steps of using DNA (deoxyribonucleic acid) of a blood whole genome of Jiaxian beef as a template; respectively amplifying the CNV area of the KLF8 gene and a reference gene BTF3 by a real-time fluorescent quantitative PCR (polymerasechain reaction) method; according to a formula of 2*log2 2<-delta delta Ct>, dividing results into an insertion type, a deletion type and a normal type, and identifying the CNV of the KLF8 gene. Displayed by the correlation analysis of production data of Jiaxian beef, the different copy numbers of the KLF8 gene have obvious influence to the growth and development of the Jiaxian beef, and the chest width of the deletion type individual is obviously higher than the chest widths of the insertion type and normal type individuals. The method has the advantages that the CNV closely related with production character of the beef is detected at the DNA level, and can be used as the important candidate molecular marker for assisted selection of the marker of growth character of beef, and the beef group with good genetic resources is quickly established.

Description

technical field [0001] The invention relates to the field of livestock molecular biology detection, in particular to a method for detecting the CNV marker of beef cattle KLF8 gene based on QPCR technology. Background technique [0002] With the rapid development of genomics and bioinformatics, animal breeding theory and technology are undergoing major changes, and the direction of beef cattle breeding is developing from conventional phenotypic breeding to molecular breeding. At present, the research on molecular breeding of cattle mainly focuses on marker-assisted selection based on molecular markers. Molecular breeding, that is, molecular marker-assisted selection (MAS), is a technology that uses DNA molecular markers to select genetic resources or breeding materials and improve the comprehensive traits of livestock and poultry. In livestock and poultry breeding, through the selection of DNA markers that are closely related to growth traits and quantitative traits, the pur...

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Problems solved by technology

[0007] At present, there is no literature report on the effect of KLF8 gene CNV on the growth traits of beef cattle breeds such as Jiaxian Red Cattle

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